铁蛋白

Results: There is a significant difference between the levels of Hb、SF、 FEP of model groups and normal group. The syndrome of every treatment groups is much better than physiological saline solution group, and recover gradually. The Hb、 SF、 FEP and IL-2、 IL-6 were recover gradually. But the improvement of AS two solstices pill add chalybeate group is comparatively obvious. Conclusions: AS two solstices pill add chalybeate has obvious therapeutic action on IDA model rats.

结果：造模后，各组大鼠Hb、血清铁蛋白、红细胞游离原卟啉与正常对照有明显差异，经治疗后症状表现上明显较模型NS对照组好，且逐渐恢复，Hb、血清铁蛋白、红细胞游离原卟啉、血清IL-2、IL-6水平逐渐恢复正常，其中以加味二至丸加铁剂组和当归补血汤加铁剂组最为明显。

Thus, it is important to enucleate the catalytic centre and mechanism of transferrins as a phosphatase, which may have a potential significant in the protein phosphorylation process.

据文献报导，传铁蛋白家族中的某些乳传铁蛋白和铁结合蛋白具有磷酸酶的功能，因此，研究传铁蛋白磷酸酶学功能位点和作用机制并进一步确定它们在磷酸化过程的作用具有重要意义。

The kinetic characteristics of the two eluates, DALF storing and binding organic dyes, and DALF binding haem were studied, which further demonstrated the proposition that it was the self-regulation of the protein shell not the complex surface of the core played a key role in iron release and storage.

通过释放铁的动力学研究，进一步证实了铁蛋白蛋白壳是非刚性结构，其柔性调节能力是铁储存和释放过程中的限制性因素，铁核表层的复杂结构与释放铁动力学过程呈复杂性无关。

The results showed that human transferrin could be expressed in these cell lines and mouse or goat mammary gland following transfection, and its expression level could be improved dramatically when it was driven by rabbit enhancer and promotor as compared with goat beta lactoglobulin promotor. Transgenic mice were generated by either microinjection or lentivirus infection.

结果显示所有表达载体均可指导人转铁蛋白在细胞以及小鼠和山羊乳腺中表达，兔转铁蛋白增强子与启动子组合可显著性增加人转铁蛋白的表达水平，且其指导表达的效率明显高于山羊β乳球蛋白启动子。

Two blood protein polymorphic loci were detected in transferring and posttremsferr from thirteen red steppes and eighteen hybrid of limousin and red steppe by polyacrylamide gel electrophoresis.

方法］采用聚丙烯酰胺凝胶电泳法检测了13头草原红牛和18头利×草杂交牛的转铁蛋白和后转铁蛋白2个血液蛋白多态性位点。

From the femur bone density analysis of protein expression, we can see that light chain lactoferrin, annexin decreased and the expression of enolase, ATP synthase, acetyl-coenzyme A reductase, the expression of troponin, may be related to ovariectomized femoral bone density in osteoporosis-related happened; strong bone of the femoral osteoporosis Po intervention effect may be related to reduced enolase, ATP synthase, troponin, CK-MB, acetyl phosphoglycerate enzyme, the expression of myosin, and the increase in lactoferrin light chain, pyruvate kinase isoenzyme, protein expression crown.

从股骨密质骨蛋白质组表达分析可知，乳铁蛋白轻链、膜联蛋白A3的表达下降及烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白的表达增强，可能与去卵巢后股骨密质骨骨质疏松的发生相关；强骨宝对股骨骨质疏松的干预效应可能与下调烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白的表达，和上调乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白的表达有关。

The result of whole mount in situ hybridization showed that MmeFer was located at the position of shell initiation in trochophore stage, indicating MmeFer plays a role in shell initiation in M. meretrix. The full-length of M. meretrix cathepsin B cDNA was cloned with 3'and 5'RACE. The temporal and spatial expressions of MmeCB mRNA were examined from trochophore to post larva stages by whole mount in situ hybridization. From L2 to L4 stages, MmeCB mRNA was detected in the digestive tract, suggesting a possible role of MmeCB in digestion.

根据构建的文蛤幼虫cDNA文库中提供的序列信息，从文蛤中克隆了与铁离子代谢密切相关的铁蛋白的全长cDNA序列；通过Real time PCR发现，MmeFer mRNA的表达量在贝壳形成前后有明显改变；整体原位杂交结果显示MmeFer mRNA在L1期的表达部位刚好是贝壳生成的起始部位，推断文蛤铁蛋白与文蛤幼虫贝壳初始形成密切相关。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

最后确认：股骨与骨质疏松发病可能相关的蛋白6个，分别为：乳铁蛋白轻链、膜联蛋白A3、烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白；腰椎与骨质疏松发病可能相关的蛋白5个，分别为：肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白；股骨与强骨宝疗效可能相关的蛋白9个，分别为：烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白、乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白；腰椎与强骨宝疗效可能相关的蛋白8个，分别为：碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶。

Iron regulatory protein, transferrin receptor and ferritin are main proteins in cellular iron metabolism.

铁调节蛋白、转铁蛋白受体和铁蛋白是细胞铁代谢中的重要蛋白质。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。