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CDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1335bP的片断编码444个氨基酸残基。

Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1 335 bp的片断编码444个氨基酸残基。

It is widely accepted that AP depolarize the plasma membrane and open voltage-dependent calcium channels , the calcium influx through VDCCs trigger the fusion of vesicles with plasma membrane and subsequent transmitter release.

现在被普遍接受的观点是当可兴奋细胞受到动作电位的刺激后，细胞的电压依赖性钙离子通道被打开，在动作电位的下降相引起细胞外钙离子的内流从而升高细胞内的游离钙离子浓度，触发许多细胞内的过程，譬如说神经递质分泌、信号转导和基因表达等。

The results show that, after treated with microwave radiation, the surface free energy of the powder is enhanced from 39.6mJm^(-2) to 41.8mJm^(-2), This is mainly caused by the rearrangement of electric charges on the grain crystal surface, and this phenomenon is coincident with the theory of electron transition caused by energy band splitting of the grain crystal surface.

测定结果显示微波辐射后硫酸钙粉末的表面自由能得到提高，从39.6mJm^(-2)增加到41.8mJm^(-2)，其主要原因是使硫酸钙表面能的极性成分得到了较大的提高。这一结果归于微波幅射引起晶粒表面的电荷重排，与晶粒表面能带分裂引起的电子跃迁理论是相吻合的。

Results All the NQs can activate the calcium release channel to some entent and induce Ca2+ transportation.The modulations of 1,4 NQ,1,2 NQ,1,4 NQ2S and 1,2 N are biphasic and depend on their concentrations.The mechanism of gating modulation depends on oxidation and reduction of NQ.The by-products of NQ during oxidation and reduction do not interact with RyR.

结果 所有NQ都不同程度地激活钙通道引起钙流释放；1，4NQ、1，2NQ、1，2NQ、1，4NQ 2S和1，2NQ4S对RyR的调节表现出强烈的浓度依赖性的双相作用性；NQ通过氧化还原作用调控RyR的门控机制；NQ在氧化还原循环中产生的超氧等伴生物没有参与RyR的相互作用。

It is the key factor regulating programmed cell death in pathogen,evocator and hormone responses.H_2O_2 has also been proved to play the signal role in defensive response to environmental stresses.H_2O_2 is known of directly regulating the expressions of numerous genes and some of these genes relate to plant defense and hypersensitive response.H_2O_2 also interacts with other signal systems,especially hormone signal system,and forms upstream or downstream components of hormone-mediated signal-transmitting passageways;more importantly,H_2O_2 also work to exert influence on or modify other secondary signals such as calcium signal between which and H_2O_2 many interactions take place;and both of these two signals regulate interactive tolerances of plants.

已知H2O2直接调节无数基因的表达，其中有些基因与植物防御和超敏反应有关。H2O2还与其它信号系统特别是激素信号相互作用，是激素介导的信号传导通路上的上游或下游组分；更重要的是H2O2还影响和修饰其它第二信使如钙信号的作用，在H2O2信号和钙信号之间发生众多的交互作用且这两种信号分子都调节植物对多种胁迫的交互耐性。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

Through researching for CF producing characteristics of ten kinds of iron ore sintering by means of small sintering test method and microstructure analyzing method, main factors of influencing producing CF were found. Under the conditions of basic capacity being 2.0 and other conditions being identity, limonite and hematite with loose microstructure as well as high ...

为此 ，采用微型烧结试验法和矿物组成及显微结构分析法研究了 10种铁矿石烧结的铁酸钙生成特性，找出了影响铁酸钙生成的主要因素，即在碱度为 2 。0及其它条件相同的情况下，结构松散的褐铁矿、赤铁矿及较高含量的 Al2 O3和 Si O2 均有利于 SFCA的生成。

The thermodynamic calculations of the Gibbs free energies for typical multiple phase equilibria in silicate systems were performed,including (1)melting reactions of silicate reactant in processing of the glass-ceramics;(2)sintering reactions in utilizations of nepheline syenite and high- flyash;(3)crystallizing reactions of tobermorite and xonotlite .

对硅酸盐体系的典型多相平衡反应进行了热力学计算，包括：(1)微晶玻璃制备过程中的硅酸盐熔融反应；(2)霞石正长岩和高铝粉煤灰利用技术中的硅酸盐烧结反应；(3)S iO2-CaO-H2O体系和KA lS i3O8-CaO-H2O体系雪硅钙石、硬硅钙石的水热晶化反应；(4)高铝粉煤灰和霞石正长岩烧结产物的溶解反应；(5)Na[A l4]-A l3-H2O体系和Na2SO4-Ca2-H2O体系中α-A l3和CaSO4.2H2O的析晶反应。

From the femur bone density analysis of protein expression, we can see that light chain lactoferrin, annexin decreased and the expression of enolase, ATP synthase, acetyl-coenzyme A reductase, the expression of troponin, may be related to ovariectomized femoral bone density in osteoporosis-related happened; strong bone of the femoral osteoporosis Po intervention effect may be related to reduced enolase, ATP synthase, troponin, CK-MB, acetyl phosphoglycerate enzyme, the expression of myosin, and the increase in lactoferrin light chain, pyruvate kinase isoenzyme, protein expression crown.

从股骨密质骨蛋白质组表达分析可知，乳铁蛋白轻链、膜联蛋白A3的表达下降及烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白的表达增强，可能与去卵巢后股骨密质骨骨质疏松的发生相关；强骨宝对股骨骨质疏松的干预效应可能与下调烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白的表达，和上调乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白的表达有关。

The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因，如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务，你就做你的事好了。