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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因,并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列,通过对克隆的降钙素序列的比较研究,结果显示同一科的鱼降钙素序列保守性较高,同时,根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究,结果显示在分类学上,分类地位较远的鱼,其降钙素相似性较低,分类地位越接近的鱼,其降钙素相似性越高;我们利用谷胱甘肽S-转移酶(Glutathions S-transferase,GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究,应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索,并将纯化的融合蛋白作为抗原,获得了高效价的兔抗鲑鱼降钙素免疫血清;生物活性研究表明,大肠杆菌表达的融合蛋白具有显著的降血钙作用。

The results showed that compared with traditional fluorite and phosphorous slag, phosphorous slag composite mineralizer was effective on reducing amount of f-CaO in cement clinker and improving burnablity of raw meals, but the effect was still obvious when sintering temperature and sintering time were increasing. The analysis of XRD, SEM and petrographic structure showed that phosphorous slag composite mineralizer decreased viscosity of liquid, promotes the formation of Alite in clinker and improves crystal form and size of minerals.

结果表明,与传统的萤石和磷渣矿化剂相比,磷渣与萤石复合的矿化剂可有效降低水泥熟料中游离氧化钙的含量,改善生料的易烧性;在燃烧温度与时间增加的情况下,改善作用仍然明显;通过XRD、SEM与岩相结构分析表明,磷渣与萤石的复合矿化剂降低了液相的粘度,促进了A矿的生长,对矿物的结晶形态有改善作用,有利于晶体尺寸的生长发育。

For example, to CaSiO〓, wallastonite and cyclowallastonite could be found simultaneously in glazes between the temperature range of 1125℃±10℃, only wallastonite came out below the range, and only cyclowallastonite appeared above the range. Similarly, melilite structure and clinopyroxene structure connected with crystallization temperature. Usually, higher crystallization temperature contributes to forming melilite with simple structure and lower crystallization temperature contributes to clinopyroxene with complex structure.

钙的偏硅酸盐,硅灰石、环硅灰石的转变温度区域为1125℃±10℃,组分适当的特定釉熔体,高于此温度出现环硅灰石,低于此温度出现硅灰石晶体结构;同样的,黄长石、辉石的出现也与温度有密切的联系,通常,晶体生长的温度较高有利于晶体结构简单的黄长石出现,晶体生长时温度较低有利于稍复杂的辉石结构的出现。

This thesis has systematically studies the microstructure of the sinter bearing fluorine of different compositions and in different sintering condition.(1) The main units of the Low Fluorine Sinter are magnetite、hematite、 SFCA、larnite、glass and pore. The cuspidine doesn't form for the low content fluorine, and the fluorine is in glass; Sinter basicity dominates the forming of the larnite and SFCA, when basicity is between 1. 72 and 1. 96, there are more larnites in the binder phase, and when the basicity is biger than 1. 96, there are more SFCA in the binder phase; the hematite appears like magnetite and usually is around pore.

为此,本文对不同组成、不同烧结条件的烧结矿样品进行了系统的显微分析和研究,其主要内容包括:(1)构成低氟烧结矿的主要组元有磁铁矿、赤铁矿、铁酸钙、硅酸二钙、玻璃体和气孔,由于含氟量的降低,枪晶石等含氟矿物并未形成,氟分布在后期凝固的玻璃体中,其中硅酸二钙和铁酸钙受碱度影响较大,硅酸二钙在碱度为1.72~1.96范围内形成较多,铁酸钙在碱度大于1.96含量增多,赤铁矿呈磁铁矿假象,多分布在气孔周围,各组元的生成顺序为:磁铁矿—硅酸二钙—铁酸钙—玻璃体。

While cytoplastic Ca2+ concentration is usually kept below 0.1-0.2 M, it will rise to function in intracellular signaling after excitation, with Ca2+ passing through Ca2+-permeable ion channels in plasma membrane and Ca2+-release channels in endoplasmic reticulum/sarcoplasmic reticulum (ER/SR. In pancreatic ??

静息状态下胞浆中钙离子水平很低,而细胞受到某些刺激后,钙离子可通过胞膜上的钙通透性通道或胞内钙离子库的钙释放通道提高胞浆中钙离子浓度,进而引起各种生理活动。

At present, several common sources of calcium, the calcium carbonate to the highest calcium content of the elements as much as 40 percent, much higher than calcium citrate, calcium lactate, calcium gluconate, dicalcium phosphate, calcium hydride.

目前常见的几种钙源中,以碳酸钙的元素钙含量最高,高达40%,大大高于柠檬酸钙、乳酸钙、葡萄糖酸钙、磷酸氢钙、氢化钙等。

The origin of glauberite attributes to various processes, including(1) direct crysllization from sulphate solution;(2) substitution of glauberite for gypsum;(3) transformation from hydrous glauberite; and(4) ion exchange between sodium sulfate and calcium-bearing mud.

钙芒硝的成因可归纳为:(1)直接从硫酸盐溶液晶出;(2)交代石膏成因;(3)从卤水中先沉积水钙芒硝后脱水变为钙芒硝;(4)硫酸钠溶液与含钙淤泥之间发生离子交换而生成。与菱镁矿紧密共生产出于海相地层之钙芒硝很可能形成于潮坪盐湖环境。

Cell membrane nonpermeable inhibitor of calmodulin -W〓-agarose- and the anti-serum of calmodulin can decrease the cytosolic calcium level, the results show that the endogeneous extracellular calmodulin may play an important role in maintaining and accelerating the cytosolic calcium level in pollen grain cell.

不能透过细胞膜的钙调素抗血清和大分子钙调素拮抗剂W〓-agarose处理后细胞内钙离子水平降低,免疫前血清对花粉细胞内钙离子浓度没有明显影响;进一步支持了上述结果,并且表明内源细胞外钙调素在维持和提高细胞内钙离子浓度方面发挥作用。

The effect of hydration of C 4A 3 in the presence of CaSO 4 and CaO as well as some admixtures was researched.

由于不同的掺和剂对硫铝酸钙的水化所起的作用不同,我们可以通过调节掺和剂的种类和加入量的多少来满足实际施工的需要。

A number of lines evidence indicate that calcium treatments maintain the structure and function of the cell walls, increase the fruit firmness, decrease the activity of the cell wall degration enzymes and ethylene production, maintain the integrity and stability of the membrane, activate some oxidases, induce phytolexin and regulate cell function as a signal mobecular.

钙的防腐机制在于对果蔬采后腐烂的控制,保持细胞壁的结构与功能,增强果实硬度,降低细胞壁降解酶的活性,抑制呼吸,降低乙烯生成量,保持细胞膜的完整性,激活体内的某些氧化酶,诱导合成植保素,作为信号分子,调节细胞功能。

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