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钙固定

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The ulnas of fetal mice were cultured for 48 hours in BGJb medium treated respectively with estradiol and purearin. After fixing long bones and decalcification, paraffin sections were made, and then performed immunohistochemical staining for determining the level of typeⅠcollagen protein in growth plates. Positive cell locations in the resting zone, proliferative zone and hypertrophic zone of epiphyseal plate were observed under light microscope.

剥离出胎鼠尺骨,置于BGJb培养基中,葛根素和雌二醇作用48 h后,将长骨固定,脱钙后作石蜡切片,进行免疫组织化学染色,光镜下观察骺板静止区、增殖区和肥大区阳性细胞定位,图像分析系统下测定免疫反应阳性细胞面积。

To add a little calcium alginate into boric acid cross-linkedpolyving akoholand permeate active sludge culture into the mix,and at last apply the mix on carrier to make film.

在加入少量藻酸钙盐的条件下,用硼酸交联聚乙烯醇,活性污泥培养物渗于其内,将其涂于载体上被固定成膜。

Under the field condition, effect of six amendments, including silican fertilizer, calcium magnesium phosphate fertilizer, lime and bone char and so forth, on in-situ chemo-immobilization of Cd in contaminated soils was investigated.

开展了硅肥、钙镁磷肥、石灰和骨炭粉等对镉污染土壤的固定修复研究。

The traditional approach to supplementing P for plants is to apply P fertilizer to the soil, but most of it may be readily immobilized or fixed by calcium ions in the lime soil or by iron and aluminum hydroxide in the acid soil, which results in a low use efficiency of P applied.

改善植物磷营养的传统方法是对土壤增施磷肥,但大部分磷肥被石灰性土壤中的钙和酸性土壤中的铁、铝氢氧化物固定成为无效态在土壤中积累起来。

The expression of VEGF, bFGF and BMP-2 were detected in femoral head through making use of immunohistochemistry and in-situ hybridization technique.Results The organization structure of experimental group was disorganized cracked and there were a great number of empty lacunas in bone trabecula.

所有标本取材后经固定、脱钙后,制成切片,光镜及电镜观察其病理变化及骨修复情况,并以免疫组化和原位杂交技术对其VEGF蛋白、bFGF与BMP-2mRNA的表达情况进行检测。

Methods:Mice of 6 and 8 postnatal days were sacrificed.The mandibles including the first molar were dissected and fixed.

出生后第6天和第8天的小鼠,取含有下颌第一磨牙的下颌骨,进行固定和脱钙,制备5μm的石蜡切片,原位杂交方法检测Msx2在小鼠牙根发育早期的表达。

Objective In order to understand the mechanism of stimulative effects of compression plate on bone healing in the treatment of mandibular fracture.

目的 通过观察狗下颌骨骨折不同内固定后不同时期钙元素及骨愈合强度的变化,对加压促进骨愈合的机制进行初步探讨。

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