钓

Drop ANCHOR,drop the CURTAIN

放下钓线，垂钓

Its speed in the water and chameleon-like color make it the ultimate challenge to catch, and because of its elusiveness, much of the joy of bonefishing is in searching for the fish.

它的速度和水中变色龙般色彩使它极限挑战钓、正因其捉，大部分的喜悦bonefishing是寻找鱼。

METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA，将其克隆入pET32a原核表达载体中，并在大肠杆菌BL21(DE3)中进行表达，然后以镍亲和层析法对表达产物进行纯化。

Many times, the movement of a curly tail will cause the extra enticement you need to catch them.

多次移动卷曲的尾巴会使你需要额外的诱惑钓。

At Esca, celebrated chef David Pasternak serves fish from around the world, but also commits himself to sourcing locally and the catch of the day might have been reeled in by his own hands.

艾斯卡的名厨大卫的餐厅里有来自全球各地的鱼，但他也采用在地食材，当天的渔获可能就是他亲手钓上来的。

I'm the sort of fisherman who's lucky to catch a fish at a trout farm, and my mother isn't any better.

我是那种即使是在养鱼池里也要老天保佑才能钓到鱼的人，我妈妈比我好不到哪儿去。

Widely distributed in Central America of fresh water and salt waters, the United States for the main freshwater aquaculture species. 1999 U.S. freshwater aquaculture production accounted for about 81 percent of the large warm-water species of fish, fork spots to fish tail as the Ministry of Agriculture to promote the country's fresh water breeding improved varieties have a very obvious advantages: First of Table no scales no small thorn, processing convenience and the other is the quality and refine, nutrient-rich, easy to digest and absorb the third is of miscellaneous eating habits, fast-growing, high yield, strong adaptability and easy to keep the fourth character is tame, like cluster activities , High density of aquaculture, particularly the pond custody and cage culture Fifth Tansi, easy fishing, Youdiao suitable for the development of leisure industry.

广泛分布于美洲中部的淡水和咸水水域，为美国的主要淡水养殖的品种。1999年约占美国淡水养殖总产量的81%，该鱼种是大型温水性淡水鱼类，斑点叉尾回鱼作为农业部向全国推广的淡水养殖的优良品种具有很明显的优点：一是体表无鳞没有小刺，加工方便；二是内质细嫩，营养丰富，容易消化吸收；三是食性杂，生长快，产量高，适应性强，易于饲养；四是性格温顺，喜好集群活动，可以高密度的养殖，特别是池塘主养和网箱养殖；五是贪食，易垂钓，适合发展休闲游钓业。

There he was, sitting with a fish pole in his hand.

他坐在那儿，手里拿着一根钓鱼杆。

Although five lines were in the water, five of the six fish were caught on one pole, the fish preferring its purple jig.

虽然我们放了五条饵，有一钓竿钓了五条，鱼似乎比较喜欢紫色的饵。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了