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(1) cerebral ischemical reperfusion injury rats'limbs motor function is variable. Acupuncture could promote lims'functional recovery.(2) PCNA masc cells is visible in cerebral ischemical semidarkness region. There is cell regeneration phenomenon. Acupuncture could strengthen injury region's PCNA expression, could profit injury recovery and functional reconstruction.(3) In ischemia semidarkness region for the model group and acupuncture group, PCNA masc cells percentage of 14days group is lower than 7days group. Along with the recovery of injury, cell multiplication is weaken.(4) In cerebral ischemia semidarkness region, there is VEGF masc cells and regeneration phenomenon. Acupuncture could strengthen injury region's VEGF expression, could profit protection after injury and blood vessel regenerate.(5) In ischemia semidarkness region for the model group and acupuncture group, VEGF masc cells percentage of 14days group is lower than 7days group. Along with the recovery of semidarkness region, ischemia and anoxemia state is getting improved, and VEGF is reduce.(6) As there are PCNA and VEGF masc cells in brain injured region, we could conclude that, after brain ischemical reperfusion injury, there are blood vessel regeneration phenomenon. Acupuncture could promote blood vessel regeneration, recovery blood supply sufficiently and quickly, and promote the recovery of brain injury region.(7)The VEGF masc cells percentage of inhibitor group is lower than acupuncture group. It state that the effect of acupuncture promote VEGF is partly depend on the existing of eNOS.

实验结论:(1)脑缺血再灌注损伤后大鼠的肢体运动功能发生改变,针刺可以促进肢体功能恢复;(2)脑缺损伤区可见PCNA阳性细胞,存在细胞再生现象,针刺可以增强损伤区PCNA的表达,有利于损伤的修复和功能重建;(3)针刺组和模型组14d时缺血损伤区PCNA阳性细胞百分比低于7d组,随着损伤逐渐得到修复,细胞增殖现象减弱;(4)脑缺血损伤区可见VEGF阳性细胞,存在内皮型细胞再生现象,针刺可以增强损伤区VEGF的表达,有利于脑损伤后保护和缺血区血管再生;(5)针刺组和模型组14d时缺血损伤区VEGF阳性细胞百分比低于7d组,随着缺血损伤的修复,缺血缺氧状态得到改善,产生的VEGF减少;(6)由于脑损伤区同时出现PCNA阳性细胞和VEGF阳性细胞,前者是增殖细胞的标志,后者是促进血管再生的重要因子,可以推断,脑缺血再灌注损伤后脑内存在血管再生现象,针刺可以促进损伤区的血管再生,更迅速而充分的恢复损伤区的血供,促进脑损伤区的修复;(7)抑制剂针刺组脑损伤区VEGF阳性细胞百分比与针刺组相比有不同程度的降低,说明针刺促进缺血损伤区VEGF表达部分依赖eNOS的存在。

The plates are obtained by two steps: compound felt and hot pressing shaping. The opening, mixture, carding, net-paving and needling process that affect shaping process of the blending fiber needle felt are investigated. Then it is concluded that the fiber nets from the BC-272B carding machines are uniform when the rolling speeds of licker-in, cylinder, doffer roller of the roller carding machine are 900r/min, 850r/min and 15.6r/min separately. The fiber needle felt is in a good condition when the paving nets have 8 layers, the type of the felting needle is 30, the density of needling is 1800end/m2, and the depth of needling is 3mm.

纤维复合板材分混合制毡和热压成型两步完成,分别探讨了开松、混合、梳理、铺网、针刺各工序对纤维针刺毡成型的影响,得出BC-272B罗拉梳理机的刺辊转速900r/min、锡林转速850r/min、道夫转速15.6r/min时,从梳理机下来的纤网较为均匀;铺网层数为8层、刺针型号为30、植针密度1800枚/m~2、针刺深度为3mm时,纤维针刺毡的效果较好,纤维复合针刺毡单位面积重量为200g/m~2。

Li Zhongren,Shen Meihong,Mu Yanyun,et al.Nanjing University of TCM,Jiangsu210029. Objective To explore into the probable mechanism of Alzheimer's diseaseby the acupuncture treatment combined with DGSYS of TCM.Methods Three kinds of model rats with dysmnesia which were made by injected into drug of scopolamine or NaNo 2 in the abdomen separately and injured at the hippocampal CA 1 in brian by drug of quinolinic acid treated by single acupuncture or acupuncture combined with DGSYS of TCM or DGSYS of TCM were observed separately,to compare different influence on dysfunctions of acuquisition and consoli-dation of memory induced by drugs and injure with nimodipineor the normal rats.

目的 探索临床针刺加当归芍药散治疗阿尔采默氏症的可能机理方法通过腹腔注射药物东莨菪碱或亚硝酸钠,或者用奎宁酸损毁脑海马CA 1 区诱发大鼠发生记忆力障碍,造成不同类型的大鼠&痴呆&模型,对照性观察针刺、针刺加DGSYS、DGSYS及NM对记忆障碍功能的影响和与记忆相关实验指标参的变化。

That to explore the general biologic mechanism of acupuncture through observation of ERK1/2 and P38 is a new try of studying the mechanism of acupuncture in the fascial point of view.

综上所述,本研究分别采用了HE染色、免疫组化、免疫印迹等技术手段,从针刺作用于机体的始动环节——针体对浅筋膜的牵拉和对肌肉的刺激着手,区别于以往的&穴位刺激——靶器官反应&的对于针灸研究的传统模式,且从观察ERK1/2和P38两种信号蛋白变化的角度,来探讨针刺作用于机体在生物学层面的普遍的机制,这是从筋膜角度研究针刺机理的一个新的尝试。

We didn\'t find activated points in cerebellum and deeper brain.3.Acupuncture of sham point can significantly activated BA2,6,8,13,21,37,40,44,45,47,putamen and other areas.Both the left and right side of the brain have activated points,but points on the right side are more than those on the left.Both sides of middle temporal gyms,parietal lobule,supramarginal gyms and the lentiform nucleus have obvious activated points.The activated points mainly concentrated in the cerebral cortex,the deep-activated are mainly in the putamen.4. Sham needling in sham point can significantly activate BA6, 8,9,10,18,21,37,40,43,44.The activated points are mainly distributed in the right side of the brain.The left side also has some activated points;5.In the comparasion of Acupunture and sham-needling in S J5,we find that BA8 and cerebellum have distinct regional activated points;6.In the comparasion of acupuncture in SJ5 and sham point,we find BA2 and left cerebellar regions have activated points;7.Sham needling in S J5 compared with sham point,we find BA7,8,9,18 and other areas have activated points,the main activated points are at the left brain.It is not difficult to find that the distribution of activated points are mainly in the middle brain,no obvious activated points at the temporal lobe.

结果:通过对数据的处理和分析,我们初步发现:1、外关穴真针刺能显著激活Brodmann area45、37、44、40、22、13、37、47区、海马、杏仁核、黑质等区域,小脑左侧更明显,左侧颞叶皮层激活点多于右侧,且脑部左侧深层激活点多于右侧;2、外关穴假针刺能显著激活BA46、44、41、13、40、37、19区等区域,激活点主要集中表现在大脑皮层,以颞叶为主,小脑及深部未发现明显激活点;3、非穴真针刺组结果分析初步表明,BA2、6、8、13、21、37、40、44、45、47区以及壳核等区域有激活点,大脑左、右侧均有激活点,但右侧更多,双侧颞中回、顶叶下小叶、缘上回及豆状核有明显激活点,激活点主要集中在大脑皮层,深部激活点主要在壳核;4、非穴假针刺能显著激活BA6、8、9、10、18、21、37、40、43、44区等区域有激活点,主要分布在大脑的右侧,左侧也有不少激活点,就其具体分布主要在颞叶和额叶,少部分分布在顶叶和枕叶;5、外关穴真针刺与假针刺对比发现,BA8区和小脑等区域有明显的激活点;6、外关穴真针刺与非穴真针刺对比发现BA2区、左侧小脑等区域有激活点;7、外关穴假针刺与非穴假针刺对比发现,BA7、8、9、18等区有激活点,主要反应点在左侧大脑的枕叶中回、楔叶,顶叶楔前叶及额上回、中回,就其分布不难看出主要在大脑中部,颞叶未见明显激活点。

A prominent visible blood vessel in the pontocerebellar angle was noted in the affective side, but no similar finding was noted in the non-affective side. In conclusion, acupuncture may improve facial spasm, this effect of acupuncture probable mediates via a antidromic impulse of facial nerve to decrease intensity of impulse that is orthodromic transmission from facial motor nucleus or from focal demyelization of neurovascular compression; acupuncture evoked cerebral cortex to produce a descending inhibiting impulse to inhibit the hyperexcitablity of facial motor nucleus to improve facial spasm.

结论是针刺可以改善颜面肌痉挛,针刺可能经由诱发颜面神经逆行的神经冲动减少颜面运动核或神经血管压迫之focal demyelination处往下而来神经冲动的强度;或者针刺诱发大脑皮质产生抑制性下行神经冲动抑制颜面运动核的过度兴奋来改善颜面肌痉挛。

By push-pull perfusion technique and radioenzymatic assay, it was found that after 10 min of acupuncture, pain threshold increased significantly, while the content of NA in the perfusate from the preoptic area was markedly decreased.

b。用推挽灌流技术结合放射酶学法,观察针刺镇痛时视前区NA释放的变化,从递质释放的角度,探讨视前区的NA在针刺镇痛中的作用。结果观察到针刺10min后,动物痛阈明显升高,此时视前区灌流液中的NA含量显著减少。

Clonidine (20ug/bilateral), an alpha agonist, produced similar effect as NA; while phentolamine (10ug/unilateral), an alpha antagonist, brought about an enhancement of AA, This phenomenon was observed in the acupuncture effective group, but not in the acupuncture uneffective one. However, propranolol (10ug/unilateral), a beta antagonist, had no effect on AA in these two groups.

结果表明,微量注射NA(0.5μg/单侧)能部分翻转针刺镇痛作用;α受体激动剂可乐定(20μg/双侧)也有同样作用;而α受体拮抗剂酚妥拉明(10μg/单侧)可以显著加强针刺有效组家兔的针刺镇痛作用,但对针刺无效组的痛阈无明显影响;β受体拮抗剂心得安(10μg/单侧)则对针刺有效或无效组的痛阈均无显著作用。

The age was around 25 to 45 years. Jianshi on Pericardium Meridian of Hand Jueyin and Weizhong on Bladder Meridian of Foot Taiyang was acupunctured."Active"/"silent" time of points were projected with Ziwuliuzhu Najia method. Acupuncture was done at the points "active"/"silent" respectively. The changes of NO content at the body points were detected. The difference of acupuncture in different time at the body points was compared.

年龄25~45周岁。分别选取手厥阴心包经的间使穴和足太阳膀胱经的委中穴作为针刺穴位,采用子午流注纳甲法推算穴位&开&、&合&时间,并分别在穴位&开&、&合&时进行针刺,同时在同侧经脉的郄门穴和承筋穴收集并检测NO含量的变化,比较不同时间针刺效应的差异。

In this paper, according to the basic idea of digital human body, acupuncture is seen as a kind of information input, and the change of brain activities after acupuncturing is taken as a response, to study the relationships between the input variables and output variables, and characteristics of brain activities.

本论文根据数字人体的基本思想,把针刺看作为是一种信息输入,把脑部在针刺后的活动变化看作为针刺输入响应,研究人脑状态变量与输入变量、时间的关系和人脑的时间行为。

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