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鉴定试验

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First, in the composite hypothesis testing, circular dispersion error which is more practicable than horizontal and vertical error is used to discuss some problems of the dispersion of impact points; and the critical area of decision in the dispersion problem is analyzed; the specific expression of Bayesian risk is worked out, which ameliorates the method that only can get the approximate solution by calculating numerical integration.

由此,本文主要应用Bayes方法对以下几方面进行了探讨:首先,在复杂假设下,本文利用圆散布误差这一精度指标对落点散布鉴定问题进行研究,它比以往所用的纵、横向偏差精度指标更具现实意义;分析了此鉴定问题的决策临界区域;给出其Bayes风险的具体表达式,对用数值积分进行近似求解的方法进行了改进;在此基础上,通过极小化试验的风险确定最优试验数n,并讨论了决策中犯两类错误的概率,给出了验前信息的表示。

MethodTo authenticate carefully the patients who have caught the nosocomial infection causing by the same bacteria within three weeks with the micro scan appraise systems of the France Bio Merieux API 20E and the USA Dade Behring. And test them by the K-B Medicine Susceptibility.

方法对集中在三周内发生同一种细菌引起的医院感染的病人采用法国梅里埃API 20E和美国德灵Micro Scan鉴定系统及K-B法药敏试验进行细菌的鉴定与药敏试验。

Methodto authenticate carefully the patients who have caught the nosocomial infection causing by the same bacteria within three weeks with the micro scan appraise systems of the france bio merieux api 20e and the usa dade behring.

方法对集中在三周内发生同一种细菌引起的医院感染的病人采用法国梅里埃api 20e和美国德灵micro scan鉴定系统及k-b法药敏试验进行细菌的鉴定与药敏试验。

Methods Germ tube test, chlamydospore test, CHROMagar Candida and API20 kit system were applied to separate non-Candida albicans strains from Candida albicans. Then PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 4 species of common Pathogenic non-Candida albicans (Candida glabrata, Candida parapsilosis, Candida krusei and Candida tropicalis), and the products were digested with the two restriction endonucleases (Msp Ⅰ and Hae Ⅲ) respectively. Results The four isolates consisted of 15 strains of Candida glabrata (7.50%), 7 strains of Candida parapsilosis (3.50%), 5 strains of Candida krusei (2.50%), 2 strains of Candida tropicalis (1.00%).

首先采用芽管试验、厚壁孢子试验、法国科玛嘉念珠菌显色培养基及API20CAUX酵母菌鉴定系统将分离自VVC患者阴道内的念珠菌菌株鉴定到种,然后采用真菌通用引物将4种常见非白念珠菌(包括光滑念珠菌、近平滑念珠菌、克柔念珠菌和热带念珠菌)进行PCR扩增,并选用MspⅠ和HaeⅢ两种内切酶对扩增产物进行酶切分析。

A new technology, Supercritical Fluid CO_2 Extraction, was also supplied to study the plant. The technological parameter was optimal. Total extract of SFE-CO_2 were 4.86 times of the essential oil extracted by SDE, main active extracted constituents were 1.36% high than SDE. The chemical constituents of SFE-CO_2 were also analyzed by GC-MS. 57 constituents were identified, 29 constituents of them were first discovered. 24 constituents were discovered in two extract. Fingerprint is a good technology to elevate the quality of herb medicine. The main active fraction can be extracted by SDE and SFE, Fingerprint was studied respective, chromatographic parameter was acquired. 22 peaks were assigned common peaks in the spectrum of essential oil, 17 peaks were assigned to common peaks in the spectrum of SFE extract.

采用现代共性关键技术超临界CO_2萃取技术,通过正交试验,优选了超临界CO_2提取牛至挥发油的工艺,并与传统的水蒸气蒸馏法进行了比较研究,结果显示:前者提取有效部位的收率为后者的4.87倍,主要有效成分的转移率是后者的1.36倍;采用GC-MS分析方法和NIST质谱库检索,对SFE和SDE所提化学成分进行比较,SFE分离鉴定了57种化合物,占提取物总含量的86.96%,其中29种化合物为首次从该植物中鉴定;后者分离鉴定了69个化合物,占提取物总量的98.28%,其中27种化合物为首次从该植物中鉴定;两种方法的提取物中共有成分25种,包括主要有效成分麝香草酚、香荆芥酚、对聚伞花素等。

Consider to make clear in recent years, second acid is calcic - Bowman does not move bacillus clinical bacterium of single born of the same parents of the detached rate that go up and verdigris holiday comparatives, have the tendency that year after year rises, the fundamental condition pathogenic bacteria that already became the infection inside the courtyard [1] , have to a variety of antibacterial content be able to bear or endure higher property of a medicine [1, 2] , for this, we are monitored and statistic analysed my courtyard to came 1998 2004 7 years a second acid is calcic - the clinical infection condition that Bowman does not change bacillus, to 14 kinds of antibacterial content be able to bear or endure property of a medicine and be able to bear or endure medical tendency, sum up a report to be as follows now. 1 material and method all data all come from 1.1 basic materials to came on January 1, 1998 my courtyard outpatient service was mixed on December 31, 2004 the specimen sending check of inpatient, via clinical microbiology the lab is fostered and classics appraisal is second acerbity calcium - Bowman does not move bacillus to affect case of illness, with clinical and commonly used antibacterial at the same time content makes the kill that medical quick detects. 1.2 bacteria appraisal is mixed medical quick experiment uses live thing VITEK32 of Mei Li dust is full automatic the bacterial appraisal of bacterial analysis system and its form a complete set gets stuck and medical quick card undertakes detecting, 1998~2000 year use GNI appraisal gets stuck, 2001~2004 uses GNI+ card.

近年探究表明,乙酸钙-鲍曼不动杆菌临床上的分离率和铜绿假单胞菌相当,并有逐年上升的趋向,已成为院内感染的重要条件致病菌[1],对多种抗菌药物有较高耐药性[1,2],为此,我们监测并统计分析了我院1998年至2004年七年间乙酸钙-鲍曼不动杆菌的临床感染情况,对14种抗菌药物的耐药性及耐药趋向,现总结报道如下。1材料和方法1.1基本资料所有资料均来自于1998年1月1日至2004年12月31日我院门诊和住院病人的送检标本,经临床微生物学实验室培养并经鉴定为乙酸钙-鲍曼不动杆菌感染病例,同时以临床常用抗菌药物作药敏检测的结果。1.2细菌鉴定和药敏试验采用生物梅里埃VITEK32全自动细菌分析系统及其配套的细菌鉴定卡和药敏卡进行检测,1998~2000年使用GNI鉴定卡,2001~2004使用GNI+卡。

All the isolates were first identified by different culture media including Niger seed agar. CACA agar, urease tests, ID32 identification kit, 37℃ growth test, and capability of forming capsule, and then the sequence of the LSUrDNA D1/D2 region were further analyzed.

分别以油菊籽、咖啡酸玉米琼脂、刀豆氨酸-甜菜碱-溴麝香草酚蓝培养基、尿素酶试验、37℃生长试验、ID32生化鉴定板和英膜形成试验等初步鉴定后,再经大亚基核糖体DNA的D1/D2区域序列分析进一步鉴定菌种。

The results Showed that twenty isolates have the following biological aspects:① Capability of forming capsule,② Growth at 37℃ within 3days,③ Forming brown colonies on Niger seed agar, CACA agar,④ Urea hydrolysis test positive within 96 hours.⑤ No blue colonies were found on CGB agar.⑥ The genus could be identified by ID32 identification kid, but the species could not.⑦ The sequence of the LSUrDNA D1/D2 region analyzed confirmed to be C. neoformans var neoformans.⑧ Serotype A and AD were found in these isolates, Serotype A was predominant(83.33%),⑨ Some biodiversities of isolates existed.

结果 20株菌皆具有以下特征:①形成英膜,②37℃3天内生长试验皆为阳性,③咖啡酸玉米琼脂和油菊籽培养基皆可以产生褐色菌落,④尿素酶试验96h皆呈阳性,⑤CGB培养基未见蓝色菌落生长,⑥ID32鉴定板可以鉴定到属,种的鉴定结果各不相同,⑦大亚基核糖体DNA的D1/D2区域序列分析证实皆为新生隐球菌的新生变种,⑧血清型仅见A和AD二型,以A型为多数(83.33%),⑨20株临床分离株随机扩增DNA多态性分析有一定的生态多样性。

In the present study, efficient identification methods for the main varieties of cowpea (Vigna unguiculata W.) and cauliflower (Brassica oleracea L.) in south of China, especially in zhejiang province, were investigated by using seven cowpea varieties (variety 1, Zhijiangtechang80;variety2, Zhijiangtechang90;variety3, Qiujiang512;variety4, Zhijiang228-2;variety5, Huajiang24;variety6, Yangjiang40;and variety7, Xingaochan8) and five cauliflower varieties (variety A, Longfenteda50tian;variety B, Yidaijinguang80tian;variety C, RuixuetedalOOtian;variety D, Qiuwang80tian;and variety E, Tezao50tian). The application of seed shape, seedling shape, rapid chemical identification and electrophoresis was performed, the main results were as follows.

为开展南方特别是浙江省主要蔬菜作物种子高效快速鉴定技术研究,本试验以七个豇豆品种品种1(之豇特长80,品种2(之豇特长90),品种3(秋豇512),品种4(之豇228—2),品种5(华豇24号),品种6(扬豇40号),品种7和五个花椰菜品种品种A(龙峰特大50天,品种B(一代金光80天),品种C(瑞雪特大100天),品种D(秋王80天),品种E(特早50天)为材料,从种子形态、幼苗形态、快速化学和电泳鉴定等方面对豇豆和花椰菜品种鉴定进行了研究,特别是对乳酸—聚丙烯酰胺凝胶电泳在这两种蔬菜品种鉴定上的可行性进行了研究。

The paper set a scientific indexs system that can be used in evaluating and analysing the drought resistance of dryland wheat.

通过18 a对旱地小麦品种筛选鉴定方法的研究与实践,参考国内外学者的经验,提出在区域化试验鉴定的基础上,采用田间直接鉴定与干旱棚鉴定、实验室鉴定相结合的抗旱性鉴定方法。

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