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Objective:To investigate the virulence,drug resistance and disinfectant resistance of Staphylococcus aureus isolated from surface of substance in medical stations.

目的:了解医疗站物表分离的金黄色葡萄球菌毒力、耐药性及消毒剂抗性,找出优势克隆株,为控制由金黄色葡萄球菌引起的交叉感染提供依据。

The culture of Micrococcus aureus in lag phase and decline phase are more sensitive to TFP than that of in logarithmic phase and stable phase.

抗菌蛋白对金黄色微球菌表现出杀菌作用,但无溶菌现象;在金黄色微球菌的四个生长阶段中,延滞期与衰亡期菌体对抗菌蛋白的敏感性强于对数期及稳定期。

Using micropipette aspiration technique and immunohistochemistry, the effects of staphylococcus. aureus and its metabolite on the rheological characteristics and cytoskeleton of PMN were studied.

利用微管吸吮技术和免疫组化染色,研究了在不同浓度金黄色葡萄球菌及其代谢液作用下多形核中性粒白细胞粘弹性的变化,以及在金黄色葡萄球菌作用下PMN细胞骨架的改变。

Resultsthe lowest spermicidal concentration of ethanol extract of rhynchosia volubilis lour roots on mouse and human sperm were 50mg/ml and 100mg/ml, respectively.the lowest inhibiting concentration of ethanol extract of rhynchosia volubilis lour roots on staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, and pneumobacillus were 1.0,1.0,4.0,4.0 mg/ml,respectively.conclusionthe ethanol extract of rhynchosia volubilis lour roots has spermicidal and antibacterial effect.

结果鹿藿醇提取物对小鼠和人精子最低杀精浓度分别为50和100 mg/ml;对标准菌金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌mic分别为1.0,1.0,4.0,4.0 mg/ml。对临床分离菌金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌mic分别为0.5,2.0,8.0 mg/ml。结论鹿藿醇提取物对小鼠和人精子有一定杀精作用,对临床常见致病菌有比较强的抗菌作用。

In particular, Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus has been implicated in some of these co-infections, and pneumococcus has been implicated in others.

在一些病例中发现合并感染了金黄色葡萄球菌(包括耐甲氧西林金黄色葡萄球菌,MRSA),在另一些病例中发现了肺炎球菌。

Oxford cup in vitro antibacterial medicine cup showed that it had vitro inhibitory action on beta streptococcus and colibacillus with crude drug content of 0.5g/ml; it had vitro inhibitory action on staphylococcus aureus,pneumococcus, beta streptococcus and colibacillus with crude drug content of 1.0g/ml,and decreased the death rate of mice caused by staphylococcus aureus.

牛津杯法体外抗菌试验表明0.5g生药量/ml时对乙型链球菌、大肠杆菌有体外抑制作用,1.0g生药量/ml时对金黄色葡萄球菌,肺炎球菌,乙型链球菌,大肠杆菌有体外抑制作用,降低金黄色葡萄球菌引起的小鼠病死率

The most common bacteria involved is Staphylococcus aureus, but other bacteria such as Staphylococcus epidermis and Streptococci are sometimes the cause.

最常见的细菌所涉及的是金黄色葡萄球菌,但其他细菌如金黄色葡萄球菌和链球菌表皮有时事业。

Results:The ultra structure of freeze-thaw Staphylococcus aureus was changed and the vacuole was formed in the cytoplasm;After recovery,the ultra structure of...

结果:冷冻解冻后金黄色葡萄球菌的超微结构遭到破坏,胞内有空泡形成;金黄色葡萄球菌修复后,其超微结构基本恢复正常。

The method of the soluble peptidoglycan isolation by short dose benzylpenicillin sodium and sephadex G-100 was founded; through the SLP reagent kit, the limulus test and the improved LOWRY reagent kit detecting, the prepared soluble peptidoglycan include shorter LPS and lower protein, its purity coefficient reach 93%.

建立并完善了青霉素诱导和Sephadex G-100凝胶柱法制备金黄色葡萄球菌可溶性肽聚糖的方法;经SLP试剂、鲎试验及改良的LOWRY试剂检测,所制备的金黄色葡萄球菌可溶性肽聚糖中LPS和蛋白的污染均较少,纯度达到93%以上; 3。

The antibacterial activities of most of fractions were similar to the pomegranate peel extract.(7) Respiratory inhibition experiment showed that the respiration of thallus was inhibited mainly through the hexose monophosphate pathway, which leaded to be short of NADPH, and induced metabolic function dysfunction. Growth curve assay showed that the pomegranate peel extract inhibited cytodieresis in logarithmic growth phase. After exposure to the pomegranate peel extract, bacterial protein was analyzed by SDS-PAGE. The results showed that protein synthesis was inhibited, especially for large molecular weight protein.

7呼吸抑制试验结果表明,石榴果皮提取物主要是通过抑制HMP途径,导致还原力NADPH缺乏,引起代谢功能障碍,达到抑菌的目的;加药组和正常组金黄色葡萄球菌生长曲线的对比结果表明,石榴果皮提取物能够抑制金黄色葡萄球菌对数生长期的菌体分裂;SDS-聚丙烯酰胺凝胶电泳分析表明,石榴果皮提取物能够抑制菌细胞蛋白合成,尤其对菌体内大分子量蛋白作用最明显。

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