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金黄色

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Results The high dose of QY(5.2g/kg)had obvious antifebrile effects as compared with the control group and the treated group had better effect of inhibiting bacteria than that of the control group.

结果 青英颗粒高剂量组(5.2g/kg)有明显解热作用;治疗组对金黄色葡萄球菌、金黄色葡萄球菌ATCC、甲型溶血性链球菌、丙型链球菌的抑制和杀灭作用优于对照组。

Results Ganmaofang had definite bacteriostatic action on standard strain of staphylococcus aureus and escherichia coli, and clinical dissociative strain of staphylococcus aureus, escherichia coli, klebsislla pneumoniae, Staphylococcus epidermidis, Staphylococcus intermedius, Staphylococcus simulans and beta streptococcus in vitro. Its MIC is between 0.075 g/mL and 0.2 g/mL. Ganmaofang could prolong the survival time of mouse infected by staphylococcus aureus and decrease the mortality of ill mouse, meanwhile it could enhance the survival rate of mouse infected by pneumoniae diplococcus.

结果感冒方体外对标准菌株金黄色葡萄球菌、大肠杆菌和临床分离菌株金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌、表皮葡萄球菌、中葡萄球菌、溶葡萄球菌、乙型链球菌具有一定的抑菌作用,MIC 0.075~0.2 g/mL,体内可延长金黄色葡萄球菌感染小鼠的存活时间和减少感染小鼠的病死率,对肺炎双球菌感染小鼠可提高感染小鼠的存活率。

Will come to rui Fang town water nan hole gold waterfall ,this seat at road side recite lean on have not ear teapot mountain cascade waterfall, because includes abundant pyrite with sulphur arsenic copper mine, lets earths surface submit golden, too is form yin and yang seas chief water source, ases gallop waterfall current let out but under shi jiu Like a piles up with gold mines waterfall, dazzling affecting, a screen limpid water curtain of straight rush gold yellow earths surface let people for a time rise play water idea, justs because heres water quality include huge amount of heavy metal material, for oneself safety, or prudent, gold waterfall locates in square self-improvement bridge side and long benevolence digging under this mountain six pithead, because in the early time at headstream picks up copper mine, mountain spring water with rainwater infiltration, slag sand deposits stream bed, and has fungus adhere multiply, hypsography differences compares big so form gold yellow waterfall spectacular sight, gold waterfall is gold melon stone local very characteristic a scenic spot, usually too very easy meets come this place screen nuptial dress new one.

来到瑞芳镇水湳洞的「黄金瀑布」,这座位在公路旁背倚无耳茶壶山的层叠瀑布,因含有丰富的黄铁矿与硫砷铜矿,让地表呈金黄色,也是形成阴阳海的主要水源,当奔腾的瀑水流泄而下时就像是一座堆满金矿的瀑布般,耀眼动人,一幕清澈的水帘直冲金黄色的地表让人一时兴起玩水念头,不过由於这里的水质含有大量重金属物质,为了自身安全,还是三思而后行,黄金瀑布位於本山六坑口下方的自强桥畔及长仁矿区,因为早期在上游采铜矿,山泉水与雨水渗入,矿砂沉积溪床,又有菌类附着滋生、地势落差较大所以形成金黄色的瀑布奇观,黄金瀑布是金瓜石当地很有特色的一个景点,平时也很容易遇到来此地拍摄婚纱的新人。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖;(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制:采用SLP试剂盒对提取物定性检测,应用动态浊度法鲎试验测定提取物中内毒素含量,采用改良LOWRY试剂检测提取物中蛋白的含量;(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件:青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

objective to analyze the drug resistance of staphylococcus aureusand provide suggestion for clinical rational use of antibiotics.methods secretion of those neonates suffered primary skin soft tissue infection and admitted during june,2005 to december,2007 was cultured and 113 strains of staphylococcus aureus were detected.we used k-b diffusion methods to determine their susceptibility to the common used antibiotics.results resistance rates of staphylococcus aureus to ampicillin/sulbactam,penicillin-g and cefazolin were 14.2%,40.7%and 6.2% respectively; there was no vancomycin-resistant strain.conclusion the resistance of staphylococcus aureus is not a serious problem in neonatal dermatic soft tissue infection.local treatment is the major approach in clinic,while general anti-infection consisting mainly of penicillin and first generation cephalosporin is chosen to avoid resistance mutation of staphylococcus aureus resulted from abuse of antibiotics.

多重聚合酶链反应快速鉴别葡萄球菌及。。。乳腺脓肿分离到葡萄球菌的鉴定和药敏。。。目的探讨金黄色葡萄球菌的耐药性,指导临床合理用药。方法对我院2005年6月至2007年12月新生儿原发性皮肤软组织感染患儿的分泌物进行培养,共检出金黄色葡萄球菌113株,并采用kb纸片扩散法对其进行常用抗生素的药敏试验。结果金黄色葡萄球菌对氨苄西林/舒巴坦、青霉素g及头孢唑啉的耐药率分别为14.2%、40.7%和6.2%,未发现耐万古霉素金黄色葡萄球菌。结论在新生儿皮肤软组织感染中金黄色葡萄球菌的耐药问题并不十分严重,临床处理应以局部为主,全身抗感染治疗宜以青霉素及i代头孢类抗生素为主,避免过度使用抗生素加速金葡菌的耐药突变。

We have investigated the conformational features of four short peptide fragments of Staphylococcal nuclease by homonuclear two-dimensional NMR and circular dichroism with a view to examining the early events in the folding process of SNase.

中文题名金黄色葡萄球菌核酸酶折叠起始位点:N-末端短肽段的NMR研究副题名外文题名 Folding initiation sites of staphylococcal nuclease:exploring the solution conformation of N-terminal fragments by nuclear magnetic resonance 论文作者戴继勋导师王金凤研究员学科专业分子生物学研究领域\研究方向学位级别博士学位授予单位中国科学院生物物理研究所学位授予日期2001 论文页码总数68页关键词金黄色葡萄球菌核酸酶蛋白质折叠核磁共振馆藏号BSLW /2001 /Q71 /89 本论文工作用四个金黄色葡萄球菌核酸酶N-末端短片段作为新生肽链折叠的模型,用同核二维核磁共振谱和远紫外圆二色谱研究了它们的溶液构象特征。

Some retrospective studies have proved that the clinical situations of Panton-Valentine leukocidin一producing Staphylococcus aureus infections are distinctive,especially necrotizing pneumonia caused by PVL-positive S aureus seems to be epidemic CA—MRSA stains causing skin and soft tissue infections,necrotizing pneumonia and lethal necrotizing fasciitis.

呼吸系统论文:近年来,国外对葡萄球菌杀白细胞素进行了大量的研究,多数研究显示产金黄色葡萄球菌感染有着特有的临床表现,特别是产PVL金黄色葡萄球菌相关性坏死性肺炎可能为一新现的临床疾病,由此PVL在金黄色葡萄球菌感染中的致病研究日益受到重视。

The minimal bactericidal concentration of PTKE on Escherichia coli, Staphylococcus aureus, Salmonella, Pasteurella multocida and Streptococcus agalactiae were 100, 25, 50, 25 and 50 mg/mL respectively. The isolation of EA, EB and EC had no antibacterial effect on Escherichia coli and Pasteurella multocida. MBC of EA on Staphylococcus aureus and Salmonella were 2.5 and 5 mg/mL, and MBC of EB on Staphylococcus aureus, Salmonella and Streptococcus agalactiae were 1.25, 5 and 2.5 mg/mL respectively, while MBC of EC on Staphylococcus aureus was 2.5 mg/mL. Polygonum taibaishanense Kung.

PTKE对大肠埃希菌、金黄色葡萄球菌、沙门氏菌、多杀性巴氏杆菌和无乳链球菌的最低杀菌浓度分别为100,25,50,25和50 mg/mL;而PTKE的分离物EA、EB和EC对大肠埃希菌和巴氏杆菌无抑菌作用,EA对金黄色葡萄球菌、沙门氏菌的MBC分别为2.5和5.0 mg/mL,EB对金黄色葡萄球菌、沙门氏菌和无乳链球菌的MBC分别为1.25,5.0和2.5 mg/mL,EC对金黄色葡萄球菌的MBC为2.5 mg/mL。

Specific IgY enhanced the phagocytosis of polymorphonuclear neutrophil leukocytes isolated from cow blood and milk with an optimal concentration of 1 mg/mL.The phagocytic percents of PMN in blood to S.aureus increased from 60.2%to 90.5%with IgY opsonizing,that of PMN in milk increased from 52.95%to 82.45%.The phagocytosis of PMN and macrophages in milk to S.aureus and E.coli were both enhanced by specific IgY.

该浓度IgY使外周血PMN吞噬金黄色葡萄球菌的吞噬率由60.2%升高到90.5%,乳中PMN吞噬金黄色葡萄球菌的吞噬率由52.95%升高到82.45%;乳中PMN和Mφ吞噬金黄色葡萄球菌的吞噬率分别提高29.5%和27.66%,吞噬大肠杆菌的吞噬率分别提高26.5%和26.36%,IgY对两种细胞的吞噬促进作用差异不明显(p>0.05)。

objective to study the effects of rabdosiae serrae compound preparation on staphylococcus aureus biofilm.methods minimal inhibitory coh centration were tested by doubling dilution,and simulate s.aureus biofilm in vitro be improved brown plate method.the effects of r.serrae compound preparation in different mic on s.aureus biofilm were determined and confirmed by scanning electronic microscope.single layer cells of human floss film were used to test the toxic effects of the compoud preparation.results the mic of s.serrae compound to s.aureus was 128?

目的 研究溪黄草复方对金黄色葡萄球菌生物膜的影响。方法采用试管二倍稀释法测定药物的最低抑菌浓度,用brown平板改良法体外复制金黄色葡萄球菌生物膜模型,并检测溪黄草复方的不同mic浓度对金黄色葡萄球菌生物膜的影响,扫描电镜确认。采用人绒毛膜单层细胞体外培养检测受试药物的毒性作用。结果溪黄草复方对金黄色葡萄球菌的mic值为128?

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