重组物

After 4 weeks age, BALB/c mice were vaccinated with recombinant germ orally,got the gastrointestinal contents and inoculated culture and counted number at 2,6,12,24,48,72h,meanwhile got gaster, jejunum、ileum and appendix to cryostat section,then cryostat section wasput under the fluorescence microscope to observe the distribution of the recombinant germ.

分别取胃肠内容物接种培养并计数，同时取胃、空肠、回肠和盲肠做冷冻切片，在荧光显微镜下观察重组菌的分布。

The recombinant hygrophilous aeromonad producing copolymer PHBHHx of HB and HHx contains beta-keto thiolase gene phhA and acetoacetyl-CoA reductase gene phhB.

本发明公开了一株产3-羟基丁酸和3-羟基己酸的共聚物PHBHHx的重组嗜水气单胞菌及其构建方法与应用。

Only Lamin A was observed to be able to be co-immunoprecipitated with 8B7 spectrin.Lamin A is a component in lamin and functions as a common anchoring site for many proteins.It plays important roles in maintaining normal morphology of nuclear envelope,presenting anchoring site for chromatin and reconstructing of nucleus.8B7 spectrin may function through interaction with Lamin A.

Lamin A是核纤层的组成成分，由LMNA基因编码，是核内许多蛋白分子的共同锚定物，在维持核膜的正常形态，提供染色质的锚定位点以及在核的重组过程中可能发挥重要作用。8B7血影蛋白可能经由Lamin A发挥功能。

Specific primers were designed according to gene NHX1 of Limonium gmelinii published on NCBI. Then, the gene was cloned by means of RT-PCR. Eukaryotic expression vector pCAM-BIA1301-NHX1 had been constructed, then transformed into tomato by Agrobacterium-mediated transformation method. Transgenic tomatos were obtained.

根据NCBI公布的大叶补血草NHX1基因序列设计特异性引物，经RT-PCR方法克隆得到大叶补血草液泡膜Na/H逆向转运蛋白基因NHX1，构建植物融合表达载体pCAMBIA1301-NHX1，重组质粒通过农杆菌介导转化番茄，经PCR和RT-PCR鉴定，获得转基因番茄植株。

AIM: To investigate the inhibitory effects of recombinant human Mullerian inhibiting substance on cell proliferation in human ovarian carcinoma cells (OVCAR8 and SKOV3 cell lines).

目的：探讨重组人苗勒抑制物对人卵巢癌细胞株OVCAR8及SKOV3细胞生长的影响。

Recombinant human Mullerian inhibiting substance suppresses the growth of MISIIR-positive ovarian cancer cells by inducing apoptosis and cell cycle arrest. We predict that rhMIS might be a new target to treat human ovarian malignancies.

重组人苗勒抑制物可以抑制MISIIR蛋白表达的卵巢癌细胞增殖、诱导其凋亡、阻滞细胞周期，这有望成为治疗卵巢癌的一个新靶点。

Though the molecular mass and the hypoglycemic activity of myristic acid modified HIDesB30 are both consistent with Detemir, the expression system, purification artwork and the methods of modification were different from Detemir.

本研究实现了含短C肽的人胰岛素原类似物HMPIDesB30在毕赤酵母中的高效分泌表达，表达量达1 g/L，分泌表达的重组蛋白HMPIDesB30未出现糖基化，未形成二聚体和多聚体，并具有生物学活性。

The somatic hybrids usually showed specific bands and combined all the bands of both parents. In combinations, some new bands were detected, and occasionally specific bands of parents were lost, which indicated that chromosome recombination and exchange happened after fusion. Additionally, in some combinations, specific bands from leaf parent were detected, which revealed the plants were of putative hybrid origin, because leaf protoplast cannot redifferentiate by both single culture and co-culture.

体细胞杂种一般表现为具有双亲的特征带，且综合双亲的所有带，但在部分杂种中检测到了双亲都没有的新带，也发现有丢失亲本的特征带或共有带的现象，说明融合后染色体发生了重组和交换；有的引物只检测到叶肉亲本的特征带，根据柑橘叶肉细胞无论单独培养还是共培养均不能再生的事实，可推测其为体细胞杂种或二倍体叶肉亲本型胞质杂种。

Methods:The conservative region of mTOR gene was inserted into pLXIN reversedly, then the vector was packaged in PT67 cells by transfection with lipofectamine and transfected to VSMC. The efficiency of antisense inhibition was verified,and the changes of p70S6k and 4E-BP1 were also determined at the same time. The proliferation activity was determined by flowcytometry and MTT.

采用脂质体介导转染包装细胞后构建mTOR的反义重组逆转录病毒载体(pLXIN-AmTOR)感染VSMC，检测mTOR及其下游底物包括真核细胞启动子4E结合蛋白1(4E-BP1)和核糖体蛋白S6激酶(p70S6k)的表达变化，以及VSMC细胞增殖周期和增殖活性的改变。

A SnaBⅠand NotⅠrestriction sites were introduced into NGF generespectively by PCR .After being amplified by PCR,this NGF genecontained SnaB Ⅰ and Not Ⅰ restriction sites at its 5'and 3'endsrespectively.

设计合成一对特异引物，分别带有 SnaBⅠ和 NotⅠ的限制性内切酶的识别位点，以 pPIC9K-NGF重组质粒为模板，用 PCR 方法扩增 NGF cDNA 片段。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。