重组物

ThemostpotentofthesetoxinsThe coding sequence of abrin a A chain gene was obtained by RT PCR and cloned into the expression vector pET28b.

成熟的ABRaA在大肠杆菌中得到高效表达，可溶性重组蛋白质的获得率达 4mg/L培养物，而且具有较好的纯度。

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein， Cp)序列设计引物，通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段，将目的片断克隆到原核表达载体pEGX-6p-1中，构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp，重组载体化大肠杆菌BL21，经IPITG诱导后，融合蛋白GST-cp得到了特异表达。

A maximum xylanase activity of 3, 030 U/mL was obtained from cell extract against birchwood xylan. The recombinant XT6 was partly characterized and was similar with those of the native enzyme in G.

重组表达的木聚糖内切酶XT6特性和天然酶相似，以桦木木聚糖为底物测定细胞提取物中木聚糖酶活性，最大活性高达3030U/mL。

The substrate coupling reaction system catalyzed by recombinanted CAR wasconstructed. It can be used for asymmetric reduction of 2'-chloroacetophenone and coenzyme regeneration with 2-propanol or 2-butanol as the cosubstrate.

5应用重组CAR不对称催化还原2'-氯-苯乙酮，通过加入异丙醇或仲丁醇作为辅助底物，建立了底物耦联反应体系实现辅酶NADH原位再生的反应体系。

To study the molecular structure and function of the PyNPase, and to construct a system for preparation of the enzyme for practical synthesis of nucleoside analogs, molecular cloning of the encoding the PyNPase from E. aerogenes strain EAM-Z〓 have now been completed.

我们通过以该酶的N端氨基酸序列设计上游引物，以不同来源PyNPase的C末端同源区氨基酸序列设计下游引物，采用PCR技术，扩增出该酶基因的编码区（约1206bp），并完成了其重组质粒的构建和核苷酸序列的测定，目前正在进行PyNPase表达质粒的构建。

In searching for RAPD marker linked to the genes controlling brachytic stem, 260 RAPD primers were applied to screen four parents of three combinations and RIL. Polymorphic bands revealed by the primer S-506 exhibited the best repeatability among all primers.

利用4个亲本和1个重组自交家系筛选260个RAPD随机引物，其中有1个引物S-506扩增出的多态性条带有较好的重复性。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

On other hand, specific fragments containing thermostable DNA polymerase gene were amplified from the genomic DNA of some strains of thermophilic bacteria isolated from Yunnan hot springs, and recombinant plasmids were constructed.

同时，我们还利用特异性的热稳定DNA聚合酶基因的扩增引物，从生长在云南温泉的嗜热菌总DNA中筛选出了能被此类引物扩增的特异性片段，并构建了相应的重组质粒。

In this study,by PCR amplification with the genomic DNA of three mapping parents,940 SSRs primers were tested for analysis their versatility and polymorphism in melon,cucumber and watermelon.

利用940对SSR引物，对甜瓜、黄瓜和西瓜三套重组自交系群体的作图亲本DNA进行PCR扩增，分析SSR引物在不同瓜类作物间的通用性与多态性。

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我尽力帮你找人帮忙。

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起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。