重测法

Nine super sweet corn inbred lines with abroad germplasm T1, T2, T3, M1, M2, M3, R1, R2, R3 were selected as tested lines, and original inbred lines B, C, D, 6, D2 as testing ones in this examination. 45 crosses were made by means of incomplete diallel method, and the genetic parameters of the groups and the combing ability of 10 traits, growth period, plant height, ear height, ear length, ear diameter, core diameter, baldhead length, rows per ear, kernels per row, single ear weight were tested and analyzed by means of NCⅡ design.

选用具有国外血缘的T1、T2、T3、M1、M2、M3、R1、R2和R3共9个超甜玉米自交系做被测系，以原有5个自交系B、C、D、6和D2做测验系，采用不完全双列杂交法组配出F1杂交组合45个，对生育期、株高、穗位高、穗长、穗粗、芯粗、秃尖长、穗行数、行粒数、单穗重10个性状按NCⅡ设计的原理和方法进行配合力测定与分析，并估算群体遗传参数。

Methods Bisulphate sequencing was applied to analyse the mcthylation of the CpG islands in the SNRPN gene locus.

方法应用重硫酸盐测序法对一例临床高度疑似PWS的患者及其父母的15q11-q13印迹中心SNRPN基因alpha外显子的10个CpG位点的甲基化情况进行分析。

Methods The rat models of diffuse brain injury were established by Marmarou's method. Real-time quantitative PCR, immunohistochemistry, dry-wet weight method, histological techniques and haematoxylin and eosin stain were used to detect expression of ICAM-1, the change of mRNA at different time phases, water containing in the brain tissue and the inflammatory infiltration respectively after diffuse brain injury.

采用Marmarou方法获得大鼠弥漫性脑损伤模型，实时定量RT-PCR、S-P免疫组化法分别测定ICAM-1蛋白和mRNA在外伤后不同时间点的表达变化，干湿重法测脑组织含水量，组织切片苏木精-伊红染色观测炎性细胞浸润情况。

Compared with gravitational method, the accuracy of TDR in de- termination of bulk moisture content on the Tibetan Plateau is:± 2.5% for silt and fine grained sand,±3.0% for clay and sandy clay and ±5% for gravel and pebble soils.

经与烘干称重法对比，TDR仪应用在青藏高原上不同融土类所测含水量值的误差范围：粉土和细砂为±2.5％，粘土和亚粘土为±3.0％，砂砾石土和碎石土为±5％。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。