重构的

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明： 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h，胞质突起率和去核率没有显著的差异，突起率可高达85.4%，去核率达到100%； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h，对去核效果没有显著影响； 3 对于体外成熟18~23 h的卵母细胞，随着成熟时间的延长，盲吸法的去核率降低，但没有影响秋水仙素诱导胞质突起的比率和去核率； 4 两种去核方法对重构胚的发育没有产生显著影响，但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

The last but not least, it is also owning to the rapid development in computer science and information technology. Such as, some computational task, which previously must be executed by using super-computer, now can be carried out by personal computer, and at the same time, the network speed and band width of data bus and input/output equipment have increased several times. In addition, the improvement of the high quality graphic displaying is available. All these provide strong support for cryoEM high-resolution reconstruction study.

目前，在低电子剂量的情况下，冷冻电镜照片可以记录到3-4 〓的信息，使用冷冻电子显微镜进行高分辨三维重构工作，已经获得6.5-8〓高分辨率水平的结果，可以分辨出蛋白质的α螺旋、识别出蛋白质的β折叠等蛋白质二级结构；其次是，三维重构算法效能的不断提高，使得对二十面体取向和中心参数计算的可靠性大大提高；再次是，由于计算机和信息技术迅猛发展，例如，以往需要使用大型计算机的计算任务已经可以被一般的个人电脑完成，网络速度、数据总线和硬盘等I／O设备的带宽成倍增长，以及图像显示能力的提高都为冷冻电子显微镜高分辨三维重构技术提供了强有力的支持。

Nowadays, in the low electron dose condition, the information about 3-4〓 can be recorded on the cryoEM micrograph, and the high-resolution reconstruction using the cryoEM has reached 6. 5-8〓 levels. The reconstruction can reveal the protein α helical and recognise β fold secondary structure. Secondly, it is due to the development in 3D reconstruction method, which can be more efficient to determine the orientation and center parameters. The last but not least, it is also owning to the rapid development in computer science and information technology. Such as, some computational task, which previously must be executed by using super-computer, now can be carried out by personal computer, and at the same time, the network speed and band width of data bus and input/output equipment have increased several times. In addition, the improvement of the high quality graphic displaying is available. All these provide strong support for cryoEM high-resolution reconstruction study.

目前，在低电子剂量的情况下，冷冻电镜照片可以记录到3-4 〓的信息，使用冷冻电子显微镜进行高分辨三维重构工作，已经获得6.5-8〓高分辨率水平的结果，可以分辨出蛋白质的α螺旋、识别出蛋白质的β折叠等蛋白质二级结构；其次是，三维重构算法效能的不断提高，使得对二十面体取向和中心参数计算的可靠性大大提高；再次是，由于计算机和信息技术迅猛发展，例如，以往需要使用大型计算机的计算任务已经可以被一般的个人电脑完成，网络速度、数据总线和硬盘等I／O设备的带宽成倍增长，以及图像显示能力的提高都为冷冻电子显微镜高分辨三维重构技术提供了强有力的支持。

PSNR.-images of two-dimensional discrete wavelet transform, transform Series is greater than or equal to 3, and reconstructi...

对图像进行二维离散小波变换，变换级数大于等于3级，并进行重构，最后计算重构图像的峰值信噪比

In the paper, an algebraic reconstruction algorithm based on magnetic resonance electrical impedance tomography was presented for 3-dimensional (3D) electrical impedance tomography, especially to image the 3D continuous impedance distribution of the head tissues.

文章给出了一种基于核磁共振技术的三维阻抗成像重构算法，并将该方法应用于人体头部组织电导率分布重构上。

The invention discloses a construction method of biorthogonal wavelet, which is based on Bernstein base and comprises the following steps that:(1) a lowpass filter at a discomposing and reconstructing end is expressed by Bernstein base;(2) numerical calculation of the lowpass filter is carried out;(3) the lowpass filter at the discomposing and reconstructing end is led to meet the conditions to reconstruct the lowpass filter entirely;(4) the parameter of obtained biorthogonal wavelet is calculated.

本发明公开了一种基于Bernstein基的双正交小波构造方法，包括如下步骤：(1)将分解与重构端的低通滤波器用Bernstein基来表示；(2)进行低通滤波器的数值计算；(3)使分解与重构端的滤波器满足完全重构滤波器的条件；(4)计算获得双正交小波的参数。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的，可在体外发育至孵化囊胚阶段，我们应用一种简便、快速的异种重构胚的鉴定方法，即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞（后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性）。

Under the domination of traditional consciousness such as experience, authority power and handworked skills, there exists the cognitive absence of technological rationality, therefore, the natural and social technologies used in reality exist the deficiencies such as sorts absencing, scattered utilization, content lag, etc., so it breaches the request of instrumental ralitonality to efficiency and value rationality to equity, and in a word, taxation need to be reconstructed on method systems according to the claim of technological rationality.

现代税收技术理性的重构首先应该起步于认识层面，必须坚持效率、公平和创新的原则；在实践层面上，应抓住当今先进的、核心的适用技术，分别以税收信息化建设为龙头实施自然技术理性的重构、以税收法制化建设为龙头实施社会技术理性的重构，以及按照系统论等原理实施自然技术与社会技术应用的有机结合，进而建立科学、合理、系统的现代税收技术应用体系。

Market of B2B of electronic business affairs basically was centered in natural resources of Alibaba, government and assemble 2006 market conditions, 2007 although market dimensions is very finite, but its affect trade the dimensions of value is close 2 trillion yuan, and 2008 this number can be achieved 2.5 trillion, the market is spent centrally also will drop stage by stage, forecast future 3 years B2B manufacturer of home appears on the market the company will achieve more than 10, catenary of zoology of B2B electron business affairs can undertake reframing, the segment that includes service of a lot of business can have such one reframes process, reframe in industrial catenary medium happening buy case will increase quickly.

电子商务B2B市场在2006年主要集中在阿里巴巴、治理资源和汇集商情，2007年市场规模尽管非常有限，但其影响的交易价值的规模近2万亿元，而在2008年这个数字会达到2.5万亿，市场集中度也就将逐步下降，猜测未来3年国内的B2B厂商上市公司将达到十余家，B2B电子商务生态链会进行重构，包括很多商业服务的环节都会有这样的一个重构过程，在产业链重构中发生的并购案将会快速增加。

Based on this, aim at the disadvantage on Wigner Distribution and Fractional Fourier Transform that can't restrain noise interference effectively when be used to detect Linear Frequency Modulated signals interfered by heavy noises, we proposed a improved WD. Simulation results show that this method can restrain noise interference effectively and improve the ability of LFM signals detection. On the other hand, in multi-component cases, WD suffers from the cross-terms, which may impair some of the auto-terms. In order to separate original multi-component signals into components and reconstruct them separately, we can use the method of optimal filtering in fractional Fourier domain. But this method also has a problem that if signals have aliasing in the fractional Fourier optimal domain as well, the reconstructed signals will be distorted. In this thesis, a novel method of quasi Fourier transform is proposed in order to solve the problem of multi-component signal reconstruction, and have good results.

在此基础上，针对Wigner分布和分数维傅里叶变换在处理高噪声背景下线性调频信号检测的问题时存在不能有效抑制噪声干扰的缺点，提出了改进Wigner分布的办法，该方法能够抑制噪声，提高信号的检测能力；针对Wigner分布在处理多分量非线性调频信号时存在干扰项的缺点，以及分数域最优滤波方法在处理多分量非线性调频信号分离重构问题时存在的局限性，该局限性表现为在对最优分数域上也有信号分量重叠的情况进行处理时重构信号发生畸变，提出了新颖的类傅里叶变换的方法，该方法对在最优分数域上也有信号分量重叠的情况也能处理，得到的重构信号失真很小。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！