重新利用

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

The following are included in the system: GIS client editing tools; use of the VBA editing environment in the ARCINFO platform; functions custom designed to meet customer needs; and use of the ARCSDE and ARCIMS tools in ARCGIS to manage data space in the E-map and provide to the internet. We have developed a module, using ichnography that is used in the system browser to manage microwave station information visually.

系统中与图形有关的设计和实现包括：GIS客户化编辑工具，我们主要采用了ARCINFO自带的VBA编辑环境，重新按照客户需求定制了自己的功能；使用ARCGIS中的ARCSDE和ARCIMS提供的工具对电子地图空间数据管理和互联网上发布；系统微波站图形方式浏览和管理，我们自己开发了一个模块，利用平面图作为页面的母层，平面图上需要表现的设备、维护人员等传输资源建立子层链接，通过链接可以浏览详细信息。

The theory of high-strain dynamic testing of pile bearing capacity. The basic calculation expression of pile high-strain dynamic testing is rederived in a simple way, especially the semi-empirical analytical expression of wave equation . Besides, theoretically it has been proved that it's not necessary to have identic section hypotaxis along pile, and the application condition and reasonable operation have been pointed out. the CASE comprehensive method is suggested to replace the traditional CASE method. author has made a lot of improvement for the mathematical model of the matching analysis measured curves in the business software CCWAPC and scientific research software JXWAPC of which the author is in charge.

关于高应变动力试桩法的理论研究以简明方式重新推导了高应变动力试桩法基本计算表达式，尤其波动方程半经验解析表达式，同时从理论上证明了CASE法并不需要桩身等截面假设，亦指出了该方法的适用条件及合理使用办法，并建议利用CASE综合分析法取代传统的CASE分析法；作者在其负责编制的商业软件CCWAPC及科研软件JXWAPC中，对波动方程拟合分析法数理模型进行了一系列重大改进，考虑了灌注桩桩周土体的变形特性和动荷截作用下的非线性效应、大直径桩的横向惯性效应。

After deep analysis of system structure and circuit design, we redesigned all of circuits of the thermal imager.

通过对Ⅰ类热像仪的系统结构及设计原理进行深入分析，将所有电路都重新进行了设计，利用FPGA的高速并行处理能力以及MCU的事务密集型的控制能力有效的改善了图象质量，消除了以往的图象缺陷，并且采取人机对话方式使调试、控制手段智能化，缩短了调试时间。

Two connecting methods,i.e. the method of code interface node and the method of calling dynamic link libraries, are introduced by analyzing the characteristics of Chinese data acquisition board and virtual instrument software Labview.A new method that can develop the driver of Chinese data acquisition board with Labview is presented.

所谓虚拟仪器实际上就是一种功能意义上的仪器，它充分利用计算机技术，在有限的基本硬件支持下，通过软件来实现各种仪器的功能，是具有仪器功能的软硬件组合，用户可以根据自己的需求更改或重新定义仪器的功能，其价格性能比也远远优于传统仪器。

Daulton topped Cliff Joyner a lengthy match last night, too, and will use his re-buy to advance to round nine.

道尔顿突破悬崖joyner冗长的比赛，昨晚太，并会利用他重新购买提前一轮9。

"Monkey Boys" remade the Monkey King Ming dynasty in Beijing 2006, they rebuild Monkey King which in subversion institutional feudalism dynasty into the background of globalization and electronic Monkey King post technology. No matter what games of their art is unthinkable, but the subject of Havocing in the welkin is also clear at a glance,"Monkey Boys" only increase a great many of entertainment and readability for new Monkey King that set up with the contemporary social background of consume, this generation can be seen as independent art students in a sign of China's rise, this generation of artists even see the network integration of this information, more use information itself, they let you feel the zooty and unclean art, also outpouring disdain with tradition art, or just make the tradition art become a back ground of art creative with themselves, only child artist is the first generation artist of the gibberish. This"control+n"group exhibition of artists forced to make fun of the lies about a monkey hero of the ancient Chinese legends and modern society link, trying to create a contemporary, and even is the pop cultural hero of network game.

公猿男孩'在2006年的北京重新制造了明版的孙悟空，他们将颠覆封建王朝制度的孙悟空改造成一个在全球化背景下的、在后科技背景下的电子版的孙悟空，不管他们的艺术游戏怎样的不可思议，但传统大闹天宫的主题还是一目了然，只不过'公猿男孩'他们增加了新孙悟空很多建立在当代社会消费背景下的娱乐性和可读性，这也可以被看作是独生一代艺术在中国崛起的一个标志，这一代艺术家更加看中网络上的这种信息整合，也更加利用资讯本身，他们就想让大家感觉到艺术的花里胡哨、艺术的模棱两可，甚至也流露出对传统艺术的不屑一顾，或者只是将传统艺术变为他们自己艺术创作的一个背景，独生一代艺术家首先是无厘头的一代艺术家，此次'control+n'群展的好玩之处还在于使艺术家强行的将一个中国古代的传说中的猴子英雄和现代社会链接，力图打造一个当代版的、甚至是网络游戏版的流行文化英雄。

The town's ailing infrastructure 4 was overhauled 5 in the 1870s by Alexander 'Boss' Shepherd, whose extravagant use of federal funds and penchant for steamrolling anything in his way led to a crackdown by Congress that robbed DC of self-government for another 100 years. For the citizenry, it was a high price to pay for a city beginning to look like it might fulfil L'Enfant's original vision of a world-class capital.

整个城市萎靡不振的基础建设在1870年代由 Alexander 'Boss' Shepherd 重新翻修，但由於他过分利用联邦基金，同时强烈主导整修一事，导致美国国会迫使 DC 在接下来的100年，成为一个自治的区域；对於当地人民来说，这算是付出相当高的代价，因为原本这个城市将是一个世界级首都，但此时却沦为如此的情况。

Lastly, we discuss the energy-band structure of ultracold atoms in optical lattice by means of Green function method and in addition, procure the superfluid-Mott phase transition condition in mean-field approximation which is in agreement with the result in the literature.

最后利用格林函数方法讨论了光格子中超冷原子的能带结构，根据Mott相存在能隙的判据我们在平均场近似下重新得到superfluid-Mott相变条件，该结论与相关文献一致。

This dissertation focuses on the spherical wavelet expansion of the earth gravity field and its application. Based on the concept of the integration approaches and radial basis functions the spherical wavelet is put forward, with that the earth gravity field is described in a new way, and some other researches are conducted, they are: spherical wavelet regularization, multiscale approximation of satellite gravity measurement, down continuation of airborne geavimetry data, the least squares properties of geopotential wavelet approximation, the spherical wavelet solution of two kinds of BVP and spherical selective multiscale reconstruction.

本文围绕地球重力场的球面小波展开及其应用进行研究，在积分逼近与径向基函数概念的基础上引入了球面小波，利用球面小波对重力场展开进行了重新表示，在此基础上进行了球面小波正则化、卫星重力多尺度逼近、航空重力数据向下延拓、重力场球面小波逼近的最小范数性质、两类边值问题的球面小波实用解、球面选择多尺度去噪方法等方面的研究。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！