重复的

The affinity among hybridizable species can also be speculated by the extent of homology of their repetitive DNA.

高等植物DNA的很大部分是由重复顺序所构成，本文讨论了检测亲本及子代基因组内重复顺序的异同程度作为远缘杂交的分子水平指标是简便可行的。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

Regarding to test method standard, the setting of Chinese woolen standard is not systemic. For instance, the amount of test method for all kinds of woolen fiber quantitative analysis is far more numerous in China and the principles of the standards are iterant.

国内标准中，各类纤维定性定量分析（尤其是各类毛纤维的定量分析）方法标准以及毛纤维的某些测试方法标准的数量多，内容却分散或重复；毛织物产品标准按生产工艺与织物成分划分，同类织物间考核要求重复，考核项目多，标准格式的兼容性差。

They play important roles in studying the liveness and fairness of Petri nets.

本文在对Petri网进行结构变化的基础上，研究了T-不变量、可重复向量、死锁间的关系，给出了一些新的算法去求解一个网的可重复向量与死锁。

This paper indicatesthat the Triticum-Agropyron octaploid species and the alien substitute line containAgropyron DNA sequence in different proportion. This result is consistent with thecytological and genetic analysis.

高等植物DNA 的很大部分是由重复顺序所构成，本文讨论了检测亲本及子代基因组内重复顺序的异同程度作为远缘杂交的分子水平指标是简便可行的。

The organization of genomes of A. Sinensis and A. Mississippiensis have been examined, Both reassociation curves are very similar. There are only small fractions of highly repetitive sequences in genomes.

通过DNA复性动力学的分析，测定了扬子鳄和密河鳄基因组的组织结构，两者的复性曲线非常想象，高度重复DNA含量很少，而中度重复DNA序列各占基因组的30％左右。

Two molecular forms of Xenopus hatching enzyme, 60kD and 40kD molecules, was obtained during preparation and purification. Both of them were verified as the hatching enzyme molecules, using anti GST UVS.2 antibody as the probe. 60kD molecule was digested or autodigested easily into 40kD molecule during purification. It was indicated that 40kD molecule was probably derived from 60kD molecule with its two CUB repeats lost, and the two CUB repeats may play an important role in recognizing and/or modifying of the molecular structure of vitelline envelope.

在分离纯化非洲爪蟾孵化酶时，得到了60kD和40kD两种分子，用孵化酶的特异性抗GST-UVS.2抗体进行Western杂交的结果证明二者均为孵化酶分子。60kD分子很不稳定，在纯化过程中极易降解，40kD分子可能是由60kD分子经过降解或自身降解丢失了两个CUB重复区而形成的，而CUB重复区很可能在对受精膜分子结构的修饰或改造中具有重要作用。

The author finds that there are many prolems information is obturated and cannot be shared, repeated design and consruction after the investigation on committee of planning and design and the managements.

作者对我国城市管网的规划设计部门，管网的运行维护管理部门等进行了系统的调研，发现各专业之间的信息闭塞、信息无法共享、重复设计、重复施工等问题普遍存在。

To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

为了简单高效检测HD基因开放阅读框5'端n三核苷酸重复序列，建立快速准确的亨廷顿病(Huntington disease， HD)基因诊断方法，应用TaKaRa LA Taq DNA聚合酶配合GC buffer扩增HD基因包含n重复序列的目的片段，非变性聚丙烯酰胺凝胶电泳检测后回收n拷贝数异常增多的目的片段，再次PCR扩增后将产物连接至T载体，进行DNA测序确定CAG的拷贝数。

More than 10 QTL were detected in heading date, plant height, tassel branch number, tassel length, ear height and stem diameter, respectively.

抽穗期、株高、雄穗一级分支数、雄穗长、穗位高和茎粗检测到的QTL达到了10个以上，且平均一半以上的QTL能够被重复检测到，被重复检测到的QTL的平均贡献率在10%以上。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！