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Under optimal reaction procedures and reaction system, genomic DNA of a series of 10 times reference culture diluents of salmonella enteritidis extracted through poaching cracking process is taken as a template to carry through fluorescence quantitative PCR , and then specification curve is drawn. Based on linear relation R and augmentation efficiency E, whether the repeated sample data are consistent and whether initial templates with different copy numbers have the same augmentation efficiency are judged.

在优化的反应程序和反应体系下,以水煮裂解法提取的肠炎沙门氏菌标准菌株10倍系列稀释液的基因组DNA为模板,进行荧光定量PCR,绘制标准曲线,根据线性关系R 2 和扩增效率E来衡量重复样品数据是否一致和不同拷贝数的初始模板是否具有相同的扩增效率。

Methods Spermatocytes, early stage spermatids and the later stage spermatids were separated from the adult rat testis by the velocity gravitative sedimentation technique. Using the whole mount electron microscopy, SDS-PAGE and Southwestern blot, the morphology, components of NM-L-IF and the relationship between an A-T repetitive DNA fragment and the proteins of NM-L-IF in those cells were analyzed.

利用整装电镜技术、SDS-PAGE和Southwestern blot等技术观察与分析经单位重力沉降法分离的大鼠精母细胞、早期精子细胞和晚期精子细胞的NM-L-IF的形态结构、蛋白组成及其与A-T重复序列的相互关系。

Three Cases with hydronephrosis were located in left kindey, and 3 cases in bilateral kidneys. Of 5 kidneys in 4 cases of ureteropelvic junction obstruction, Anderson-Heny's procedure was performed in 4 kidneys, and nephrectomy was performed in 1 hydronephrotic kidney because the renal function had lost. Cohen's procedure was performed in all 3 individual hydronephrotic kidneys in 2 cases.

病因及治疗:肾盂输尿管连接部梗阻4例5只肾,4只肾做了Anderson-Heny's手术,1只肾无功能行肾切除术;2例3只肾为巨输尿管症,均行Cohen's法输尿管膀胱移植术,其中1例合并膀胱重复畸形,手术中同时行共同壁切除;l例出生后肾积水渐减轻,未特殊处理,正在随访中。

He put forwards that the article 4 of crime of L/C fraud in Chinese Criminal Law isn"t in compliance with the principle of penalty according to criminal law, and it will destroy L/C system. The principles of fairness, honest and credibility and prohibition of civil right abuse in General Rules of Civil Law have the feature of indetermination. The litigation of civil right attached to criminal procedure and litigation property save in the Code of Criminal Procedure, in view of their imperfection, cause the repeating capital frozen by police bureau, procuratorate and court. In view of low requirement of civil litigation capital save in Civil Procedure law, the frequent capital frozen happens all the time. Summary of Panel Discussion Concerning Chinese Coastal Area"s Economic and Judicial Work, owing to its low status and evidence"s inexplicitness of issuing ban, results in judger"s unrestrained jurisdiction power. Therefore, this author concludes that the characteristics of fragmentsand imperfection of Chinese L/C laws are disadvantageous to the perfection of the L/C legislation system ,and can not instruct judges to try the cases of L/C fraud effectively.

由于《美国统一商法典》的第五章关于信用证欺诈的规定相对我国法律而言比较完善,笔者在本文对其也作了论述,以期对我国未来的立法有所启示;第三部分主要分析了我国目前的有关信用证欺诈的规定,并指出《刑法》的有关信用证诈骗罪的规定的第4款不符合罪刑法定的要求,它对信用证的运行机制有极强的破坏性;《民法通则》中与信用证欺诈有关的公平、诚实信用、禁止权利滥用原则有其适用时的不确定性;《刑事诉讼法》关于刑事附带民事及诉讼保全的规定,由于规定的不完善,导致公、检、法等各机关对信用证的重复冻结;由于《民事诉讼法》对财产保全的条件要求过低,导致实践中信用证冻结频繁发生;《会谈纪要》由于其效力低和对发布禁令要求的证据并不明确,导致实践中法官的自由裁量权无限扩大。

Objective To evaluate the accuracy of partial CO2 rebreathing technique, and the effect of intrapulmonary shunt.

目的 探讨部分CO2重复吸入法测定危重病患者心输出量的准确性及入肺内分流量对其影响。

Month-old Wulong geese were divided into four groups (8 for each) in a random-designed experiment to study the effect of Euchsaena mexican schrad diet on N equilibrium and digestibility of Ca and P of Wulong geese. Each group was fed with the isoenergetic and isonitrogenous diet of different CF levels (6.02%, 6.86%, 8.66% and 10.41% respectively).

将32只9月龄健康五龙公鹩完全随机分为4组,每组8个重复,各组分别给予不同纤维水平的同蛋白、同能量的墨西哥玉米干草粉结构日粮,采用全收粪法进行消化代谢试验,研究不同水平的墨西哥玉米干草粉纤维素源对五龙鹩的氮平衡及钙、磷消化率的影响。

MethodThe depths of plough layers, sunshine effects in seedling stage, resistances to Ernst damage, microclimatic effects such as soil moisture, rent system in filling stage, growth periods and grain weights of maize planted on ridge and in furrow were comparatively observed. The random arrangement was adopted in comparative observation with 4 replications.

方法]对种垄台与种垄沟玉米的耕作层温度、出苗期日照效应、抗御春秋霜低温冷害、土壤墒情等微气候效应以及玉米灌浆期根系生长状况、生育期及粒重进行对比观测,观测采用随机排列法,同时进行4次重复。

Objective:This paper proposes an iterative noncentral procedure for the sample size determination in log rank test and compares it with the LachinFoulkes procedure. Methods: Samplings are performed with a prescribed size.

本研究首先按临床数据实际分析过程说明设计参数赋值依据和生存研究所需样本量测定原则,然后提出迭代非中心法,即借助重复抽样估计其非中心参数,测定所需样本量,并同基于指数分布的方法进行比较。

At present, film photocatalytic is studied mostly for treating with water pollution. Because the film will not bring the secondary pollution, and can be used again. ZnO film and TiO_2 film is mostly used as photocatalyzer, but they have some disadvantage, as they can only absorb little of the sun-light energy, the photoluminescence exciton compounds mostly, and they can be eroded easily by light. Complex semiconductor film is a good method to ravel out these problems.

目前,在光催化治理水污染方面,膜的光催化是一个研究的热点,因为膜催化不存在二次污染且便于回收和重复利用,研究较多的是ZnO薄膜和TiO_2薄膜,但是单一组分的薄膜存在光能利用率低,光激子复合率高,易发生光腐蚀等缺点,复合膜是解决该问题的有效途径,我们提出了全新的复合薄膜的制备方法—混合溶胶法。

Under the improvement type of a thesis perform algorithms, it will not need to rescan the whole database each time when there are newly-increased materials. It usually needs to scan the newly-increased trade materials in order to improve the efficiency of prospecting.

因为在过去的资料探勘技术中,在建构决策树的过程中,若有新增或删除交易资料时,则必须重新计算一次,将造成前次探勘计算的重复及资源的浪费,所以本研究的改良式演算法当有新增资料时将不用每次都要重新扫描整个资料库,绝大部分只需要扫描新增的交易资料,以提升探勘效率。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力