重复法

The short tandem repeats is a kind of DNA sequence formed repeatedly and connectively subjected to a core unit of several base pairs.The non-interfered multi-PCR was applied with 3 loci in the same reaction system.By using the technique of denaturing polyacrylamide gel electrophoresis and silver stain,the allele frequencies distributions of 3loci:F13A01,FESFPS and vWA in 10 minority populations only resided in Yunnan Province:Bai,Dai,Achang,Jingpo,Deang,Lahu,Bulang,Hani,Pumi and Jino.A satisfactory result was obtained and some significant genetic differences were noticed in different populations.

短串联重复序列是由几个碱基对作为核心单位串联重复形成的-类DNA序列，应用3个位点在同一反应体系中进行互不干扰的多重PCR，采用高分辨力的聚丙烯酰胺凝胶电泳分离、银染法显影技术，对云南省特有的白族、傣族、阿昌族、景颇族、德昂族、拉族、布朗族、哈尼族、普米族和基诺族等10个少数民族的F13AO1、FESFPS和vWA3个位点等位基因的基因频率分布进行了调查，获得了满意的结果，在不同群体中发现一些有意义的遗传差异。

For a four PRFs' radar system, the resolved results calculated by the residues' difference look up table method from any two of the target distances detected are equal.

对于一个四重脉冲重复频率的雷达系统，利用真实目标在任何两个脉冲重复频率上检测得到的视在距离，经过余差查表法匹配解得的目标距离是相同的这一特性，可以对多目标进行解距离模糊。

To weaken the influences of the position-dependent periodic disturbances and parameter uncertainties, the robust repetitive controller was designed, which associated structure singular value theory with the discrete-time repetitive control theory. The problem of robust repetitive control was transformed into one of H∞ optimal control using linear fractional transformation. The robust repetitive controller was achieved using D-K iteration procedure and made the servo system have stability, robustness and tracking performance.

为了削弱周期性扰动及参数变化不确定性的影响，将重复控制和结构奇异值理论相结合，利用线性分式变换(linear fractional transformation，LFT)将鲁棒重复控制问题转换成H∞最优控制问题，采用D-K迭代法设计鲁棒重复控制器(robust repetitive controller，RRC)，实现系统的稳定性和鲁棒性，提高系统的跟踪特性。

Our aim was to compare the difference between body plethysmography and helium dilution method detecting the FRC repeatedly.

现比较体描法与密闭式氦稀释法的重复呼吸法测定功能残气量之间的差异。

The holistic design of the system and resolved the designs of software and hardware based on VXI bus are put forward according to the demand of test system. The hardware and software is designed, including the formation and design of hardware system, visual instrument software architecture, program design for instrument driver, high speed real-time data collection. In the dynamic test program, the idea of"software is instrument"was applied to meet the diverse test demands. Last, it is proposed that the VXI test system be developed to more totalization and networked. The basic theory of system identification was introduced briefly at first. The principle of spectrum analysis system identification based on Wiener-Hopf equation was expatiated. and the questions in applying Peusdo Random signal as spectrum analysis methods stimulate signal was studied, The generation principle of the peusdo random signal is researched, the characteristic of peusdo random are analyzed from time domain and frequency domain, the inverse repeat peusdo random signal is brought forward to make up the shortage of ordinary peusdo random signal. And a new chaos genetic algorithmis presented to solve the unprecise problem of the traditional method. In the servo valve dynamic characteristic test system, the spectrum analysis methods was applied successfully, the servo valve dynamic characteristic could be acquired rapidly and exactly, the test results were analyzed and the ameliorate methods were brought forward at last.

结合航天某集团液压测试中心测试系统的需求，提出了基于VXI总线的液压测试系统的整体设计方案，完成了测试系统的硬件设计与软件设计，包括硬件系统构成与设计、虚拟仪器软件结构、仪器驱动程序设计、高速同步实时数据采集等内容；以伺服阀动态特性测试为例设计了测试模块，该模块体现了VXI总线仪器软件开发所倡导的"虚拟仪器"的思想，以软件的灵活应用满足了不同的测试需求；结合自动测试系统的发展，研究了VXI测试系统与网络技术结合，实现各种软硬件资源共享，进一步提高设备及数据的利用率和系统的扩展性，使自动测试向综合化、网络化方向发展；研究了伪随机信号作为谱分析法辨识的激励信号时存在的问题，讨论了伪随机信号的产生机理，从频域和时域分析了伪随机信号的性质及其应用，提出了用逆重复伪随机信号弥补了普通伪随机信号的不足，并以混沌遗传算法对逆重复伪随机信号产生过程中的参数进行优化，可以更合理而不是凭经验产生需要的逆重复伪随机信号。

Methods Gene analysis by analysis susceptibility, ERIC-PCR techniquea and plasimid fingerprinting were put into practice for the detection of gene variation of 10 strains of Salmonella enteritidis. The similarities between different strains were obtained by software NTSYS-PS 2.0 for cluster analysis.

药敏试验采用K-B法，用碱裂解法提取细菌的质粒DNA，采用肠杆菌重复基因间隔重复共有序列技术对10株肠炎沙门菌进行基因分析，运用NTSYS-PC2.0软件进行同源性比较，聚类分析。

False positive were lowered to an acceptable rate by this protocol, therefore, a big amount of work on fingerprint to get rid of false positive clones can be avoided. When whole BAC clone containing one locus of a multi-loci probe were applied for library screening, no matter homogenic BAC or heterogenic BAC, site-specificity is higher than short DNA fragment probes.

对于无法通过小基因组填补间隙的区段，可以本物种BAC克隆直接进行染色体步移，采用抑制重复顺序杂交法能够有效抑制大量存在于植物基因组中的重复顺序的干扰，把假阳性率降低到可以接受的范围，从而避免了用大量指纹分析剔除假阳性克隆。

The holistic design of the system and resolved the designs of software and hardware based on VXI bus are put forward according to the demand of test system. The hardware and software is designed, including the formation and design of hardware system, visual instrument software architecture, program design for instrument driver, high speed real-time data collection. In the dynamic test program, the idea of"software is instrument"was applied to meet the diverse test demands. Last, it is proposed that the VXI test system be developed to more totalization and networked. The basic theory of system identification was introduced briefly at first. The principle of spectrum analysis system identification based on Wiener-Hopf equation was expatiated. and the questions in applying Peusdo Random signal as spectrum analysis methods stimulate signal was studied, The generation principle of the peusdo random signal is researched, the characteristic of peusdo random are analyzed from time domain and frequency domain, the inverse repeat peusdo random signal is brought forward to make up the shortage of ordinary peusdo random signal. And a new chaos genetic algorithmis presented to solve the unprecise problem of the traditional method. In the servo valve dynamic characteristic test system, the spectrum analysis methods was applied successfully, the servo valve dynamic characteristic could be acquired rapidly and exactly, the test results were analyzed and the ameliorate methods were brought forward at last.

结合航天某集团液压测试中心测试系统的需求，提出了基于VXI总线的液压测试系统的整体设计方案，完成了测试系统的硬件设计与软件设计，包括硬件系统构成与设计、虚拟仪器软件结构、仪器驱动程序设计、高速同步实时数据采集等内容；以伺服阀动态特性测试为例设计了测试模块，该模块体现了VXI总线仪器软件开发所倡导的&虚拟仪器&的思想，以软件的灵活应用满足了不同的测试需求；结合自动测试系统的发展，研究了VXI测试系统与网络技术结合，实现各种软硬件资源共享，进一步提高设备及数据的利用率和系统的扩展性，使自动测试向综合化、网络化方向发展；研究了伪随机信号作为谱分析法辨识的激励信号时存在的问题，讨论了伪随机信号的产生机理，从频域和时域分析了伪随机信号的性质及其应用，提出了用逆重复伪随机信号弥补了普通伪随机信号的不足，并以混沌遗传算法对逆重复伪随机信号产生过程中的参数进行优化，可以更合理而不是凭经验产生需要的逆重复伪随机信号。

Compared with 30 to 40 percent of the words for those who memorized by repetition.

相比之下，用重复法记忆生词的人只记住了30%—40%。

The data source based on the second soil survey in year of 1982 and repeated soil sampling in year of 2003, and the methods for sampling, determining and analyzing of SOC are auger hole method, K_2Cr_2O_7 oxidation method, area-weigh method respectively, and micro-pipette method is used to determine soil texture....

SOC数据来源于1982年土壤普查和2003年重复采样，土样的采集、分析和统计方法分别为土钻取土法、重铬酸钾氧化法、吸管法和面积加权法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。