重复子

Other 10 GHgenes of vertebrate sepcies downloaded from GenBank hadnt the repeat while the barramundi(L.calcarifer) had one partial repeat. 6. Polymorphic detections were done among three wild populations from S.chuatsi, S.

从GenBank数据库下载的其余十一个物种GH基因中，除尖吻鲈GH基因在第三内含子末端具有该序列的不完全重复外，4种鲈形目鱼类及6种非鲈形目脊椎动物GH基因均缺少该重复序列。

Gene mutations were detected in 25 patients and a 18-year-old girl among 16 families. Trinucleotide repeats of CAG were 73～79. The fragments of abnormal alleles were 380～402bp, and all patients were heterozygous. The copy numbers of normal alleles were 18～40, fragments from 202～270bp. SCV reduction was much obvious compared to MCV, MCV and SCV in lower limb ?ere much more slow than that in upper's. BAEP, VEP were also delayed in latency. The anticipation in parental sex bias were much more obvioius than that in matental's. Cerebellar ataxia was most severe, the next were dysarthria and bulging eyes. Amytrophy was seen only in bed ridden patients. Cerebellar atrophy was more severe than brain stem, cord atrophy was n't observed in all MJD.

结果检出10个家系25例病人及1例症状前18岁女孩有MJD基因突变，CAG三核苷酸重复73～79次，异常等位基因片段长380～402bp，均为杂合子；正常人CAG三核苷酸重复18～40次，等位片段长200～270bp，电生理发现MJD的SCV减慢比MCV明显，而下肢的MCV、SCV又较上肢明显，BAEP、VEP均有不同程度的潜伏期延长或波的异常；MJD的父亲遗传早于母亲，进展也较块，临床以小脑性共济失调为突出症状，其次为构音障碍、突眼等，肌肉萎缩仅见于晚期病人；MRI示小脑萎缩较明显，脑干萎缩并不严重，未见明显的颈髓萎缩。

2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.

根据dystrophin基因5个多态位点（44、45、49和50号内含子以及5'端启动子区的序列）设计引物，在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾：CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身，经高分辨率的变性聚丙烯酰胺凝胶电泳后，利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置，进行扫描分析，确定产物片段的大小。

Based on the elliptic curve cryptosystem, a multi-key sharing scheme in which the Lagrange interpolation polynomial is used and the cheaters can be detected is presented.

文章利用拉格朗日插值的思想，提出了一种基于椭圆曲线的可防欺诈的动态多密钥共享方案；方案中每个参与者的子密钥可以不受限制的重复使用，因此在更新主密钥的时候，无需更改参与者的子密钥，从而减少了可信中心与参与者之间的通信量；方案可灵活地增删参与者，且安全性是基于椭圆曲线离散对数问题的难解性，因而其安全性比在有限域上更高；方案实现过程中解决了检验子密钥真伪的问题。

The rate of repetitive sequences was 2.55% and no SINEs, LINEs, LTR anti-transcription elements or DNA transposon elements were found, although one11 microsatellite was found. 2 In the P1 promotor region, sequence homology between the Tibetan sheep and mouflon, goat, cattle and European bison was 99.7%, 94.2%, 85.9% and 86.5%, respectively, while that for exon 1A was 99.0%, 97.0%, 92.7% and 94.6%, respectively. 3 The molecular phylogenetic tree among these species, constructed by the neighborhood joining method based on the sequences of no-coding region of the GHR genes, placed the two Bovinae species on one branch and the three Caprinae species on the other.

在该非编码序列中，重复序列所占比率为2.55%，不存在SINEs、LINEs、LTR类反转录元件和DNA转座子元件，而发现存在一11微卫星位点；(2)在启动子P1区，藏绵羊与摩弗伦羊、山羊、普通牛、欧洲野牛各物种间同源性大小分别为99.7%、94.2%、85.9%、86.5%；而在外显子1A区段，藏绵羊与摩弗伦羊、山羊、普通牛、欧洲野牛各物种间同源性大小分别为 99.0%、97.0%、92.7%、94.6%。

First, the proposed scheme uses contourlet transform to extract multidirectional and multiscale texture information, and then obtains the feature points invariant to the affine transformation from the low and middle directional subbands by Harris-Affine detector. Second, the feature regions are adaptively computed by the feature scale of the local structure and normalized by U transform. The watermarking is embedded adaptively in those characteristic regions which are normalized. Finally, by vector quantization, several copies of the watermark are embedded into the no overlapped local feature regions in different directional subbands.

首先，用Contourlet变换提取出多尺度、多方向的纹理信息；再用Harris-Affine检测算子从变换域的中、低频方向子带中提取出仿射不变特征点，结合自适应局部结构的特征尺度确定特征区域，并用U变换对其归一化处理，水印就自适应地嵌入到归一化后的区域中；水印嵌入采纳矢量量化的策略，将水印信息重复嵌入到不同方向子带、多个不相交的局部仿射不变特征区域。

The Reduplicate Sub-region Boundary Element Method is used to simulate the full failure process of heterogeneous brittle material since the lattice model with the same geometric size is used to discretize the specimen.

针对非均匀脆性材料格子模型的特点，采用重复子域边界元法模拟了二维非均匀脆性材料的破坏过程。

TPH gene contains a chort of variants, these polymorphisms distribute promoter upstream, intonlB、 1C、7、8and 9, in addition to a microsatellite repeat at 3' downstream.

TPH基因存在多个变异点且具有遗传多态性，这些多态位点分别位于上游启动区、内含子1B区、1C区、内含子7、内含子8及内含子9区，在下游3'区亦有一微卫星重复序列。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

In the interspersed repeats (SINEs, LINEs, LTR elements, DNA elements), SINEs and LINEs occur most frequently, LTR elements occur less and DNA elements occur the least frequently.

在散布重复序列中，短散布重复序列和长散布重复序列出现的最多；长末端重复序列出现的较少，而DNA elements出现的最少。散布重复序列在大鼠和小鼠内含子中分别占插入序列总长度的33%和35%；散布重复序列在大鼠和小鼠内含子中分别占缺失序列总长度的18%和15%。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。