重复妊娠

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证明。

Results: 58 cases of repeated ectopic pregnancy conservative treatment was successful in all, including 39 cases of a course of chemotherapy MTX blood β-HCG dropped to normal, 4 cases of carbuncle of the two MTX treatment were recovered and seven cases of the first two courses United mifepristone treatment success.

结果：58例重复异位妊娠保守治疗全部成功，其中，39例1个疗程MTX化疗血β-HCG下降至正常，4例2个疗程MTX化疗痊愈，7例第2个疗程联合米非司酮治疗成功。

objective to explore the correlation factors of repeated homonymy fallopian tube gravidity, and raise the ideal method avoiding re-onset.

目的：探讨同侧输卵管重复妊娠发病的相关因素，提出避免再发病的理想治疗方式。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

Single factor design was adopted and 12 Yorkshire sows, which were littermate or semi-littermate with similar body weight(initially 250 Kg of mean body weight) at the similar gestation day(86 day), were randomly allotted to 3 treatments (active dry yeast, Se-enriched yeast, control), there were four replicates with one sow per replicate in one treatment.

实验采用单向分类设计，选用血缘一致、体重接近、妊娠日期(妊娠第86至88天)相近的第5胎约克经产母猪12头，随机分为3个处理，每个处理4个重复，每个重复1头猪，分别为：对照组、硒酵母组、活酵母组。

Ninety-one cases of repeat ectopic pregnancy were demonstrated from 1380 cases of ectopic pregnancy.result: the repeat ectopic pregnancy was 6.59%(91/1380) after operation, eighty-four cases demonstrated as tubal pregnancy, six cases were tubal pregnancy three times, seven cases were cured by conservative medication,the recurrence of repeat ectopic pregnancy of twice was 86.81%(79/91). after salpingectomy of the patients who occurred the another tubal pregnancy was 90.41%.

结果：1380例异位妊娠发生重复异位妊娠91例（占6.59%），84例经手术探查证实为输卵管妊娠， 6例为输卵管妊娠3次，7例为药物保守治疗治愈，86.81%重复异位妊娠为连续两次异位妊娠。90.41%输卵管切除者的重复异位妊娠发生在对侧，而保守治疗者72.22%发生在原患侧，两者有显著性差异（p.001）。

P＜0.01. Conclusion Conservative treat-ment and inflamed salpinx are risk factors of recurrent ectopic pregnancy. Shanghai Med

保守性治疗与输卵管炎症是重复异位妊娠的危险因素。

P＜0.05. Only 33.3%of recurrent ec-topic pregnancy occurred on the non-macroscopic inflammatory salpinx.

输卵管有肉眼炎症表现者，84.9％的病例重复异位妊娠发生在炎症侧，而重复异位妊娠发生在无肉眼炎症侧者仅为33.3％(P＜0.01)。

The diagnosis of EP is certain at this time if an intrauterine pregnancy is not observed on TVS and the serum beta-hCG concentration is increasing or plateaued.

下一步是要在两天后重复进行经阴道超声检查及HCG测定。如果经阴道超声仍未见宫内妊娠及HCG浓度增加或居高不下，即可诊断异位妊娠。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。