释放

The nitric oxide released process of nitrosyl metalloporphyrins in vitro was detected with diazotization assay at room temperature, the four nitrosyl metalloporphyrinsbCo-NO、bFe-NO、a_(Co-NO、a_were good nitric oxide donors, the nitric oxide released efficiency is attained about seventy percent.

在室温下采用重氮化反应研究了这些金属卟啉-NO 配合物在水溶液中释放NO的过程，其中四种金属卟啉(bCo-NO、bFe-NO、aCo-NO、aFe-NO)有较好的释放效果，释放率达到近70%。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The effect of media pH, the composition of the outer shell and the manipulation on pulsatile release was also studied.

通过正交设计和体外释放度实验，考察释放介质的pH、处方及工艺对盐酸地尔硫脉冲释放的影响。

In the third chapter of this thesis, a SOTs which could differentially release model drug gliclazide from two drug layers was designed and prepared, such SOTs could release model drug gliclazide at relative faster rate during the first few hours while release gliclazide at relative lower rate in the following hours.

本文的第三章中，设计并制备了一种新型的异步释放格列齐特三层渗透泵片，该三层渗透泵片能在初期几个小时内以较快的速率释放模型药物，而在随后的时间里缓慢的释放模型药物。

The 20 hair at the beginning of the exemple diagnose the method 2 model dextrose of profess to convinced of DM patient go ahead of the rest is able to bear or endure the quantity experiments , morrow or tertian add before doing OGTT take Nai of RG2mg , the insulin that determines to set calm time to nod each and blood sugar level, observe the difference of result of earnings of method of 2 kinds of experiments and correlation, the ratio that compares the Ins between 2 kinds of experiments to release net rise in value of net rise in value of peak value, Ins and blood sugar of △ I/△, Ins releases times rise in value and Ins release the area below the curve .

方法20例初发诊断的2型DM患者先行口服葡萄糖耐量试验，次日或隔日在做OGTT前加服RG2mg，测定各设定时间点的胰岛素和血糖水平，观察2种试验方法所得结果的差异和关联，比较2种试验间Ins释放峰值、Ins净增值和血糖净增值的比值、Ins释放倍增值以及Ins释放曲线下面积。

The pattern of IBU in vitro release from the PCEC matrix obeys the model of zero order dynamics, and the release mechanism is diffusion.

考察了共聚物对布洛芬的体外释放行为，在载药量为5-20％时，药物都能持续且稳定地释放，遵循零级释放动力学，而且主要依照扩散的机理进行。

The results show that the global N\-2O emission per year is about 14.7 TgN\-2O\|N, in which 57% and 43% from natural and anthropogenic sources respectively, 92% from N biogeochemistry and only 8% from N abiology, 70% from soil\|related sources, and about 20% attributable to agricultural soil.

得出全球N2 O年释放总量约 1 4 。7TgN2 O -N ，其中自然源和人为源分别占 57%和 4 3 %。年释放总量中N的生物地球化学过程约贡献92 %、非生物作用过程仅贡献 8%；与土壤有关的释放源约贡献 70 %、农业土壤贡献2 0 %。

We obtain the conditions of asymptotical stability of the pest-eradication periodic solution and permanence of the system.

在第四章中，应用天敌助增(即天敌的人工繁殖和释放)的方法，建立了在固定时刻释放天敌的具有一般功能性反应的三种群的食物链模型，并系统地研究了系统在释放天敌这种外在的不连续受迫作用下的动力学性质，得到了害虫根除周期解存在性和渐近稳定性，以及系统持续生存的条件。4。

Bacterially triggered CDDS 细菌触发型细菌触发型 CDDS The presence of azo reductase enzymes play pivotal role in the release of drug from azo bond prodrugs while glycosidase activity of the colonic microflora is responsible for liberation of drugs from glycosidic prodrugs.

偶氮还原酶溶解酶的存在在偶氮键药释放过程中发挥关键作用，结肠菌群的糖苷酶活性负偶氮还原酶溶解酶的存在在偶氮键药释放过程中发挥关键作用，结肠菌群的糖苷酶活性负溶解释放过程中发挥关键作用的糖苷酶活性糖苷药物的解放。

The main results were as follows:'Brumal jujube' with higher respiration rate, which decreased slightly after harvest, and lower ethylene production, which had not the appearance of respiration and ethylene production peak, ethephon with different concentrations all increased respiration rate and the respiration rate increased with the raise of concentration ,which suggested 'brumal jujube ' was respiratory non-climactic fruits.

结果如下：冬枣采后果实呼吸强度高并呈平缓下降趋势，而乙烯释放量较低无明显高峰出现；不同浓度的乙烯利处理增加了果实呼吸强度，处理浓度越大，果实呼吸速率越高，说明冬枣为非跃变型果实；1000μL.L~(-1)乙烯利和100mg.L~(-1)ABA处理可显著提高绿熟冬枣采后的呼吸强度，两者无明显差异，但乙烯利处理显著增加了乙烯释放量、ACC含量和ACO活性，其作用比ABA明显；GA处理抑制了果实呼吸强度和乙烯释放量的增加。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。