采集用囊

One-day-old SPF chicklings were inoculated via intracephalic injection at a dose of 0.03ml per chickling, consisting of 1:100 tissuer virus suspension. And 4 of experimental group and 2 of control group were killed at 1, 3, 5, 10, 14, 20, 25 and 30 days postinfection, respectively. All the sera, the lymphocyte suspension of peripheral blood, thymus, Fabricius bursa and spleen, were collected.

本研究用AEV-NH937株组织毒1：100倍稀释，0.03ml/只脑内接种1日龄SPF雏鸡，分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只，对照观察组2只，分离血清，制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液，同时采集脑组织，10%福尔马林固定，制备石蜡切片，做H.E。

The purpose of this study, on the base of former research, was to reveal and better understand some molecular pathogenesis of AE, to set up a working model which could be generally used by anther researcher in the field of animal molecular pathology. One-day-old SPF chicklings were inoculated via intracephalic injection at a dose of 0.03ml per chickling, consisting of 1:100 tissuer virus suspension.

本研究用AEV-NH937株组织毒1：100倍稀释，0.03ml/只脑内接种1日龄SPF雏鸡，分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只，对照观察组2只，分离血清，制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液，同时采集脑组织，10%福尔马林固定，制备石蜡切片，做H.E。

And 4 of experimental group and 2 of control group were killed at 1, 3, 5, 10, 14, 20, 25 and 30 days postinfection, respectively. All the sera, the lymphocyte suspension of peripheral blood, thymus, Fabricius bursa and spleen, were collected.

本研究用AEV-NH937株组织毒1∶100倍稀释，0.03ml／只脑内接种1日龄SPF雏鸡，分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只，对照观察组2只，分离血清，制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液，同时采集脑组织，10％福尔马林固定，制备石蜡切片，做H.E。

Using single oocyst infected techniques, three strains of Eimeria tenella, PT0705, FQ0709 and LJ0711, were obtained from classically symptomic coccidian chicken in Putian, Fuqing and Lianjiang Couties, Fujian Province. In order to compare their toxicity against chicken, healthy chicken of 14th-day and 21th-day were artificially infected with 1×104, 5×104 and 10×104 coccidian oocysts of PT0705, FQ0709 and LJ0711, respectively. The results showed that the strain PT0705 was the most toxic to tested chicken based on the clinical symptom, survival rate, weight increment and lesion grade investigations.

采用单卵囊分离技术，从福建省莆田市、福清市和连江县等地鸡场采集的病鸡盲肠内容物样品中，分离获得3株(PT0705、FQ0709、LJ0711)柔嫩艾美耳球虫纯种；为比较这三种虫株对雏鸡的毒性，分别用1×104、5×104和10×104个孢子化卵囊的剂量感染14日龄和21日龄健康无球虫感染雏鸡，通过临床表现、死亡率、增重情况、病变计分等指标比较，发现PT0705虫株呈现最强毒性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。