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Armillaria luteo-virens is a ectomycorrhizal fungus symbiotic with some species of Kobresia in Tibet Plateau, China. A strain was recently isolated from fruitbody tissue of the fungus. To determine whether the strain is the pure culture of the fungus, the internal transcribed spacer region and the first intergenic spacer region (IGS-1) of the nuclear ribosomal DNA of fruiting body and the isolated strain were sequenced and compared. Further, the 5.8S/ITS and IGS-1 sequences of the species were BLAST matched with GenBank database on homology and a phylogenetic tree was constructed based on 5.8S/ITS sequence and two phylogenetic trees were constructed separately based on two IGS-1 sequences.

首次从采自青藏高原、与高原牧草嵩草属Kobresia草本植物形成外生菌根的黄绿蜜环菌Armillaria luteo-virens子实体中分离获得一组织分离菌株,运用rDNA-ITS和rDNA-IGS-1测序技术对该组织分离菌株是否为黄绿蜜环菌的纯培养菌种进行分子鉴定,并基于黄绿蜜环菌的5.8S/ITS和IGS-1序列进行核酸序列数据库GenBank同源性检索比对、构建系统发育树。

In order to investigate whether fragments of Sugarcane bacilliform virus were integrated into Saccharum inter-specific hybrids genome, a DNA fragment homologous to SCBV was amplified from a hybrid sugarcane plant Saccharum inter-specific hybrids var.

为研究甘蔗杆状病毒Sugarcane bacilliform virus的基因组片段是否整合进杂种甘蔗Saccharum inter-specific hybrids基因组,以采自广西甘蔗研究所的1株杂种甘蔗植株叶片总DNA为模板,根据已报道的甘蔗杆状病毒基因组序列设计多条特异引物。

From Cacalia deltophylla (collected from Luqu County, Gansu Province), 22 compounds were isolated, of which 15 compounds have been identified. Among them, two compounds are new. They are named as 3-methyl-4-hydro-8-acetyl-furanonaphth-9-one and 1hydroxy-2-methoxy-3-acetyl-4, 8-dimethyl-5, 8-dihydro-naphthalene.

采自甘肃省碌曲县的三角叶蟹甲草全草的甲醇提取物的石油醚部分和乙酸乙酯部分中,采用同样方法分离得到22个化合物,鉴定了其中的15个化合物,其中有2个为新化合物,分别命名为3-甲基-4-氢-8-乙酰基-呋喃萘-9-酮(2)和1-羟基-2-甲氧基-3-乙酰基-4,8-二甲基-5,8-二氢-萘(3)。

Twenty-three isolates of gray leaf spot pathogen, Cercospora zeae-maydis, collected from growing area of Northern China were clustered based on their soluble protein and isozyme profiles obtained through native PAGE in order to base physiologically the pathogen differentiation at protein or isozyme level.

利用聚丙烯酰胺凝胶电泳技术,对采自北方玉米主产区的23个玉米灰斑病菌菌株进行可溶性蛋白质和同工酶电泳图谱分析及聚类分析,从蛋白质和酶学的多态性水平上分析玉米灰斑病菌的生理分化特征。

The pathogenicity types of Cercospora zeae-maydis were difficultly identified only according to lesion reaction types, thus disease rank and frequency of each one appearing on differential hosts should be most useful for accurate identification.

通过病级评价可将采自北方玉米产区的23个菌株分成5个致病类型。

Excitation-Emission Matrix Fluorescence Spectroscopy was employed to study fluorescence characteristics of chromophoric dissolved organic matter readily released from sediment particles during episodes of resuspension, and bottom waters and surface sediment pore waters were compared in all corresponding sites.

采自厦门湾九龙江入海河口的4个沉积物样品进行了室内再悬浮模拟实验,利用荧光激发-发射矩阵光谱研究了再悬浮过程中从沉积物中释放出的有色溶解有机物的荧光特征,同时通过与相应站位沉积物间隙水和底层水的对比分析,探讨了河口近岸海域的沉积物再悬浮作用作为水体中溶解有机物来源之一的可能性。

Ten compounds were isolated from Cirsium henryi (collected from Zhang County, Gansu Province), structures of 7 compounds have been identified.

采自甘肃漳县的刺苞蓟全草的甲醇提取物中,采用同样方法分离纯化得到10个化合物,鉴定了其中7个化合物的结构,未发现新化合物。

The results of RAPD analysis were consisted with their phenotypic characters very well, which indicated that the RAPD technology could be used to analyze the relationships of some intraspecies and similar interspecies of Coccomyces efficiently.

用筛选出的重复性好、条带清晰的14个随机引物分别对采自安徽黄山和天堂寨的12个齿裂菌属菌株进行了RAPD分析,其结果与表型特征表现出良好的一致性,从而表明RAPD技术可以较好地应用于齿裂菌属种内和近似种间亲缘关系的分析。

The isolate B9 collected from Jilin province in north China, was the most sensitive to high temperature among the 5 isolates, and only 20%of the conidia of this isolate germinated when treated at 40℃ for 10 min.

采自非洲的菌株M189对高温的抵抗力最强,在40℃处理4小时和50℃处理1小时,孢子的发芽率保持在40%以上,60℃处理20分钟仍有大约1%的发芽率。

The germination characteristics of Cupressus chenggiana S.

通过对采自四川小金县的岷江柏CupressuschenggianaS.Y。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。