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Methods The rat model of middle cerebral artery ischemia 2h/reperfusion 22h was established.The neurological scale, cerebral infracted volume, cerebral water content, activities of NOS and SOD were measured. ResultsThe average neurological scote,cerebral infracted volume,cerebral water content,activity of NOS,content of MDA in Naokangling Capsule group significantly decreased.

采用大鼠大脑中动脉缺血2 h/再灌注22 h模型,神经病学评分,脑梗塞范围及脑组织水含量变化,观察脑康灵胶囊抗脑缺血/再灌注损伤的效应;通过测定大鼠脑组织中一氧化氮合酶(nitric oxide synthase,NOS),超氧化物歧化酶(superoxide disumtase,SOD)活性及丙二醛(malondialdehyde,MDA)含量的变化以探讨药物作用的机制。

MethodsThe rat model of middle cerebral artery ischemia 2h/reperfusion 22h was established.The neurological scale, cerebral infracted volume, cerebral water content, activities of NOS and SOD were measured. ResultsThe average neurological scote,cerebral infracted volume,cerebral water content,activity of NOS,content of MDA in Naokangling Capsule group significantly decreased.

方法采用大鼠大脑中动脉缺血2 h/再灌注22 h模型,神经病学评分,脑梗塞范围及脑组织水含量变化,观察脑康灵胶囊抗脑缺血/再灌注损伤的效应;通过测定大鼠脑组织中一氧化氮合酶(nitric oxide synthase,NOS),超氧化物歧化酶(superoxide disumtase,SOD)活性及丙二醛(malondialdehyde,MDA)含量的变化以探讨药物作用的机制。

The results of this study are summarized below.1 A variety of additives such as proteins,glyoxal / glycerol,alcohols,metal salts,as well as ethylene monomer were used in this study and all of them could improve the leaching resistance of the three kinds of borate(boric acid,borax,disodium octaborate tetrahydrate) in different extents through variety forms.

本研究的结果归纳如下:(1)本研究中所使用的多种助剂如蛋白质、乙二醛/丙三醇、醇类化合物、金属盐以及乙烯单体等都能通过各种形式,不同程度的提高三种硼酸盐(硼酸、硼砂、四水合八硼酸二钠)的抗流失性,但效果均不是特别明显。

And try to find out the effect of TSA on the proliferation and differentiation of rat tracheal stem cell. Methods 1.Preparation of 5-Fu Model and TSA treatment Two weeks" Wistar rats provided by the animal center of the China Medical University. The animals were sacrificed by 10% Chloral Hydrate 0.4ml/100g. Then the tracheas were excised aseptically. Then they were douched with PBS and cultured in 1:1 mix of Dulbecco"s modified Eagle"s medium and Ham"s F-12 medium (DMEM/F12) containing 120 mg/ml 5-Fu and 10% fetal bovine serum for 12h at 37°C. The tissues were then refreshed by culturing in DMEM/F12 containing 10% FBS and 200nM TSA.

1、制备气管损伤模型及剥离上皮取约200克左右的Wistar大鼠,雌雄不限(中国医科大学实验动物中心提供),腹腔注射10%水合氯醛0.4ml/100g,无菌条件下取出气管,无菌PBS反复冲洗,洗净血液和粘液,置于DMEM/F12培养液中(含10%胎牛血清),于培养液中加入终浓度为100mg/ml的5-Fu,37℃,5%CO2孵育12小时,弃去上述培养液,换成新鲜DMEM/F12液(含10%胎牛血清),同时加入终浓度为200nM的TSA继续培养,于换液后3、6、12、24、48小时分别10%中性福尔马林固定,石蜡包埋,制成4μm厚的组织切片。

A comparison of the efficacy and safety of chloral hydrate versus inhaled anesthesia for sedating infants and toddlers for transthoracic echocardiograms.

水合氯醛与吸入麻醉对于婴幼儿经胸超声心动镇静检查的有效性和安全性。

Blood gas ; Horse ; Jing-Song-Ling ; Chloral hydrate ; Laser

血气分析;马;静松灵;水合氯醛;激光

The effects of different doses of urethane,chloral hydrate and pentobarbital sodium on the electrophysiological characteristic of sciatic nerve of toad in vitro were investigated by means of the extracellular recording action potential.

使用BL-420生物机能实验系统,采用细胞外记录神经干动作电位的方法,观察了氨基甲酸乙酯、水合氯醛和戊巴比妥钠3种麻醉剂对蟾蜍离体坐骨神经电生理特性的影响。

Bioleaching process with At.f can leach 0.282ppm of solubility mercury in 25 days. After the rats taking orally 5 days (total mercury ≤0.2μg), it was found to have synergistic interaction with pentobarbital sodium and chloral hydrate in sedative activity depending on the concentration of total mercury.

实验显示氧化亚铁硫杆菌对朱砂具有浸出作用;同时实验显示:服用朱砂浸出液5天后(服用汞总量≤0.2μg),小鼠各组织器官内未检测出汞,但朱砂浸出液却对小鼠水合氯醛和戊巴比妥钠睡眠催眠有明显促进作用,并且呈现剂量依赖关系。

The 4th left rib was cut and a model of left coronary artery occlusion/release was carried outo Rats were divided randomly into 4 groups: control group without LCA occlusion (group A, n=6);I/R group(group B, n=6); ketamine(5mg.kg-1)+I/R group (group C, n=6); ketamine(10mg.kg-1)+I/R group(group D,n=6)o All rats in groups B, C , D were subjected to 30 minutes of LCA occlusion followed by 120minutes of reperfusion. Rats in group C and D were injected with 5mg.kg-1 and 10mg.kg-1 of ketamine before reperfusion, respectively. Significant electrocardiogram and color changes at the area at risk were considered indicative of successful coronary occlusion and reperfusionc Serum was exampled from left jugular vein at 30 minutes and 120 minutes during reperfusion to measure IL-6 and TNF- level by ELISA measurements. After reperfusion of 120 minutes, the heart was removed and the cardiac apex was exampled with snap-frozen in liquid nitrogen and stored at -70 C .

采用左冠状动脉前降支结扎开放建立心肌缺血/再灌注模型,健康SD大鼠24只,随机分为心包打开假手术组(A组,n=6),缺血/再灌注对照组(B组,n=6),5 mg·kg~(-1)氯胺酮+缺血/再灌注组C组,n=6,缺血30min后右腹股静脉注入5 mg·kg~(-1氯胺酮,10mg·kg~(-1)KTM+I/R组D组,n=6,缺血30min后右腹股静脉注入10 mg·kg~(-1氯胺酮。10%水合氯醛40 mg·kg~(-1)腹腔注射麻醉,气管切开,连接多功能监护仪记录心电图;小动物呼吸机人工呼吸,呼吸频率60次/分,潮气量2ml/100g,于左胸第四肋间打开胸腔暴露心脏,在左心耳下1mm左冠状动脉处,用丝线,眼科外用不锈钢小圆针穿过心肌浅层,稳定10min后将U型含有铜丝的胶管置于冠状动脉表面一起结扎(A组不结扎,B、C、D组结扎);结扎开始左心室心尖部即由红色变暗,30 min后呈暗红色,心电图中出现S-T段抬高,说明缺血形成。

Chaper 3: Aldol condensations of aromatic aldehydes with ketones under solvent-free condition to synthesize α,β-unsaturated ketones in good to excellent yields using PEG400 and powdered anhydrous K2CO3 as catalysts at 90℃or 120℃were described.

第三章:在无溶剂条件下,由PEG400 和无水K2CO3催化芳香醛与酮的Aldol 缩合反应合成α,β?不饱和酮,得到较高的收率。

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