酸酪蛋白

Here we show that phenylarsine oxide and diamide (both were inhibitors for protein tyrosine phosphatases), but not genistein (an inhibitor for protein tyrosine kinases), adenosine, wortmannin and LY294002 (all were inhibitors for phosphatidylinositol 3- and 4-kinases), could inhibit P-selectin exocytosis on activated platelets and could abolish the P-selectin mediated aggregation of activated platelets to neutrophils.

我们发现蛋白酪氨酸磷酸酶的抑制剂phenylarsine oxide和diamide能够抑制P-选凝素从活化的血小板中外排出来，并能够抑制P-选凝素介导的活化的血小板在白细胞表面的粘附，而蛋白酪氨酸激酶的抑制剂genistein，磷脂酰肌醇3-或4-激酶的抑制剂adenosine、wortmannin和LY294002在这些实验中没有作用。

Here we show that phenylarsine oxide and diamide (both were inhibitors for protein tyrosine phosphatases), but not genistein (an inhibitor for protein tyrosine kinases), adenosine, wortmannin and LY294002 (all were inhibitors for phosphatidylinositol 3-and 4-kinases), could inhibit P-selectin exocytosis on activated platelets and could abolish the Pselectin mediated aggregation of activated platelets to neutrophils.

我们发现蛋白酪氨酸磷酸酶的抑制剂phenylarsine oxide和diamide能够抑制P-选凝素从活化的血小板中外排出来，并能够抑制P-选凝素介导的活化的血小板在白细胞表面的粘附，而蛋白酪氨酸激酶的抑制剂genistein，磷脂酰肌醇3-或4-激酶的抑制剂adenosine、wortmannin和LY294002在这些实验中没有作用。

In the protein of scolopendra, scorpio, pheretima,the content of Glycine, Alanine, Valine,Tyrosine,Phenylalanine and Arginie is higher,but the content of glutaminic acid is significantly lower than the daily animal and plant albumens.

探讨结果如下：一、氨基酸分布与蜈蚣、全蝎、地龙药理作用的关系在蜈蚣、全蝎、地龙三种天然动物药材中甘氨酸、丙氨酸、酪氨酸、苯丙氨酸、精氨酸含量高，谷氨酸含量则显著低于常见动植物蛋白。

Studies also indicated that genistein exsists not only in soybeans but also in many kinds of plants such as vegetables and fruits.As a potential anticancer agent, the actional mechanisms of genistein mainly includes as follows:First, genistein can depress the activity of protein tyrosine kinase and transduction pathways for the phosphorylation of receptors and mitosis signal. So genistein can lead to cells' proliferation depressed. Second, genistein has minimal effects of phytoestrogens. It can be combined with estrogen receptor and improve the synthesis of cellular sex hormone binding glulobin, and improve the activity of UDP-glucuronyl transferase. Through these pathways, it can inhibit the cell activity of breast cancer and prostate cancer.

作为一个很有潜力的抗肿瘤物质，三羟异黄酮的作用机制主要包括：①抑制蛋白酪氨酸激酶活性，可阻抑PTK引起的受体磷酸化和有丝分裂的信号传递，导致癌细胞增殖受抑；②弱雌激素作用：可通过与体内雌激素受体结合，并可增加细胞内性激素结合球蛋白的合成，增加UDP-葡糖醛酸转移酶的活性等途径抑制乳腺癌和前列腺癌细胞活性；③拓扑酶Ⅰ和Ⅱ抑制剂，抑制细胞活性；④上调细胞周期性负性调节因子P21WAF1/CIP1的表达，使之负性调节因子作用增强；⑤可阻止胰岛素样生长因子-Ⅰ、肝细胞生长因子和神经生长因子的作用而抑制肿瘤生长；⑥其他：抗氧化作用、抑制热休克蛋白、诱导细胞凋亡、抑制新生血管生成和抑制多种耐药相关蛋白等。

Water,caprylic/capric triglyceride,mineral oil,butylene glycol,stearic acid,acetylated lanolin,glycerin,ethylhexyl methoxycinnamate,dimethicone,polyisoprene,triethanolamine,cyclopentasiloxane,butyl methoxydibenzoylmethane,titanium dioxide,persea gratissima oil unsaponifiables,sodium DNA,borago officinalis seed oil,oenothera biennis oil,simmondsia chinensisseed oil,hydrolyzed RNA,tocopheryl acetate,urea,hydrolyzed extensin,retinyl palmitate,sodium hyaluronate,helianthus annuusseed oil,tocopherol,ascorbyl palmitate,ascorbic acid,lanolin alcohol,c12-20 acid peg-8 ester,cyclotetrasiloxane,glyceryl stearate,dimethiconol,ceteareth-20,carbomer,magnesium aluminum silicate,propylene glycol,peg-8,silica,BHT,citric acid,phenoxyethanol,methylparaben,butylparaben,ethylparaben,isobutylparaben,propylparaben,chlorhexidine digluconate

水，辛酸/癸酸三酸甘油酯，矿物油，丁二醇，硬脂酸，乙醯化羊毛脂，甘油，4-甲氧基肉桂酸-2-乙基己基酯，地美司康，聚异戊烯，三乙醇胺，环戊硅氧烷，丁基甲氧基二苯甲醯基甲烷，二氧化钛，酪梨油，DNA 钠盐，琉璃苣種子植物油，月见草油，荷荷巴籽油，水解RNA，醋酸盐维他命E，尿素，水解伸展蛋白，维他命A酯，透明质酸钠，葵花籽油，维生素E，抗坏血酸棕榈酸酯，维生素C衍生物，羊毛脂醇，C12-20 酸 PEG-8酯，环四硅氧烷，甘油硬脂酸，聚二甲基硅氧烷醇，鲸蜡硬脂醇醚-20，卡波姆，硅酸镁铝，丙二醇，聚乙二醇-8，二氧化硅，二丁基羟基甲苯，柠檬酸，苯氧乙醇，羟苯甲酯，羟苯丁酯，羟苯乙酯，羟苯甲酸异丁酯，羟苯丙酯，洗必泰葡萄糖酸盐

The effect of genistein (0.8 mg/kg) was partially inhibited by L-NAME, or by sodium orthovanadate (50 μg/kg), a potent inhibitor of protein tyrosine phosphatase;(2) geni...

GST的这一效应可被L 硝基精氨酸甲酯部分阻断，预先注射蛋白酪氨酸磷酸酶抑制剂正钒酸钠( 5 0 μg/kg)，可部分抑制GST( 0 8mg/kg)引起的效应；( 2 )向肾血管灌注环路中直接注射GST也可剂量依赖性地降低肾动脉的灌流压，预先注射正钒酸钠可完全抑制GST引起的效应，而L NAME对此效应没有影响；( 3 )肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压，这一效应可被正钒酸钠部分抑制，而L NAME对此无影响。

Our data are showed that apoptosis parameters including DNA fragmentation, nuclear morphological change and sub-G1 fraction, appeared after 24 h by 5 μM rottlerin treatment. Furthermore, rottlerin caused a decrease in 72% of ODC protein expression and an increase in threonine, rather than serine and tyrosine dephosphorylation on ODC enzyme compared with the control after incubation for 10 h. To determine whether ODC could down-regulate rottlerin-induced apoptosis, HL60 cells were stably transfected with ODC cDNA. HL60-ODC cells exhibited a 2.5-fold increase in ODC protein compared with vector only (HL60-pcDNA3) or HL60 cells.

在我们筛选天然化合物实验中，发现rottlerin抑制鸟胺酸去羧化活性最具成效，之前的研究人员已知蛋白激Cd调节鸟胺酸去羧化的活性，因此在人类白血病HL60细胞处理rottlerin的实验中发现加入rottlerin二十四小时后，细胞进行计划性死亡，并可侦测到DNA片段、细胞核浓缩及sub-G1提增；而细胞处理药物十小时后与控制组细胞比较，观察到鸟胺酸去羧化的蛋白表现与活性下降50﹪以上；除此以外，测定鸟胺酸去羧化去磷酸化的情况，发现於酥胺酸位置有显著差异，而丝胺酸和酪胺酸位置并无差异。

Extracellular-regulated kinase 3(ERK3) is an atypical member of ERKs, lacking the threonine and tyrosine residues in the activation loop, carrying a unique C-terminal extension and being mainly regulated by its own protein stability.

其活化环不同于一般的ERK，缺少苏氨酸和酪氨酸残基，拥有一段独特的C端延伸序列。ERK3蛋白是一个不稳定的蛋白，它的生物学活性主要依靠其蛋白稳定性和激酶活性调节。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

Western blot was used to examine the phosphorylation of NR2A in rat hippocampus. Results Both Src and Fyn antisense ODNs could decrease the phosphorylation of NR2A induced by cerebral ischemia/reperfusion, while administration of Src antisense ODNs had more suppressive effect on the phosphorylation of NR2A than Fyn antisense ODNs.

对NR2亚基有磷酸化调节作用的是Src家族酪氨酸蛋白激酶（Src family of protein tyrosine kinase， Src PTKs），这是一类膜结合的非受体酪氨酸蛋白激酶；现已知Src家族成员中Src、Fyn、Yes、Lyn、Lck在中枢神经系统中均有表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。