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The bulky ortho-brominated aryl groups are implanted to create nonplanarity around amino center. Thus, the effective conjugation length to the protonation site is shortened and the energy gap between neutral and protonated forms is narrowed. The protonation can only occur at the nitrogen atom of the benzothiazole and the temperature variation disturbs the equilibrium between the neutral and monocation and the reverible thermochromic effect can thus be achieved.

在邻位的位置引入立体阻碍大的溴原子,进而控制质子化过程的酸碱平衡只发生在 benzothiazole 上的氮原子上,企图达到只有中性分子,与单阳离子两种稳定的形式存在,经由外界的刺激下,例如温度或酸的量的改变,促使逆反应的发生,而达到热致变色的效果。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识,会用显微镜检测观察微生物,培养基的制备、消毒与灭菌,分离与纯化微生物,细菌的计数法具备分析化学的理论知识,能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理,了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识,PAGE凝胶电泳技术,高速离心掌握基因工程的基本理论知识,基因的提取和纯化,PCR技术原理,基因重组技术掌握酶的基本理论知识,酶的固定化方法,酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器:天平(台天平,电子天平,分析天平)、酸度计、分光光度计、液气相色谱仪,酸碱滴定仪器,显微镜,离心机,超声波清洗器、恒温振荡培养箱、超净工作台,蒸气灭菌锅、恒温水浴锅,PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。

Mondell engineering stone is high-technical decorating surface which is isomerized and polymerized from picked natural rimed stone ( the conpozation of Sio2 is over 99.99 percent). The stone is glittering, hard. It is not easy to fade or change the shape. It is not radiant and acid-fast. It is antibacterial and clean. It has good physical and chemic performance. This series is wildly used for its beautiful decoration in walls, floor, platform and so on. It combines features of natural stone and PMMA solid surface to get the rich and glittery colour.

蒙代尔工程石是采用精选的天然石结晶矿产为原料(石英砂Sio2的含量达到99.99%以上),在全自动生产线的高温高压状态下异构聚合而成的高科技装饰面材,具有晶莹剔透、硬度高、不变形、不退色、无辐射性、耐酸碱、易清洁、抗菌、卫生等优异的物理性能及化学性能,应用空间相当广泛,可应用于地面、外墙、台面等装饰自然美:真正的石材质感、兼顾天然石和亚克力人造石二者之优点,色彩丰富、晶莹剔透。

This survival time was not significantly altered by bilateral denervation of cervical vagi (the major sensory system for detecting changes in pulmonary conditions), by bilateral denervation of peripheral arterial chemoreceptors (the major sensory system for detecting changes in arterial blood gases and pH), by pretreatment with dimethylthiourea, or by pretreatment with milrinone (a phosphodiesterase Ⅲ inhibitor that may avoid respiratory muscle fatigue).

而截断双侧颈部迷走神经(负责侦测肺内部情况的最主要感觉神经)、摘除双侧的周边化学接受器(负责侦测动脉血液气体及酸碱值的最主要化学接受器)、或投予Milrinone(一种phosphodiesteraseⅢ抑制剂,藉抑制cAMP被分解,以增加细胞内cAMP的量,可以使疲乏的呼吸肌内再增加收缩力)、Dimethylthiourea等药物的动物,都无法延长其存活时间。

The application, proposed hydrotalcite-like compounds as a new type of environmentally friendly solid catalysts in the aldol condensation, nitrile hydrolysis, epoxide ring-opening reaction of the oxidation of aromatic compounds, alcohols oxidation, reduction of nitro compounds, and many other important reactions in organic synthesis has an important application potential, and with little pollution compared to conventional catalysts, easy separation of advantages of high reactivity.

叙述了水滑石类化合物作为固体酸碱催化剂、氧化还原反应催化剂、催化剂载体等方面的应用情况,提出了水滑石类化合物作为一类新型的环境友好的固体催化剂,在醇醛缩合、腈类水解、环氧化物的开环反应、芳烃类化合物的氧化、醇类氧化、硝基化合物的还原等许多重要的有机合成反应具有重要的应用前景,并且与传统催化剂相比具有污染小、易分离、反应活性高等优点。

Methods The ionization constant was determined with acid-basle titration in acetone/water solutions with different ratios, the pH at half neutralization point was regarded as pKa, the value to infinite diluted in pure water was explored and the lgP was determined with bottle-shaking method by HPLC.

方法采用酸碱滴定法测定各不同体积比的丙酮-水溶液中药物半中和点时的pH值作为药物在该体积比的丙酮-水溶液中的pKa值,再外推至纯水中药物浓度无限稀释时的pKa值;采用HPLC配合摇瓶法测定药物的分配系数。

No weld plastic storage tanks, motor transport tank, tank transport ship products using the international advanced one-time model of rotating plastic molding process, the product has a total of polyethylene forming a seamless, impact resistance, anti-aging, weight Light, non-leakage, acid, long life, consistent with the health standards, etc., the use of the performance is much better than the traditional glass containers steel, PVC welding containers, polypropylene containers welded, steel-lined rubber, steel-lined steel containers such as glass, ordinary life is a traditional more than twice the FRP, GRP impact is more than three times, to a large extent can be replaced by stainless steel, titanium and other m e t al containers, is the best of today's new generation of high-performance corrosion-resistant chemical liquid storage transportation equipment, and cheap.

无焊缝塑料储罐、汽车运输贮罐、船舶运输贮槽系列产品采用国际先进的旋塑工艺一次性模式成型,产品具有整体聚乙烯一次成型无接缝、抗冲击、抗老化、重量轻、不渗漏、耐酸碱、寿命长、符合卫生标准等优点,使用性能大大优于传统的普通玻璃钢容器、聚氯乙烯焊接容器、聚丙烯焊接容器、钢衬橡胶、钢衬玻璃钢等容器,使用寿命是传统普通玻璃钢的二倍以上,抗冲击是玻璃钢的三倍以上,在相当大的范围内可取代不锈钢、钛等金属制品容器,是当今最理想的新一代高效的化工液体耐腐蚀贮运设备,且价廉物美。

This series of products adopt international advanced one-time model of rotating plastic molding process, the product has a total polyethylene forming a seamless, impact resistance, anti-aging, light weight, non-leakage, acid, long life, consistent with the health standards advantages, the use of the performance is much better than the traditional glass containers steel, PVC welding containers, polypropylene containers welded, steel-lined rubber, steel-lined steel containers such as glass, ordinary life is a traditional glass fiber reinforced plastic for more than twice, impact glass steel more than tripled, to a large extent can be replaced by stainless steel, titanium and other m e t al containers, is the best of today's new generation of high-performance liquid chemical storage and transportation equipment, corrosion resistance, and good value for money.

本系列产品采用国际先进的旋塑工艺一次性模式成型,产品具有整体聚乙烯一次成型无接缝、抗冲击、抗老化、重量轻、不渗漏、耐酸碱、寿命长、符合卫生标准等优点,使用性能大大优于传统的普通玻璃钢容器、聚氯乙烯焊接容器、聚丙烯焊接容器、钢衬橡胶、钢衬玻璃钢等容器,使用寿命是传统普通玻璃钢的二倍以上,抗冲击是玻璃钢的三倍以上,在相当大的范围内可取代不锈钢、钛等金属制品容器,是当今最理想的新一代高效的化工液体耐腐蚀贮运设备,且价廉物美。

In basic properties of silver grass, the basic biology construction, fiber morphology, buffering capacity , surface wet ability and longitudinal tensile strength was mainly studied; in manufacturing process of SPB, the effect of board density and UF addition on physical and mechanical properties of SPB was mainly discussed, and By means of test of orthogonal experimental design and searching test, the relationship between moisture content , hot-pressing temperature , water-proofing agent addition and the TS of SPB was studied in detail. Through the study of the test production process of SPB, the best technological conditions were determined.

在荻草基本性质方面,主要研究了荻草的基本生物构造、纤维形态、化学成分、酸碱缓冲容量以及荻草的表面润湿性和纵向抗拉强度;在荻草刨花板制造工艺方面,主要讨论了不同密度、施胶量与板材物理力学性能之间的关系,并通过正交试验的方法深入研究了热压温度、板坯含水率以及防水剂施胶量对荻草刨花板吸水厚度膨胀率的影响,通过研究确定了较佳的试验生产荻草刨花板的工艺条件。

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Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象,运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查,调查结果发现,石家庄市初中生物教师这一群体普遍存在职业倦怠,情感枯竭程度偏高,成就感偏低,去个性化程度最为严重。

In measurements of a day,generallyspeaking,the photosynthesis of birch in mesophytic habitat is better than that in xerophytichabitat(peak values are 12.8,10.33μmolCO2m-2s-1 respectively);that of sexual birch inmesophytic habitat is better than that of clone birch(peak values are 9.87,6.71μmolCO2m-2s-1respectively);that of young tree is better than that of seedling(peak values are12.37,10.05μmolCO2m-2s-1 respectively).

在一天中的各个时刻,总体说来,中生生境生长的白桦光合作用超过旱生生境生长的白桦光合作用(净光合速率峰值分别为12.8、10.33μmolCO2m-2s-1);白桦幼树的光合作用超过白桦幼苗(净光合速率峰值分别为12.37、10.05μmolCO2m-2s-1);中生生境有性白桦的光合作用超过无性白桦的光合作用(净光合速率峰值分别为9.87μmolCO2m-2s-1、6.71μmolCO2m-2s-1)。