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Preparation of ratiometric pH nanosensors and its appication in intracellular pH detection of the anti-tumor drugs treated Hela cells The ratiometric pH nanosensors that contained a pH sensitive indicator fluorescein isothiocyanate and a reference dye tris(2, 2′-bipyidyl) dichlororuthenium hexahydrate had been prepared with O/W microemulsion method.

利用该内参比pH纳米传感器尺寸小可以通过细胞的内吞而无损伤地进入细胞内的优势,进一步结合流式细胞学分析方法,对不同抗肿瘤药物作用的Hela细胞内的pH变化进行了分析,结果表明:不同抗肿瘤药物作用后的Hela细胞内pH的变化情况并不一样,有的作用后引起了细胞酸化,有的作用后引起了细胞碱化,有的作用后基本上不引起变化。

The synthesis of the pentacyclic skeleton of Et-743 and the construction of the five chiral centers thereof.1. The core pentacyclic skeleton of phthalascidin was constructed with L-dopa as the starting material via thirteen steps. First, two basic synthons, the C-1 functionalized tetrahydroisoquinoline compounds 8 and N-protected Z-dopa derivative 9, were prepared from L-dopa. They were coupled via amide bond to form the dipeptide compounds 10, which were the first key intermediates. Then the primary hydroxyl groups were transformed into the corresponding amino aldehydes 11 via Swern oxidation. Followed by the intramolecular Pictet-Spengler cyclization, the pentacyclic compounds 12 were synthesized.

五环骨架的构建及五个正确手性中心的确立:1.1我们以L-多巴为起始原料,经过多步官能团保护和转化合成了基本结构单元8然后和苯丙氨酸衍生物9,通过缩合反应生成酰胺中间体10,经Swern氧化得到醛化合物11,然后再次利用分子内改良的Pictet-Spengler反应一步构建c,d两环,得到五环骨架化合物12,最后,实现了21-位羰基向氰基的转化,得到了C-1位官能化的化合物14,化合物14不但具有和Et-743一致的五环骨架,而且分子中五个手性中心立体构型也与其完全相同:其中,3,位和13,位的手性中心由原料L-多巴带入,而1,位,11,位和21,位手性中心则通过不对称诱导而确立。

Hyphae, loaded with CTC under pH 6. 8 condition and then grown in pH 8. 0 medium for a little while to destroy the apical acidification, could gave very diffuse fluorescence images of CTC membrame bound Ca〓 and vermiform fluorescence spots due to mitochondrias under pH 6. 8 no longer were clearly distinquished, which implied that besides mitochondrias, other cellular organelles with Ca〓 also were stained with CTC. Thus mitochondrias are not the only one intracellular Ca〓 storage, endoplasmic reticulums and Golgi bodies in the same zone may be also the Ca〓 storages. But the extreme apical zone under 2μm of the growing hyphal tip was still almost devoid of stain under pH 8. 0.The CTC fluorescence was concentrated in the subapic zone beyond about 2μm from the tip and then gradually became lower behind about 40μm from the tip. These results did not support the hypothesis which suggested that the cell wall vesicles in the extreme apical zone were intracellular Ca〓 storages.

将菌丝在pH6.8条件下负载CTC,再置于pH8.0培养介质中短暂生长一会儿以消散菌丝顶端的酸化区域,则CTC膜结合Ca〓呈现弥散的荧光影像,在pH 6.8培养条件下显示的蠕虫状的线粒体荧光斑点不再能够清晰辨认,说明除了线粒体之外,还有其它含Ca〓细胞器,也被CTC染色,线粒体并不是细胞内唯一Ca〓库,还可能包括内质网、高尔基体等细胞器,但菌丝最顶端2μm以前的细胞壁泡囊区域不能被染色,最大荧光强度仍位于菌丝顶端2μm以后区域,约45μm以后荧光变弱,实验结果不支持细胞壁泡囊为菌丝胞内Ca〓库的假设。

Possible fluorescence enhancement mechanism is also studied. Chapter 3, a novel assay of DNA is proposed based on the measurement of enhanced resonance light-scattering signals resulting from the interaction of nano-CdS-Lcysteine with DNA.

3第三章,用L-半胱氨酸作为修饰剂合成了功能化的CdS量子点,并利用DNA的加入对其共振光散射光谱的影响建立了超灵敏测定DNA的方法。

One fear is that the increasing acidity will kill off pteropods and similar creatures with calcium-carbonate shells or skeletons.

人们对此的一个担忧就是越来越强烈的酸化会杀死翼足目和其他类似的具有碳酸钙外壳或骨骼的生物。

By studying effects of the amount of NaOH, CS2 and dissolving water and xanthation conditions on the viscose quality, the optimal technology of viscose-making was obtained. By studying the effects of H2SO4 concentration in coagulating bath, NaOH concentration in desulfurating bath and plastifier content on the physical strength of mulch sheet, the optimal sheet forming technology was found out.

通过研究制备粘胶时NaOH用量、CS2用量、溶解用水量以及黄酸化条件对粘胶品质的影响,确定制备粘胶的最佳工艺;通过研究成膜过程中凝固浴H2SO4浓度、脱硫浴NaOH浓度、增塑剂用量等对薄膜物理强度的影响,确定了成膜的最佳工艺。

Tissue acidosis is a dominant phenomenon under the physiological or the pathological conditions, such as synapse activities, ischemia or epilepsy and so on.

组织酸化是机体中较为常见的一种变化。正常的神经元活动或缺血、癫痫发作等病理条件的刺激均可以降低细胞外的pH。

The comprehensive electroplating wastewater from a large-scale electroplating industrial area in Shenzhen has been studied. Its treatment method has been investigated. It is ascertained that acidification-oxidation decomplexation and chemical precipitation of hydroxide and sulphide process are used. The best decomplexation agent chosen is bleaching liquid NaClO. The operational parameters are as follows: dosage of bleaching liquid 1.2 mL/L wastewater, reaction pH 2.3, and reaction time 2 h. Under these conditions, the requirements of the discharge standard of electroplating wastewater can be met effectively by adjusting the pH and adding sodium sulphide, overcoming the deficiencies of traditional processes of electroplating wastewater treatment.

以深圳某大型电镀工业园产生的综合电镀废水为研究对象,对其处理方法进行了探索,确定了酸化-氧化破络反应及氢氧化物和硫化物共沉淀的处理方法;通过实验选择漂水为最佳破络剂,最佳破络操作参数为漂水用量1.2 mL/L,反应pH为2.3,反应时间2.0 h,在此基础上调节pH并加入硫化钠即可实现电镀废水的达标排放,克服了传统电镀废水处理工艺的不足。

Environmental Monitoring Station of Wuhu City, 241000, Wuhu, Anhui, China Abstract: Acidrain is one of the most serious environmental poll ution problems ,To resolve its chemicalcomposition and its rule of seasonal change will be hel pful in researching the cause ofacid rain and the method of acidification contr ol .

芜湖市降水化学成分特征及变化趋势的初步研究王小月张书贵摘要:酸雨是我国严重的环境题目之一,对其化学成份及其四季变化规律的分析、研究将有助于酸雨成因的探讨和酸化控制的研究。

It would be a mistake to think of geo-engineering as a substitute for curbing carbon-dioxide emissions—not merely because of the acidification of the oceans, but also because if you ever stop fertilising the oceans or spraying the atmosphere or whatever, the problem will rapidly return.

认为生物工程可以作为控制二氧化碳排放的替代品的观点是错误的,这不仅因为海洋的酸化,更因为当停止给海洋施放铁物质或者停止向大气层喷洒海水或者停止其他什么方式,原来的问题会迅速再次出现。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。