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The citric acid-producing strain Aspergillus niger 2363-2, was isolated and used in our research. The fermentation conditions were optimized as follows: sucrose as carbon source 8. 0-14. 0%, ammonium chloride as nitrogen source 3. 0-4. 0g/l, initial pH 2. 0, and cultural temperature 30℃. The influential factors of formation of spores were also studied, and a series of measures were taken to restrain the spore formation on the basis of our experimental results. With the consideration of the cultural environment and microbial cell itself, the factors affecting the duration of citric acid-producing activity of microbial cells were examined, and it is found that undissociated citric acid and the ageing of microbial cells are the main factors. The experimental results also show that partial replacement of fermentation broth can lengthen the duration of citric acid -producing acitivity of microbial cells.

筛选出了用于试验研究的柠檬酸发酵用菌Aspergillus niger 2363-2,确定了其最适发酵条件—碳源选用蔗糖,浓度8.0~14.0%,氮源为NH〓Cl,浓度3.0~4.0g/l,发酵初始pH值为2.0,培养温度30℃;讨论了分生孢子产生的影响因素,并通过试验提出了抑制分生孢子产生的措施;从分析微生物所处的环境和微生物本身两个方面出发,探讨了影响微生物菌体产酸活性持久性的因素,认为分子态柠檬酸和菌体本身的老化是影响微生物产酸活性持久性的两个主要因素,并提出采用部分置换发酵液发酵能延长微生物菌体的产酸活性。

On the basis of the former results, Miscibility of unseparated reaction solution with varied resins were investigated by means of co-solvent method and the glossiness of the film method. The miscibility of 344# acrylic resin with the mixture was better than others, when the ratio of the mixture to 344# acrylic resin was one to ten, the miscibility attained best, and the glossiness of the film was 100 percent.The effect of miscibility and molecular weight on delaminating rate were investigated by the co-solvent method.

在聚马来酸醉溶解性能、相容性测定的基础上,通过共溶剂法、溶液涂膜法和溶解度参数法,考察了反应液不分离直接和不同树脂复配的情况。344#短油度醇酸树脂和接枝物的相容性较好,在接枝物:短油度醇酸树脂=1:10时,相容性达到最好,涂膜光泽值达到100%。

The characteristic of ABR is change water power characteristic and to improve the term of mass transfer in the reactor in order to increase the reaction velocity; and increase alkalinity to improve the capability of abirritation of the system through add Na2CO3 to reaction and a optimal pH condition which fit to acidic bacterium come into being. In result, the organic matters are removed efficiently in system.

异波折板水解酸化反应装置最大的特点是改变了水流特征,改善反应装置内酸化菌与基质之间的传质条件,以加快反应速率:同时在反应装置内加入碳酸钠,碳酸钠溶液作用在于提高反应装置内废水的碱度,增强系统的缓冲能力,把pH值控制在适合酸化菌生长的范围,充分发挥微生物的活性,使系统的有机去除能力大大提高。

Ion exchanage materials are also used to purify the chlorous acid and chlorine dioxide solutions, without causing degradation of said solutions, to exchange undesirable ions in the chlorous acid and chlorine dioxide solutions with desirable ions, such as stabilizing ions, and to adjust the pH of chlorous acid and chlorine dioxide solutions.

所述离子交换材料也可以用于纯化亚氯酸和二氧化氯溶液,从而在亚氯酸和二氧化氯溶液中用想要的离子如稳定离子交换不想要的离子,并调节亚氯酸和二氧化氯溶液的pH值,而不造成所述溶液的质量降低。

This article microbiofilmtechonology fermention colibacillosis ASP-311 technology, to the strains to fumaric to slfia to fermetation asparate; qsdhmm oluv in movingbed emulsifications, decolourization decolourization of temperature, time, as well as the pH value of l - omniberaing conditionson decolouring asparate, the resulting effect of: movingbed or by 1.0% concentration, temperature is 60 700℃ decolourization, 0.5h, decolourization time for the pH of 6, in this condition, oluv of 3.2% absorbance at decolouring.

毕业论文翻译(本文生物学术语多,切勿使用在线翻译)本文利用微生物发酵培养技术,以大肠杆菌ASP-311为发酵菌株,以富马酸为底物来发酵生产天冬氨酸;研究了发酵液中活性炭的使用量、脱色的温度、脱色的时间以及pH值等各种条件对L-天冬氨酸脱色效果的影响,最终得出:活性炭的浓度为1.0%,脱色温度为60℃,脱色时间为0.5h,pH为6,在此条件下,发酵液的吸光度为3.2%,此时的脱色效果达到最佳。

Using microorganism fermentation cultivation technique, with escherichia coli strains of ASP - 311 for fermentation, with rich fumaric acid as the substrate to ferment produce l-aspartic acid, Study the result of activated carbon, and the temperature, the use of bleaching time and discoloring pH value of various conditions, aspartic acid decoloring effect, and finally: the concentration of carbon, decoloring temperature of 1.0% 60 degrees, the bleaching time for 0.5 h, pH for 6, in this condition, the result is the absorbency of decolour 3.2%, to achieve the best effect.

本文利用微生物发酵培养技术,以大肠杆菌ASP-311为发酵菌株,以富马酸为底物来发酵生产天冬氨酸;研究了发酵液中活性炭的使用量、脱色的温度、脱色的时间以及pH值等各种条件对L-天冬氨酸脱色效果的影响,最终得出:活性炭的浓度为1.0%,脱色温度为60℃,脱色时间为0.5h,pH为6,在此条件下,发酵液的吸光度为3.2%,此时的脱色效果达到最佳。

Specificity studies on the substrate revealed that the HdPO was capable of oxidizing not only the o-diphenol, but also tyrosine. The apparent Km value on tyrosine was about 9.52 mmol/L. As shown in the experiments, HdPO behaved oxidizing activities both on diphenol and monophenol, which implies that the HdPO was probably not only a type of tyrosinase-type PO but also a catechol-type.

底物特异性研究还发现此HdPO不仅对邻苯二酚具有氧化活性,对酪氨酸底物也具有氧化活性,且对酪氨酸的Km值约为9.52mmol/L,说明该酶既具有酪氨酸酶活性,又具有儿茶酚酶活性。

The white low-density powder of the the sodium salt of polyaspartic acid was got by dropping the hydrolyzable solution to wiscked high speedly carbinol which Consumption amount is about 3-6 times to the amount of solution, the powders density is about 0.35g/ml.4 Polyaspartic acid was characterized by NMR and IR.5 The scale inhibition behavior of polyaspartic acid was studied and result shows: The calcium carbonate inhibition rate is up to 99% at the dosages of 12.5mg/ Land when pH value is 9.0, Ca2+ concentration is 120mg/L and HCO3- concentration is 366mg/L.6 The primary design was put forward for industrialized production of PASP.

在此工艺下得到聚天冬氨酸钠粉术,外观为白色的轻质粉术,堆积密度0.35g/ml,不用进行脱色和粉碎。 4。用红外光谱和核磁共振谱图对聚天冬氨酸进行了表征。 5。对合成的聚天冬氨酸钠对CaCO_3的阻垢性能进行了研究。结果表明:在Ca~(2+)离子浓度120mg/L(以碳酸钙计300mg/L),HCO_3~-离子浓度为366mg/L(以碳酸钙计600mgL),PH值=9.0的条件下,阻垢剂浓度达到12.5mg/L时阻垢率可达99%。

The results indicated that the effect of pH on adsorption of theanine was not obvious within range of 6~11. The adsorption date fit well into the Freundlich and Langmuir isotherm equation within temperature range of 303~323 K and investigative concentration range. The isosteric enthalpy of adsorption, free energy and entropy of adsorption showed that adsorption of theanine was endothermic and spontaneous.

结果表明:在pH6~11时,pH值对717阴离子交换树脂对茶氨酸吸附量影响不大;在温度303~323K和茶氨酸质量浓度范围内,717阴离子交换树脂对茶氨酸吸附行为符合Freundlich和Langmuir吸附等温方程;热力学参数(吸附焓、自由能、吸附熵)表明此吸附过程是吸热和自发的。

For different mixed ferment time, Sensory assessing was not different obviously;pH value,DM,CP,NDF,ADF content were all katabatic or decreasing with extending of mixed silage time; NH3-N/tN%and lactic acid ,acetic acid, propionc acid and butyric acid concentrations were increased gradually,butproportion of lactic acid was the most in total acids.

2不同发酵时间的感官评定结果无明显差别;pH值、DM、CP、NDF和ADF随发酵时间延长均逐渐降低或减少;NH3-N/tN%、乳酸、乙酸、丙酸和丁酸均逐渐增加,但以乳酸占总酸比例最大。

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