酶蛋白

Construction of yeast that can directly utilize starch by introducing amylolytic enzyme genes into Saccharomyces cerevisiae is of great commercial importance in brewing industry and ethanol production.

酵母是一种重要的工业生产菌，广泛应用于乙醇、酒精饮料和单细胞蛋白的生产，但口前使用的酵母菌种如酿酒酵母等因缺乏水解淀粉的酶，不能直接利用淀粉。

Rats in LPS group (n = 12) were intratrachealy administered 200 μg of LPS dissolved in 100 μl saline after anesthetization, rats in control group(n = 12 ) were given intratracheal 100 μl saline.

通过检测LPS组大鼠气管注入LPS后第2、4、7天肺组织炎症指标包括肺炎症积分、支气管肺泡灌洗液中性粒细胞计数、巨噬细胞计数、TNF-α、IL-1β及IL-8含量和气道黏液分泌指标包括肺组织阿先蓝—过碘酸雪夫阳染面积、免疫组化黏蛋白Muc5ac及逆转录-聚合酶链反应Muc5ac mRNA的表达，对LPS诱发大鼠肺炎症和气道黏液高分泌的改变进行动态观察。

In order to study functions of glutathione S-transferase theta during preparation and synthesis of fibroin in Antheraea pernyi, expression of GSTT in silk glands of the fifth instar larvae was analyzed.

为明确柞蚕谷胱甘肽硫转移酶－theta在柞蚕丝素蛋白准备和合成过程中所起的作用，采用生物信息学的方法克隆了柞蚕GSTT基因，并分析其在5龄幼虫丝腺中的表达规律。

Then the content of the interleukin (IL-1) in serum were measured by the method of enzyme-linked assay; From the HE dyeing, the change of morph in the arthrodial was observed by the light microscope; From the AB-PAS dyeing, the change of PG in the cartilage matrix was observed; By all of that the therapeutic efficacy of the medicine wine Yong GuKangNing to the disease of osteoarthritis was estimated, and the mechanisms to the disease was also investigated.

用酶免法测定血清中白细胞介素(IL-1)的含量；常规HE染色，光镜下观察关节软骨结构形态学变化；AB-PAS染色，光镜下观察软骨基质中蛋白聚糖的染色变化；评价永康宁骨康宁对膝骨性关节炎的治疗效果，并探讨其作用机制。

Coli. The bacterially expressed SPE-16 also demonstrated ribonuclease-like activity in vitro.

活性实验显示，天然和重组的SPE16蛋白都具有明显的类核糖核酸酶活性。

Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells.

对北京协和医院根据细菌学或／和组织学诊断的肺外结核患者31例，应用酶联免疫斑点技术检测6kD早期分泌靶向抗原和10kD培养滤过蛋白肽段库刺激后外周血中释放γ-干扰素的特异性T细胞。

Methods To determine the serum PCT of 35 patients with bacteriosis and 15 patients with nonbacterial disease by gold immunochromatographic assay,and determine C-reactive protein and alkaline phosphatase of neutrophilic granulocyte in corresponding period.

方法采用半定量的胶体金免疫结合法，测定35例细菌感染性疾病住院患者及15例非细菌感染性疾病住院患者的PCT，并同期测定患者C-反应蛋白与中性粒细胞碱性磷酸酶。

In this paper, the cloning, expression and phylogenetic analysis of AmphiSDHD and AmphiS27 from the gut cDNA library of amphioxus Branchiostoma becheri tisingtauense are carried out.AmphiSDHD, encoding the cytochrome b small subunit in mitochondrial succinate-ubiquinone oxidoreductase, was isolated from the gut cDNA library of amphioxus Branchiostoma belcheri tsingtauense.

本文从青岛文昌鱼(Branchiostoma belcheri tsingtaunese)肠cDNA文库中，分离得到克隆L095和094，经鉴定它们分别编码文昌鱼线粒体琥珀酸-泛醌氧化还原酶中细胞色素b的小亚基和核糖体蛋白S27(AmphiS27)。

Bis-(2,3-dibromo-4,5-dihydroxy-phenyl)-methane is a natural bromophenol with significant inhibition against PTP1B IC_(50=2.4μmol/L, which was isolated from the ethanol extract of red algae Rhodomela confervoides.

双-(2，3-二溴-4，5-二羟基苯基)-甲烷是从松节藻醇提物中分离鉴定出的溴酚类化合物，体外活性筛选发现，它具有极强的蛋白酪氨酸磷酸酶1B(PTP1B)抑制活性IC_(50=2.4μmol/L。

Change in the concentration of endogenous IAA was measured with enzyme-linked immunosorbent assay, and the distribution of IAA-binding protein in the protoplasts in the cambial region was tested with an immuno-fluorescence technique, being improved by the authors, during the cambial active period in Broussonetia papyrifera L.

构树（Broussonetia papyriferaVent。）形成层活动周期中用酶联免疫吸附法测定了形成层区域的内源IAA浓度的变化，并用自行发展的免疫荧光检测方法测定了由此处生活细胞制得的原生质体中IAA结合蛋白的分布。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。