酶蛋白

At the end of treatment ,the differences in the behavior science were observed ; the change of the nasal mucosa secretion were inspected cross-reference after build model; IL-2,IL-4mRNA in the spleen were used to do revers transcriptive polymerase chain reaction.

用卵清蛋白致敏大鼠制成变应性鼻炎动物模型，辛芷鼻敏胶囊及阳性对照组药物鼻炎康灌胃给药，观察其行为学差异，造模前后分别取鼻粘膜分泌物涂片检查，用逆转录—多聚酶链反应对大鼠脾脏IL-2、IL-4mRNA进行半定量检测。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Ribonuclease as an RNA enzyme existing in mammalian cells has the effect of antitumor in vitro and in vivo.

广泛存在于机体内的核酸酶是一类序列保守，结构稳定，以RNA为底物的蛋白。

This low molecular weight ribonuclease with selective cytotoxicity for tumor cells isolated from oocytes of the Chinese northeast frog will join the candidate proteins of drug molecular for the treatment of cancer.

这种从中国林蛙卵中分离纯化出的具有选择性细胞毒性的小分子量核糖核酸酶，为肿瘤的治疗提供了新的候选蛋白分子。

In this paper,extrusion processing and addition of vitamin E were studied for the stability of rice bran .

采用Sevag法、三氯乙酸法和酶法对米糠多糖进行脱蛋白研究。

Methods The RCAⅠidentifying Terminal Galβ14 GlcNAc glycoprotein was acquired by RCAⅠ(Ricinus communis agglutininⅠ) affinity chromatography method from nor mal person serum and was marked by the horseradish peroxidase.133 tumor patients,64 benign disease patients and 240 normal person serums were detected by the subtraction of agglutinin.

应用RCAⅠ亲和层析法从正常人血清中获得RCAⅠ相关末端Galβ14GlcNAc糖蛋白，用辣根过氧化物酶标记，采用凝集素差减法，对133例肿瘤患者，64例良性疾病患者和240例正常人血清进行检测。

Five nitrogen concentrations including 0 mg/L,150 mg/L,300 mg/L,450 mg/L and 600 mg/L were sprayed on the root of D.styracifolium in field to study the effects of different N concentration on gas exchange,chlorophyll content,soluble protein content and nitrate reductase activity in leaves of seedlings.

采用田间试验的方式，设0mg/L，150mg/L，300mg/L，450mg/L和600mg/L5个N浓度处理，研究其对广金钱草苗期叶片气体交换、叶绿素含量、可溶性蛋白含量和硝酸还原酶活性的影响。

Ai Saiga and colleagues cite previous studies indicating that chicken breast contains collagen proteins with effects similar to ACE inhibitors, mainstay medications for treating high blood pressure.

Ai Saiga和他的同事引用先前的研究指出，鸡胸肉包含胶原蛋白，它和血管紧张素转换酶抑制剂有类似作用，而ACEI是治疗高血压的主干药物。

Influence of concentration, pH, shear rate, NaCl, CaCl2, glycerol, ethanol and heating preservation on the viscosity of pepsin-solubilized collagen from fish scale of grass carp were studied by Rotary Viscometer.

采用旋转流变仪系统考察了浓度、pH、剪切速率、NaCl、CaCl2、丙三醇、乙醇和保温时间对草鱼鱼鳞酶溶性胶原蛋白粘度的影响。

Through this domain,sterols cause Scap and HMG-CoA Ruductase to bind to Insigs,newly characterized membrane proteins of the ER.

通过这个结构域，固醇促进Scap和HMG-CoA还原酶与Insigs的结合，一种新发现确定其结构的内质网膜蛋白。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。