酶蛋白

To construct a retroviral vector carrying immortalizing gene hTERT, screen gene puro, reporter gene EGFP and recombination site sequence LoxP for reversible cell immortalization. Linearized pBABE-puro was cut by restriction enzyme Nhe Ⅰ and ligated with loxP double strands to construct a vector pBABE-puro-lox.

目的 探讨以hTERT作为永生化基因、嘌呤霉素乙酰转移酶基因puro作为筛选基因、加强型绿色荧光蛋白EGFP作为报道基因、loxp作为重组位点构建可回复性永生化逆转录病毒载体，为建立可回复性永生化细胞株莫定基础。

To investigated the relationship of the signal transduction pathway and other signal molucules, three signal transduction inhibitors were tested for their effects on L929 cell and the change of caspase activation. The inhitors include Staurosporine Quinacrine (PLA2 inhibitor) andVerapamil (Ca2+ channel blocker).

为了探讨γTNFβ和TNFβ诱导细胞凋亡的信号转导通路与其它信号分子的关系，我们检测了蛋白激酶C抑制剂Staurosporine、磷脂酶A2抑制剂Quinacrine和钙离子通道阻断剂Verapamil对γTNFβ及TNFβ杀伤L929细胞的影响及其伴随的Caspase8和Caspase3活性的变化。

A quinone compound that serves as an electron carrier between flavoproteins and in cellular respiration.

辅酶 q一种苯醌衍生物，在黄素蛋白中以及细胞呼吸作用为成分携带者

Using SLT SPECTRA reader to determine the level of thrombomodulin.

采用SLT SPECTRA酶标仪测定血浆血栓调节蛋白含量。

And 4.5％ inhibition rate to CCL-187 and HeLa cell receptively using same dose of 5-FC.

这一结果提示ND-1scFv-CD融合蛋白具有一定的酶活性，可将5FC转化为5FU而杀伤细胞，并具明显靶向专一性。

Objective To establish gemcitabine-resistant pancreatic cancer cell strain and study the role of thioredoxin reductase in drug-resistant process.

目的 建立对吉西他宾耐药的胰腺癌细胞株，探讨硫氧还蛋白还原酶在耐药过程中的作用。

The inclusion bodies were solubilized in 50mM Tris-buffer at pH 12.5 and then were diluted to refold the denatured proteins. Refolding of D-hydantoinase by direct and fed-batch operations was investigated . Furthermore, the influence of the additives, CTAB and MnCl2, for soluble protein recovery was also studied.

实验中先以pH 12.5， 50mM Tris-bufferru将包涵体溶解，再藉由稀释法使其复性，并探讨直接稀释法与馈料稀释法对乙内醯尿酶复性的影响，另外探讨添加剂是否对可溶蛋白回收率有所帮助。

To change into curd.A white, tasteless, odorless protein precipitated from milk by rennin.

酪蛋白一种由凝乳酶从牛奶中沉淀出来的白色、无味、无臭的蛋白质。

A piece of such a confection.A white, tasteless, odorless protein precipitated from milk by rennin.

酪蛋白一种由凝乳酶从牛奶中沉淀出来的白色、无味、无臭的蛋白质。

The products had been cloned, restrictively cut aside, and overlappingly amplified to ligate a consecutive sequence of 2547 bp in length that contains the whole HCV 5'NCR and all the structural protein coding regions.

以连接聚合酶链反应将这5个片段拼接为一连续的长2547 nt的片段，含HCV完整的5′NCR和全部的结构蛋白编码序列。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。