酶蛋白

A sandwich ELISA was established taking the antiserum as the primary antibody. The sensitivity of the ELISA was 0.412 ng GH/ml of sample. Inter-assay and intro-assay coefficients of variation were 2. 58％(n=6) and 5. 69％(n=6) respectively. Serial dilutions of serum and pituitary homogenate from orange-spotted grouper yielded dose response curves that were parallel to the standard curve. And the GH of Acanthopagrus butcheri was also parallel to the standard curve. Prolactin of goldfish and recombinant growth hormone of common carp showed no cross-reactivity with the antiserum of orange-spotted grouper GH. It showed that the established RIA was specific and sensitive.

以斜带石斑鱼重组GH抗血清为第一抗体，羊抗兔抗体为第二抗体建立了双抗体夹心酶联免疫测定法，该系统的灵敏度为0.412ng GH／ml(n=6)，组内变异系数为2.58％(n=6)，组间变异系数为5.69％(n=6)，斜带石斑鱼血清和脑垂体稀释液曲线与斜带石斑鱼重组GH蛋白的标准曲线相平行、黑棘鲷GH的系列稀释曲线与标准曲线相平行，金鱼催乳素、重组鲤鱼GH的系列稀释曲线与标准曲线不平行，表明建立的斜带石斑鱼GH ELISA具有良好的特异性和重复性，灵敏度达到了测试血清样品中GH的水平。

Taken together, we concluded this EA at zusanli acupoint significantly can lower plasma glucose levels of normal Wistar rats under ivGTT. Therefore, the action of EA can through parasympathetic system and NO release via lowering the plasma FFA to enhance glucose tolerance.

综合以上，我们的结论是电针足三里能明显提升正常大鼠ivGTT的血糖下降程度，可能透过副交感神经的乙醯胆碱及一氧化氮合成酶降低血中FFA达到较佳降糖作用，而且电针可使胰岛素讯息蛋白IRS-1与nNOS相对含量增加。

It was shown that CpeS is the lyase of Cys-84 ofβ-CPC andβ-PEC.

这些结果表明：藻蓝蛋白裂合酶起到了催化PCB与Cys-84连接的作用。

Therefore,pepsin and Alcalase was suitable to preparate peptides by hydrolyzing wheat protein.

因此，胃蛋白酶和碱性蛋白酶是酶解小麦蛋白制备多肽理想的蛋白酶。

OBJECTIVE: To construct a prokaryotic expression plasmid of the main catalytic domain of dipeptidyl peptidase of murine fibroblast activation protein αand obtain the target fusion protein in order to provide basis for production of antibodies and anti-tumor vaccine targeting FAPα.

目的：构建小鼠成纤维细胞激活蛋白α二肽基肽酶催化核心序列的高效原核表达载体，进行该序列的原核表达、纯化和复性，为下一步高效抗体制作和以FAPα为靶标的抗肿瘤疫苗研发提供实验基础。

Results showed that hemalbumin possessed the peroxidase property, and it was a stable, active molecule.

实验结果表明，血红白蛋白具有过氧化物酶特性，是一稳定而又活泼的分子。

The results show that: after ion implantation, contents of soluble protein and activities of peroxidase in the experimental.

结果表明：离子注入后，减低萝卜过氧化物酶活性和蛋白含量。

Analysis of protein sequence indicated that it has high similarity with glutathione peroxidase from genebank database.

蛋白序列分析表明与基因库谷胱甘肽依赖的过氧化物酶有高度的同源性。

Objective:To investigate the role of protein tyrosine phosphatase 1B(PTP-1B) in pathogenesis of type 2 diabetes.

目的：研究蛋白酪氨酸磷酸酶1B(PTP-1B)在2型糖尿病发病中的作用。

Objective To study the expression of protein tyrosine phosphatase PRL-3 and its relationship with angiogenesis and clinicopathological features of breast cancer.

目的研究蛋白酪氨酸磷酸酶PRL-3在乳腺癌组织中的表达及其与乳腺癌血管形成和临床病理特征之间的关系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。