酶蛋白

Immunohistochemical staining indicated that Nattokinase positive staining grains were found in the brush-border membrane of epithelial cell from duodenum, jejunal to ileal in the Nattokinase extract group, and they showed most predominant in the jejunum, and there were many nut-brown positive staining grains in kytoplasm of epithelial cell.

利用链霉菌抗生物素蛋白-生物素-过氧化物酶复合物(Streptavidin-biotin-peroxidase complex，SABC)免疫组织化学技术，在国内外，首次对兔口服摄入的纳豆激酶进行肠道吸收定位研究。

Enzymatically Hydrolyzed Reduced Minerals Whey Protein Concentrate (from Cow's Milk), Vegetable Oils (Palm Olein, Soy, Coconut and High-=Oleic Safflower or High-Oleic Sunflower), Lactose, Corn Maltodextrin, and less than 1.5% of: Potassium Citrate, Potassium Phosphate, Calcium Chloride, Calcium Phosphate, Sodium Citrate, Magnesium Chloride, Ferrous Sulfate, Zinc Sulfate, Sodium Chloride, Copper Sulfate, Potassium Iodide, Manganese Sulfate, M. Alpina Oil A source of arachidonic acid (ARA, naturally found in breast milk.

酶水解减少矿物乳清浓缩蛋白，植物油（棕榈油，豉油，椰子和高=油酸红花或高油酸葵花籽），乳糖，玉米麦芽糊精，不到1.5 ％的：柠檬酸钾，磷酸钾，氯化钙，磷酸钙，柠檬酸钠，氯化镁，硫酸亚铁，硫酸锌，氯化钠，硫酸铜，碘化钾，硫酸锰，先生高山油（来源的花生四烯酸，自然发现母乳。

Enzymatically Hydrolyzed Reduced Minerals Whey Protein Concentrate (from Cow\'s Milk), Vegetable Oils (Palm Olein, Soy, Coconut and High-=Oleic Safflower or High-Oleic Sunflower), Lactose, Corn Maltodextrin, and less than 1.5% of: Potassium Citrate, Potassium Phosphate, Calcium Chloride, Calcium Phosphate, Sodium Citrate, Magnesium Chloride, Ferrous Sulfate, Zinc Sulfate, Sodium Chloride, Copper Sulfate, Potassium Iodide, Manganese Sulfate, M.

成分Ingredients 酶水解减少矿物乳清浓缩蛋白，植物油（棕榈油，豉油，椰子和高=油酸红花或高油酸葵花籽），乳糖，玉米麦芽糊精，不到1.5 ％的：柠檬酸钾，磷酸钾，氯化钙，磷酸钙，柠檬酸钠，氯化镁，硫酸亚铁，硫酸锌，氯化钠，硫酸铜，碘化钾，硫酸锰，先生高山油（来源的花生四烯酸，自然发现母乳。

Experiment of the third part use the first part"s production as carrier, selecting Geotrich candidum and Candida tropicalis of higher production of protein also selecting producing the cellulose enzyme Bacillus subtilis FS and Lactobacillus bulgaricus by ourselves separated, design orthogonal test and a series of single factor experiment, finally assurancing the inoculation quantity of four strains germ, is Geotrich candidum 5%, Candida tropicalis 5%,Bacillus subtilis FS 5% of FS, Lactobacillus bulgaricus 3%, the most suitable development temperature is 30 "C , the development time is 15 days.

实验的第三部分是以第一步共培养所得的培养物为载体，选择产蛋白量高的白地霉、热带假丝酵母及本室筛选的产纤维素酶芽孢杆菌FS和自行分离的保加利亚乳酸杆菌，设计正交实验和一系列单因素实验，最终确定了四株菌的接种量，分别为白地霉5%，热带假丝酵母5%，FS5%，保加利亚乳杆菌3%，最适培养温度为30℃，培养时间为15天。

AIM: To investigate the effects of selective phosphodiesterase 3 inhibitor olprinone on cough response in guinea pigs sensitized and challenged with ovalbumin.

目的：研究选择性磷酸二酯酶3抑制剂奥普力农对卵蛋白致敏和激发豚鼠咳嗽反应的影响。

Objective:To study the relation between serum ceruloplasmin oxidase activity and tumors in gynecology.

目的：探讨血清铜蓝蛋白氧化酶活性变化与妇科肿瘤的关系。

Experiments were made to study the oxidase gene and its expression.

尝试利用分子生物学和蛋白组学手段对HCCB00304的氧化酶进行研究。

Pocine interferon α mature protein genes were amplified by reverse transcription polymerase chain reaction, and the recombinant replication-defective human adenovius serotype 5 plasmid pAd-poIFN-α was constructed. When the recombinant plasmid pAd-poIFN-α was linearized with PacI, and then transferred into HEK-293A cells, the virus plaque was isolated and purified in HEK-293A cells by three of plaque purification passages.

本研究利用RT-PCR方法扩增猪α干扰素成熟蛋白基因后构建了重组腺病毒质粒pAd-poIFN-α，经PacI酶切后转染HEK-293A细胞，3次噬斑纯化后获得了重组腺病毒rAd-poIFN-α。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Soy protein isolate ; soy peptide ; angiotensin converting enzyme inhibitory activity ; in vitro digestion ; pepsin ; pancreatin

大豆分离蛋白；血管紧张素转化酶抑制剂；体外消化；胃蛋白酶；胰蛋白酶

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。