酶蛋白

Adipo R2 and Krs genes were lowest expressed in EA group. The genes of TNF-αand Utr were lowest expressed in the groups of EN and ST, respectively. This result was coincidence with that of SSH.

对脂联素受体2(Adiponectin recptor 2，AdipoR2)、赖氨酸tRNA合成酶(lysyl-tRNA synthetase，Krs)、肿瘤坏死因子α(tumor necrosis factorα，TNF-α)、Dystrophin相关蛋白(Utrophin，Utr)等4个基因进行定量PCR检测，在不同处理组小鼠肝脏中的表达情况有所不同。

The cells were transfected with the eukaryotic expression vector αS1-LA-psv and the activity of β-galactosidase was detected after transfected from 24 h to 120 h. Human alpha lactalbumin was detected at 72 h after transfected too.

将构建的真核表达载体αS1-LA-psv 转染奶牛乳腺上皮细胞，培养24～120 h均检测到了β-半乳糖苷酶的表达；培养72 h检测到细胞中人α-乳白蛋白的表达，表达量约为

The results indicated that three protease can not hydrolyze β-lactoglobulin uniquely but all the component of whey protein.

本研究还比较了酶解法和离子交换树脂法去除乳清蛋白中β-乳球蛋白的效果。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

Methods: Separate and abstract the effective components of Huangqi (Omni-Saponin、total-flavone) and Danshen (Danshen-ketone、 Danshen-Quinone). Applying L-Arginine methyl ester to pregnant rats by I. P to make Nitrogen Oxide Synthesis blocking models (uterus-placenta-fetus lacking blood and oxygen model or pregnant hypertension syndrome model). On day 12, 18 of pregnancy paunched the rats and took out the fetus and placenta/uterus. Measured the basal BP during pregnant period and BP、protein、basal activity each day. Observed placenta/deciduas ultramicroscopic structure by electron microscope and took count of languishment cells. Measured the expressive intensity of bcl-2、Bax of placental nutrient cells by SABC method.

一氧化氮合成酶阻滞剂L-精氨酸甲酯妊娠大鼠腹腔给药，制作一氧化氮合成阻滞模型（子宫-胎盘-胎儿缺血缺氧或妊娠高血压模型），妊娠第12、18天剖腹取胎、胎盘／子宫，妊娠期测基础血压，每日测血压、蛋白质量、基本生理活动；电镜观察胎盘／蜕膜超微形态结构，并进行细胞凋亡计数；免疫组化检测胎盘滋养细胞bcl-2、Bax表达强度；原位杂交法检测胎盘滋养细胞、血管内皮细胞VEGF、eNOS、iNOS、MCP-1、MMP9（基质金属蛋白酶9）mRNA表达。

Methods The factor Ⅴ Leiden mutation was analyzed in 75 healthy and 118 ICVD patients using PCR-RFLP analysis.

应用聚合酶链反应和限制性片段长度多态性分析方法对国人 118例ICVD患者及 75名健康者进行FⅤLeiden突变检测，86例ICVD患者检测aCL ，2 7例进行抗活化蛋白C检测。

With natural licorice flavone, levorotation VC and collagen protein to efficiently enhance the skin's metabolism, inhibit the melanin by decrease the tyramine oxidase, recovery skin and safely white skin.

蕴含天然植物提取物甘草黄酮，配以左旋维生素 C 、胶原蛋白等安全美白成分，提高肌肤新陈代谢，并通过降低酪氨酸酶的活性，抑制黑色素的产生，从而缓解妊娠期间面色灰暗，色素沉着形成妊娠斑现象，令肌肤恢复往日光彩，适合在产后使用。

Objective To study the relationship between the lipoprotein lipase gene and type 2 diabetes mellitus and its lipid metabolism.

目的：探讨脂蛋白脂酶基因变化与2型糖尿病及其脂质代谢的关系。

Objective To study the construction of recombinant wild lipoprotein lipase gene plasmid and its expression in COS-1 cells.

目的 研究野生型人脂蛋白脂酶cDNA重组表达质粒的构建了及其在COS-1细胞中的表达。

Fluorospectorphotometer was employed to investigate the intracellular concentration of rhodamine123 to reflect the effect of lobeline on the activity of MDR-related protein P-glycoprotein.

多功能酶标仪测定洛贝林干预对细胞内罗丹明123荧光强度以反映其对细胞多药耐药蛋白P-gp活性的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。