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To detect the marks reflecting the liver functions as follows:①The marks to reflect the capability to synthesize proteins, TPROT, ALB, GLB;②The marks to reflect the injury of the liver cells: AST, ALT, CHE;③The marks to reflect the capability to egest the bile, excrect, and detoxicate: TBILI, DBILI, IBILI, TBA;④The enzymes to reflect the cholestasis: GGT, ALP. Results The results of both the morphological and the pathological studies showed that the degree of the liver cirrhosis was of the terminal stage.

肝功能指标:①反映肝细胞蛋白合成代谢功能的指标:总蛋白TPROT,白蛋白ALB,球蛋白GLB;②肝细胞受损的指标;谷草转氨酶AST,谷丙转氨酶ALT,胆碱脂酶CHE;③反映肝脏胆排泄、分泌及解毒功能的指标:总胆红素TBILI,直接胆红素DBILI,间接胆红素IBILI,总胆汁酸TBA;④胆汁淤积指示酶:谷氨酰转肽酶GGT,碱性磷酸酶ALP。

That means Pho85 kinaseand calcineurin were involved in salt tolerance with an identical target protein or in 中科院上海生化与细胞所博士学位论文摘要 the same pathway. Inhibition of calcineurin decrease the YPH499, pho80? mutant,and pap1 (pcl7)? mutant Mn2+ tolerance but not that of pho85? mutant and thepho85? mutant was more sensitive to Mn2+ than YPH499, pho80? mutant, and pap1(pcl7)? mutant even with the addition of cyclosporin A. Therefore, the conclusioncould be drawn that PHO85 gene played a dominant role in Mn2+ homeostasisregulation in compare with calcineurin. As for Ca2+ tolerance, cyclosporin A canincrease the tolerance to Ca2+ of all the mutant mention above, that means Pho85kinase and calcineurin function antagonistically in regulation of Ca2+ homeostasis.In Bioinformatics, BRI3 gene is a novel gene without any function clue but givehigh conservation in mammalian.

我们通过钙调磷酸酶的特异抑制剂环孢菌素A研究了YPH499、+2+pho85缺失株、pho80缺失株、pap1缺失株在钙调磷酸酶失活后对Na、Mn、2+Ca金属离子敏感性的变化,结果显示Pho85蛋白激酶和钙调磷酸酶通过直接+或间接激活同一个靶蛋白或途径来增强细胞对Na的耐受;和钙调磷酸酶相比,2+PHO85的缺失对酵母细胞Mn耐受性的破坏是相对控制性的,钙调磷酸酶的失2+活不能进一步降低pho85缺失株对Mn的耐受能力;Pho85和钙调磷酸酶在对2+Ca的耐受调节中是相互拮抗的,钙调磷酸酶的失活能增加pho85缺失株和2+pho80缺失株的Ca耐受。i中科院上海生化与细胞所博士学位论文摘要对本实验室克隆的人新基因BRI3进行系统的生物信息学分析,发现它是一个在哺乳动物中保守的,但功能未知的基因。

There are experimental results as follows:(1) Eriophorum comosum Nees shows strong cold resistance in plasmalemma permeability research. The lethal temperature of Eriophorum comosum Nees is the lowest LT_(50 in comparison with another two plants and is lower than -11.5℃ in August 2005 and December 2005; Soluble protein content of each plant is rising before or after cold hardiness. By SDS-PAGE, Protein band is analysed. Band quantity of Eriophorum comosum Nees is the most and its band color is darkest.(2) Soluble sugar content of three plants after cold hardiness are higher than before cold hardiness, moreover soluble sugar content after cold hardiness shows a decline and there is a narrow range in Eriophorum comosum Nees; Free Proline accumulation is changed by cold stress, and cold resistance is relative to free Proline accumulation; chlorophyll content of both three plants, including chlorophyll a, chlorophyll b and chlorophyll a/b, show a decline tendency along with the descent of temperature. Cynodon dactylon is highly sensitive to cold stress.(3) The soil POD and urease activities' absolute value of Erioophorum comosum Nees and Pogonatherum panideumHack were more than that of Cynodon dactylon before or after low temperature stress, especially POD activities.

实验结果表明:(1)在膜透性的研究中,丛毛羊胡子草抗寒性最强,采用电导率法测得半致死温度LT_(50最低,丛毛羊胡子草抗寒锻炼前后半致死温度LT_(50均小于-11.5℃;在可溶性蛋白含量研究中,抗寒锻炼前后三种植物的可溶性蛋白含量均增加,同时采用SDS-PAGE法,通过对蛋白条带分析,以丛毛羊胡子草条带数最多且着色深;(2)在可溶性糖的研究中,三种植物经抗寒锻炼后叶片可溶性糖含量都比抗寒锻炼前高,且经过抗寒锻炼后三种植物可溶性糖均呈下降趋势,丛毛羊胡子草可溶性糖含量下降较小;从游离脯氨酸含量可知,低温的胁迫影响了植物叶片游离脯氨酸的含量变化,植物的抗寒性与游离脯氨酸有关;低温胁迫下三种植物叶绿素含量随着温度的下降,叶绿素含量、叶绿素a、叶绿素b和叶绿素a/b均呈下降趋势,狗牙根对低温胁迫最为敏感;(3)低温锻炼前后,丛毛羊胡子草和金发草的土壤过氧化物酶与脲酶活性的绝对值均大于狗牙根的,特别是过氧化物酶,说明丛毛羊胡子草和金发草的酶活性大于狗牙根的。

The rule of growth and decline about main biochemical material (solubility protein, solubility sugar, SOD and gossypol IAA) contacting with stomatic embryogenesis showed that content of solubility protein and SOD activity was differed in the different period, being lower in earlier callus and reaching maximum in 120 day.

对陆地棉愈伤组织诱导及体细胞胚发生过程中的主要生化物质(可溶性蛋白含量、可溶性糖含量、SOD活性和IAA氧化酶活性)的测定表明,各个时期的可溶性蛋白含量、SOD活性是不同的,在愈伤组织形成初期较低,随着愈伤组织的生长及胚状体的形成,可溶性蛋白含量、SOD活性逐渐上升,在120d时达最大值;而可溶性糖含量在60d时有一个高峰值,在120d时达到最低值;IAA氧化酶活性在60d、120d时酶活较高,90d的酶活较低。

The composition and structure of Soybean Protein Isolate are first introduced,Then the functional modification of SPI via chemical ways which include the reaction of amino group,carboxyl group and sulphydryl group,via enzymic ways which include the modification using transglutaminase enzyme and papain,via physical ways which include blending and heat treatment are reviewed.

首先介绍了大豆分离蛋白的基本组成与结构,然后分别从化学改性、酶改性和物理改性三个方面对大豆分离蛋白改性进行了综述。其中,在化学改性方面,针对大豆分离蛋白中含有的氨基、羧基、巯基等不同活性基团的改性原理及研究现状进行了介绍。在酶改性方面,主要介绍了谷胺酰胺转胺酶、木瓜蛋白酶等对大豆分离蛋白的改性作用。在物理改性方面,介绍了共混、加热改性等目前研究较多的方法。

Human hair keratin ; spinal cord injury ; rat ; tissue engineering ; tissue-engineered spinal cord ; connector ; morphology ; praxiology ; neuron ; axis-cylinder ; nerve fiber ; myelin sheath ; astrocyte ; oligodendrocyte ; microglia ; ependymal cell ; neural stem cells ; macrophage ; multinuclear giant cell ; lysosomal enzyme ; ubiquitin system ; suppression subtractive hybridization ; gene express ; CytohromeoxidaseⅢ; amino acid transporter system A2(Ata2); Peroxiredoxins 5(prd 5); cysteine proteinase inhibitor cystatin c

国家自然科学基金;广东省自然科学基金人发角蛋白;脊髓损伤;大鼠;组织工程学;组织工程化脊髓;桥接物;形态学;行为学;神经元;轴突;神经纤维;髓鞘;星形胶质细胞;少突胶质细胞;小胶质细胞;室管膜细胞;神经干细胞;巨噬细胞;多核巨细胞;溶酶体酶;泛素肽酶系;抑制消减杂交;基因表达;细胞色素氧化酶Ⅲ;氨基酸转运蛋白系统A2;抗过氧化物蛋白5;半胱氨酸蛋白酶抑制剂

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明,除了能复性成天然态的溶菌酶分子外,还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体;而它们的还原电泳和在SDS存在时的复性结果表明,这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成,而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

The ammo acid sequences deduced from the other ESTs are homologous to glutamine synthetase, lipase-like protein, decarboxylase family protein, putative AUXl-like permease, HT-ATP synthetase, Proline-rich protein, RNA transporter binding protein and cation-transporting ATPase, respectively.

摘要其它ESTs的编码产物分别与谷氛酞胺合成酶、类似脂酶、脱数酶家族蛋白、H+- ATP合成酶、激素类渗透酶、阳离子运输ATPa se、RNA运输因子结合蛋白和富含脯氮酸的蛋白等同源。

This experiment was made to investigate the changes of lipopro tein li pase and hepatic lipase activity and their effects on gallstone formati on in rabbit model in which the stones were induced by high cholesterol diet.Act ivities of plasma LPL and HL were determined; other data including concentratio n of plasma lipoprotein cholesterol, concentration of bile cholesterol and bile acids were also obtained.

为研究肝脂酶及脂蛋白脂酶在胆固醇结石成石过程中的变化及其对成石的影响,采用高胆固醇膳食诱发兔胆囊胆固醇结石模型,观测对照组及高胆固醇膳食1、2 、3、4周组动物血浆脂蛋白脂酶、肝脂酶活性、血浆脂蛋白胆固醇及胆汁中甘氨胆酸、甘氨脱氧胆酸、胆固醇的变化。

The separation purities by new method were determined and compared with those of some traditional methods by SDS-PAGE gradient electrophoresis.

其次,采用中性蛋白酶、碱性蛋白酶等几种常用的蛋白酶对分离的7S和11S球蛋白进行酶解比较实验,发现在相同条件下,7S比11S酶解后的酸溶多肽得率高,7S比11S相对更容易被酶解;通过扫描电镜和SDS-PAGE电泳分析7S和11S的酶解产物,表明7S和11S分子酶解前后的变化明显,但两种大豆蛋白经酶解作用后,均有相当部分未被酶解的蛋白分子残留在酶解残渣中,而酶解离心后的上清液中则已基本不存在原来的蛋白质大分子。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

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Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。