酶蛋白

However, the oligonucleotide is easy to be discomposed by nuclease and difficult to reach the target.

肽核酸既不是典型的核酸也不是典型的蛋白，它能抵抗核酸酶和蛋白酶的分解。

In order to research the effects of minimum tillage and no-tillage patterns on yield and senescence of flag leaf of winter wheat, four tillage patterns were adopted in Longkou city, Shandong Province, China for three years. Four tillage patterns were as follows: conventional tillage with straw turnover, rotary tillage with straw turnover, serrated disk harrow tillage with straw turnover, no-tillage with straw cover. Effects of different tillage patterns on the activities of superoxide dismutase, peroxidase and the contents of malondialdehyde and soluble protein of the winter wheat flag leaves after anthesis were analyzed.

为了研究少免耕耕作体系对冬小麦旗叶衰老状况的影响，采用4种土壤耕作模式（常规耕作秸秆还田、旋耕秸秆还田、耙耕秸秆还田、免耕秸秆覆盖）在山东龙口进行了3年田间试验，研究了耕作模式对小麦产量及花后不同时期旗叶超氧化物歧化酶和过氧化物酶活性、丙二醛和可溶性蛋白含量的影响。

Since the dismutation of superoxide radicals by SOD is a reaction that is rate-limited by diffusion and enhanced by electrostatic guidance, all these structural features may be related to GP-MnSOD's high catalytic activity: first, Lys38 may increase the effective enzyme-substrate association rate by enhancing the electrostatic gradient at its substrate channel; second, an excess of acidic over basic residues makes the negative surface potential of GP-MnSOD more larger than those of other known MnSODs. This may enhance the reaction rate by reducing futile collisions of superoxide radicals with GP-MnSOD's surface.

因此，GP-MnSOD的上述结构特征可能与GP-MnSOD的较高酶活有关：首先，Lys38增加了底物通道口的正静电势梯度，从而可能促进了GP-MnSOD的活性中心与底物的有效结合速率；其次，GP-MnSOD表面覆盖的过量酸性残基使得它比其它MnSOD在底物通道以外的蛋白表面更多地被负静电势所覆盖，这可能有效地减少了超氧阴离子与GP-MnSOD表面的无效碰撞，从而提高了反应速率。

The results showed that the inorganic and organic fertilizers can increase soluble sugar and soluble protein content of leaves, and it can enhance the activities of NR, and whats more, it may adjust assimilation and output of nitrogen and stave doat, provide the valid energy for accumulation of kernel nutrition substances, at the same time, it make high-oil maize mature lively.

有机无机肥料配合施用可增加叶片可溶性糖和可溶性蛋白含量，提高叶片硝酸还原酶活性，从而增强叶片细胞渗透调节能力，调节植株对氮素同化和输出，延缓衰老，为籽粒营养物质的积累提供了有效能量，也保证了高油玉米活杆成熟青贮加工的要求。

The signal peptides of chicken Igλ light chain and murine plasmingen were fused to the GFP N terminus using site-directed mutagenesis to construct the vectors with signal sequence, pEGFP-SPc and pEGFP-SPm. The chicken Igλ gene was inserted into the two vectors with signal sequence after double enzyme cutting.

分别将鸡Igλ轻链信号肽、小鼠纤溶酶原信号肽与pEGFP-C1载体的绿色荧光蛋白N端融合，产生带有信号肽的中间载体pEGFP-SPc和pEGFP-SPm。

At the same time, some problems were found during separations of 7S and 11S globulin and hydrolysis of soybean. According to the structure of soybean protein, this paper studied the separation methods of 7S and 11S globulin, and a new separating method was put forward: with low-temperature defatted soybean draff as raw material, a technique of alkaline extraction, acidic precipitation and membrane filtering was used.

为了进一步探讨7S和11S两种功能性不同的大豆球蛋白的分离新工艺和方法，以及更好的解决大豆蛋白难酶解的问题，本文从大豆蛋白质原料的结构入手，选择低温脱脂大豆粕为原料，首先研究了7S和11S大豆球蛋白的分离方法，通过几种传统分离方法的比较实验，提出了一种碱提酸沉结合膜分离技术的分离新工艺，SDS—PAGE电泳分析表明碱提酸沉膜分离技术得到的7S和11S大豆球蛋白的相对纯度分别达到75.5％和84.7％，结果均优于传统方法。

Key words: Echinococcus granulosus;thioredoxin peroxidase;gene cloning;sequence analysis

细粒棘球绦虫；硫氧还蛋白过氧化物酶；基因克隆；序列分析

In this work, crystal conditions of lysozyme and albumin egg were studied using membrane crystallization technique.

本文用膜结晶技术对溶菌酶和卵清蛋白的结晶条件进行了实验研究。

By means of the progressive papain enzyme antipyretic degeneration egg albumin filtered the appropriate conditions.

逐一递进的方式分别对木瓜蛋白酶酶解热变性鸡蛋清蛋白的适宜条件进行筛选。这句话翻译成英文

Enzymic hydrolyzates of gluten showed good water-solubility and emulsibility in the wide range of pH, including near its PI.

水解度控制在8%～15%，可使酶解面筋蛋白在广泛pH范围，特别是等电点附近，均具有优良的水溶性和乳化性能。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。