酶蛋白

The optimal conditions of the pepsin hydrolysis of frame protein in catfish Claris lareza were investigated using a general-purpose rotation unitized design of the second power of four factors, and the content of soluble peptides was determined in the hydrolytic products. The Optimization of Design Expert 7.1.2 revealed that antibacterial activity of the frame protein hydrolysates was controlled by regulation of four process conditions.

采用四因子二次通用旋转设计，以抑菌率为测定指标，对胃蛋白酶水解革胡子鲶Claris lareza鱼骨蛋白的条件进行了优化，同时测定了酶解产物中可溶性肽的含量，以分析抑菌率与可溶性肽含量之间的相关关系。

The new method can also be used to predict the cleavability of an oligopeptide by HTV-2 protease and it also yielded a very high rate of correct prediction for both the training and testing sets.

这种新方法也可以用来预测HTV-2蛋白水解酶寡肽的断裂，试测和实测中也得到了很高的预测率。

The mean accuracy (Kabsch and Sander, 1983) in predicting the cleavability by HTV-1 protease for the 94 oligopeptides with the 2-4—6 subsite-coupled model developed in this article is 97.9%, while that by the h function method for the same set of data is 85.1%.

在这篇文章中创建的2-4—6亚位点耦合模型预测94个寡肽的HTV-1蛋白水解酶断裂的平均精度(Kabsch and Sander， 1983)是97.9%，而用h功能法得到的预测数据是85.1%。

The coagulase in filtrate of Staphylococcus aureus was a complex of sugar and protein, with antigenic activity, and might play a role in the function of filtrate of Staphylococcus aureus.

金黄色葡萄球菌滤液中凝固酶为一类糖与蛋白复合物，具有抗原活性，可能在金黄色葡萄球菌滤液的功效中发挥作用。

During liquefaction of the ejaculate, the semen coagulum proteins semenogelin I (SEMG1) and semenogelin II (SEMG2) are degraded to low molecular mass fragments by kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen.

射精后精液液化的过程中，精液凝结蛋白semenogelin I（SEMG1）和semenogelin II（SEMG2）被血管舒缓素相关肽酶3（kallikrein-related peptidase 3，KLK3）降解成小分子量片断。KLK3也被称作前列腺特异抗原。

Protoscoleces of cyst were recovered from the brain of sheep naturally infected with coenurus of Taenia multiceps and total RNA was extracted.T.multiceps thioredoxin peroxidase cDNA fragment was amplified by RT-PCR from the total RNA,ligated into pMD18-T vector and sequenced.

从自然感染的病羊脑内采集多头蚴原头节，提取总RNA，采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶基因片段，PCR产物连接到pMD18-T载体，转化至大肠杆菌DH5α后测序，发现克隆到的TmTPx基因片段开放阅读框为453bp，编码150个氨基酸。

The bioinformatics analysis showed that the gene had the highest homology with the gene of Penicillium janthinellum, which reached up to 76%. The first 26 amino acids might be a signal peptide sequence with the hydrophobicity of up to 2.63. The CBHⅠ gene consisted of the catalytic domain, convergence zone and cellulose-binding domain and its tertiary structure was mainly composed of 3-sheets.

分别扩增到鹅源草酸青霉F67纤维素酶CBHⅠ基因片段A(EU574736)、5'端序列B1(EU603295)、3'端序列B2(EU652768)及全长基因C(EU727171)，并成功构建真核表达载体pPIC9K-CBHⅠ；生物信息学分析表明，该基因蛋白序列与微紫青霉氨基酸序列同源性最高，达76%，前26个氨基酸为信号肽序列，疏水性可达2.63，由催化功能域、衔接区和真菌性纤维素结合域构成，其三级结构主要为β-sheet。

In the study, protein components in cottonseed meal and enzymatic hydrolysates of CSM were analyzed by general methods, gel filtration chromatography and high performance liquid chromatography.

通过常规测定、凝胶层析、高效液相色谱等3种方法，测定和分析了棉粕蛋白及其枯草蛋白酶酶解产物的组份，并进行比较研究。

Under the conditions, the degree of hydrolysis and solubility of zymolytic cottonseed protein were 10.87% and 63.48%, respectively.

在此条件下，酶解棉籽蛋白的水解度为10.87%，溶解度为63.48%。

Methods: Virus proliferation assay, cell viability assay and Western blot were performed to assess the selective replication and cytolysis of CNHK500 in telomerase positive liver cancer cells Hep3B, HepGⅡ, SMMC7721 and in normal cells.

方法利用病毒增殖实验、细胞活力实验、蛋白印迹分析来检测增殖病毒CNHK500在端粒酶阳性的肝癌细胞株HepGⅡ、Hep3B、SMMC7721及正常细胞中选择性增殖和溶解细胞的特性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。