酶蛋白

After synthesis of the DNA polymerase and replication of DNA,the viral capsid and glycoproteins are synthesized.

在DNA多聚酶合成DNA复制后，病毒衣壳和糖蛋白合成。

Two virus RNA strands and replication enzymes then come together and core proteins assembly around them forming the capsid.

两条病毒RNA及复制酶接著和周围的核心蛋白组合成衣壳。

MIXED MICROBIAL CARBOHYDRASE and PROTEASE from BACILLUS SUBTILIS, Var.

混合微生物糖酶和蛋白酶类(源自改良的枯草芽孢杆菌)。pdf

The carboxyhemoglobin levels of in-flowing pulmonary blood and out-going pulmonary blood were determined at 2,4 and 6 hours after treatments. Malondialdehyde contents and superoxide dismutase activities in the lung,liver and blood were also determined. Pathological changes in lung and liver were examined with light microscope,and immunohistochemical technique was used for analysis of heme oxygenase-1 (HO-1) protein expression and distribution in lung and liver.

各组分别在制模后2、4和6 h测定出入肺血中碳氧血红蛋白水平；肺、肝组织及血液中丙二醛含量及超氧化物歧化酶活性；光镜下观察肺、肝组织形态学改变；免疫组化分析血红素加氧酶-1(HO-1)在肺、肝组织中的蛋白表达和分布。

Naturally occurring manganese proteins like pyruvate carboxylase[4],avimanganin[5] and concanvalin A[6]have been isolated.

自然产生的丙酮酸羧化酶等[4]，avimanganin [5锰蛋白]和刀豆甲[6]已经isolated。

Four differentially expressed cDNA had been cloned and made function analysis. Homology search in Genbank showed that there were two new sequences; the other is methyl-binding domain protein , phosphoenolpyruvate carboxylase , respectively.

同时，对4个Northern杂交验证的差异表达片段进行了克隆测序，结果表明，有2个为新基因，其余的2个片段可能分别为甲基化区结合蛋白和磷酸烯醇式丙酮酸羧化酶。

The binding domain of catalase with EV71 2A protein was also identified using the same system.

过氧化氢酶与肠病毒71型2A非结构蛋白的结合区域也经由相同的系统定位出来了。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The results showed that the technique was useful for the separation of simulated protein mixture and simple cellulase compound.

结果显示，置换层析技术对模拟混合蛋白和简单纤维素酶混合物具有很好的分离作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。