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The method uses bacteriolysis enzymatic k of enzymatic broken wall, albumen and sds divide albumen, extract mangrove actinomyces dna, earning dna can undertake 16srdnapcr enlarge is added directly detect, have identification to actinomyces.

方法利用溶菌酶破壁、蛋白酶k和sds除蛋白,提取红树林放线菌dna,所得dna可直接进行16srdnapcr扩增检测,对放线菌进行鉴定。

Symptoms, physical signs, serum concentration of BUN, Cr, AST, ALT, AMS were observed and compared between two groups. The changes of C-reactive protein and result of bacteria culture, APACHEⅡ grades and Balthazar CT grades, open-belly surgery rate, complications, mortality rate, average hospital stay and costs were compared between these two groups.

比较2组患者的生命体征、临床表现、血糖、氧合指数、肝肾功能、淀粉酶、脂肪酶、C-反应蛋白的变化、细菌培养阳性率、治疗前及治疗后的Balthazar CT评分、APACHE Ⅱ评分、开腹手术率、并发症发生率、死亡率、治愈率、平均住院时间以及费用。

A series of enzymes involved in the process of tumor cells and endothelial cells's migration across basement membrane and extracellular matrix.

肿瘤细胞和内皮细胞穿过基底膜和细胞外基质的迁移过程需要一系列蛋白水解酶的参与。

Biliverdin was biliverdin reductase reduced to bilirubin, and free iron and transferrin binding.

胆绿素随后被胆绿素还原酶还原成胆红素,而游离铁与转铁蛋白结合。

We analyse the adjustment of the birch reeds to the cold and drought from the conductance, K+ filtrate, SOD, POD, MDA, content of the water-soluble protein, penetration of the bioplasm film, photosynthesis and fluorescence.

3通过对欧洲优良无性系在寒冷、干旱的胁迫下电导率、钾离子的渗透率、酶促防御系统,膜质过氧化物,可溶性蛋白含量、原生膜透性和光合、荧光等生理指标的变化,对欧洲白桦优良无性系苗期的适应性进行分析。

The cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology.In the experiment, the full code sequence of bar gene was cloned by PCR from transgenic herbicide resistant Bobwhite wheat and checked. It was expressed in E.coli and its protein was determined.

本实验从抗除草剂转基因Bobwhite小麦中,利用PCR克隆的方法扩增出bar基因全长,并在原核表达系统中表达,鉴定表达蛋白的活性,将能够正确编码PPT乙酰转移酶的bar基因片段,经过适当的修饰构建入真核表达载体。

Methods Traditional bromelin technology, the anti-globulin test and three-stage polybrene test were used to determine the titre of Rh, MNSs, Duffy and Kidd blood system irregular antibody to measure their sensitivity and specificity.

采用蛋白水解酶法、抗球蛋白法及聚凝胺三步法试验平行检测一批Rh、MNSs、Duffy、Kidd等系统的不规则抗体效价,观察TSPT等的敏感性。

Methods The experimental TBI model was established by bumpiness of free falling body according to Feeney′s.The rats′cerebral edema were imaged with magnetic resonance image,The changes of brain water content and permeability of blood-brain barrier were measured by the methods of wet and dry weight and Evans blue fluorometry.The expression of AQP-4 mRNA was examined by reverse transcriptase polymerase chain reaction.

按照改进的Feeney自由落体撞击法建立大鼠创伤性脑损伤模型,用磁共振成像对大鼠脑水肿进行检测,干/湿比重法和伊文思蓝测定法观察大鼠TBI后不同时相脑组织含水量和BBB通透性的变化,并采用逆转录聚合酶链反应法检测AQP-4 mRNA的表达,蛋白质免疫印迹法检测AQP-4蛋白的表达。

Sclerostin expression and distances of each osteocyte to the canal surface and cement line were assessed for all osteonal osteocytes in 636 unremodelled osteons chosen from fields ( approximately 0.5mm diameter) with at least one canal staining for alkaline phosphatase (ALP; a marker of bone formation).

我们评估了对636个未重塑的骨单位中所有的骨单位骨细胞的硬化蛋白表达和每个骨细胞到管道表面和粘合线的距离,这些骨单位从至少一个管道碱性磷酸酶(ALP;骨形成标志物)染色的区域选择(直径大约0.5mm)。

Results Nine days after ulcer induction, the ulcer area was 11.9±3.1mm^2 and 19.7±3.8mm^2 in rats with normal saline and celecoxib treatments, respectively (P.01). The total acidity of gastric juice and the expressions of H(superscript +), K(superscript +)-ATPase mRNA and protein in celecoxib group were significantly higher than that in normal saline group at both 6 and 9 days after ulcer induction, but no significant difference was found between the two groups in the amount of secretary canaliculus and microvillus.

结果 制模术后第9日,生理盐水组和塞来昔布组的溃疡面积分别为11.9±3.1和19.7±3.8(P.01);制模术后第6日和第9日,塞来昔布组胃液总酸度和H,K-ATP酶mRNA和蛋白表达水平均显著高于生理盐水组,而两组壁细胞的分泌小管和微绒毛数量则均无明显差异。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。