酶蛋白

A novel method for the synthesis of molecular probe of ubiquinone binding protein is described.

以 3 硝基水杨酸和正癸烷为原料，合成了研究线粒体呼吸链酶系中各泛醌结合蛋白的分子探针，即氚标记的 3 叠氮基 N 正癸烷基水杨酰胺，为进一步研究泛醌结合蛋白提供了有效工具。

Methods Expression of C-erbB2、nm23 and p53 proteins was retros pectively studied by immunohistochemistry, and Kaplan-Meier Method and Cox's proportional hazard regression model were used to analyze the relationship of their expressions with prognosis.

方法应用链霉菌抗生物素蛋白-过氧化酶连接法检测70例卵巢上皮性癌石蜡切片中C-erbB2、nm23和p53蛋白的表达情况。

Methods Expression of C-erbB2、nm23 and p53 proteins was retros pectively studied by immunohistochemistry, and Kaplan-Meier Method and Coxs proportional hazard regression model were used to analyze the relationship of their expressions with prognosis.

方法应用链霉菌抗生物素蛋白-过氧化酶连接法检测70例卵巢上皮性癌石蜡切片中C-erbB2、 nm23和p53蛋白的表达情况。

Perceived by a small family of F-box proteins including transport inhibitor response 1 (TIR1), auxin regulates gene expression by promoting SCF ubiquitin-ligase-catalysed degradation of the Aux/IAA transcription repressors, but how the TIR1 F-box protein senses and becomes activated by auxin remains unclear.

植物生长素通过促进SCF复合体泛素连接酶催化的Aux/IAA转录抑制蛋白降解，可影响到包括转运抑制效应因子 TIR1在内的一个F-box蛋白小家族，但是对植物生长素如何使TIR1感应并激活仍未了解。

The 329 bp terminal inverted repeat sequence can't form the conserved fold-back secondary structure as that of many Streptomyces linear replicons. Lacking of typical Streptomyces tap/tpg locus for te-lomere replication, pPR2.3c encodes a protein with two domains resembling the telomere associated protein of Strepto-myces and helicase of Haemophilus respectively. No typical Streptomyces iteron-rep locus for replication from the cen-trally located origin, two DNA fragments containing almost all pPR2 were cloned and introduced by transformation into S.

其端粒末端反向重复序列的长度为329 bp，不能像多数链霉菌的线型质粒那样能形成保守的&折返&的二级结构。pPR2虽然没有参与链霉菌端粒复制的保守的tap/tpg基因，但是pPR2.3c基因编码了一个双结构域蛋白，分别同链霉菌的端粒复制相关蛋白Tap和嗜血杆菌的解旋酶具有相似性。pPR2缺少典型的链霉菌重复序列-复制基因区段，将几乎覆盖全长pPR2的两段DNA进行克隆后，不能转化变铅青链霉菌。

This correlated with reduced apoptosis in cardiomyocytes of Gq mice. The anti oxidatie properties of E2 were also associated with increased expression of thioredoxin, Trx reductases, and Trx reductase actiity in the hearts of Gq mice.

这和减Gq老鼠心肌细胞凋亡相关。E2的抗氧化特性也和硫氧还蛋白、硫氧还蛋白还原酶的表达及其活性的增加有关。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

When coupled with overexpression of Delta, deregulation of the expression of Pipsqueak and Lola induces the formation of metastatic tumours.

这个过程需要有两种组蛋白修饰酶和染色质域蛋白Polycomb的存在。

Glutamine-binding protein is one of the ligand-specific periplasmic binding proteins in the Escherichia coli permease systems, predicted to play an important role in transferring Gln from the pe-riplasmic space to the cytoplasmic space.

谷氨酰胺结合蛋白(Glutamine-binding protein， GlnBp)是大肠杆菌透性酶系统中一个细胞外液底物专一性结合蛋白，对于细胞外液中谷氨酰胺的运输和传递至关重要。

There are some questions in our country if using this approach.The application of protein hydrolysate to infant formula milk powders...

本文介绍了我国工业化酶法水解牛乳蛋白还存在的问题及水解蛋白在婴幼儿配方奶粉中的应用阻力。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。