酶蛋白

Recently, it is found that the neuron apoptosis caused by brain ischemia is associated with signal pathway of endoplasmic reticulum stress, which is different from the traditional apoptotic pathway. Caspase-12 has been identified as a molecule which initiates apoptosis selectively in response to ER stress and is not activated by other pathways. Caspase-12 is localized on the ER membrane, but once specifically activated by ER stress, it translocates to the cytoplasm.

最近的研究发现，这种细胞凋亡与内质网应激(endoplasmic reticulum stress ER stress)有关，其是一种不同于经典的凋亡途径的新途径，分子伴侣葡萄糖调节蛋白78(glucose regulated protein， GRP78/Bip)表达增高是反应ER stress的最敏感的指标，胱冬酶-12(caspase-12)为鼠源性的内质网膜上的蛋白，它的特点是只被内质网诱导的凋亡所激活而不被其它非内质网因素所激活。

Results Serum albumin and prostatic acid phosphatase were reduced in seminal plasma from idiopathic male infertility,while Cathepsin B and Zn-alpha-2-glycoprotein were increased.

结果特发性男性不育患者较正常对照组精浆中血清白蛋白和前列腺酸性磷酸酶减少，而组织蛋白酶B和锌α2糖蛋白增加。

The effect of the concentration, pH of several ligands such as acetate, oxalate, tartrate and phosphate solution and their orders added on the adsorption and activity of BSA and acid phosphatase on yellow brown soil, latosol, kaolinite, goethite and -MnO2 was first systematically studied by using batch and FTIR methods after analyzed and studied the relative literature that reported in the world.

论文在分析总结了国内外有关研究的基础上，用批量法和傅立叶变换红外光谱法首次系统地研究了乙酸、草酸、酒石酸和磷酸几种配体的浓度、pH及加入顺序对牛血清白蛋白、酸性磷酸酶在黄棕壤、砖红壤、高岭石、针铁矿和二氧化锰表面的吸附行为及活性的影响，探讨了这些配体对蛋白吸附机理及二级结构的影响。

The effect of the concentration, pH of several ligands such as acetate, oxalate, tartrate and phosphate solution and their orders added on the adsorption and activity of BSA and acid phosphatase on yellow brown soil, latosol, kaolinite, goethite and δ-MnO〓 was first systematically studied by using batch and FTIR methods after analyzed and studied the relative literature that reported in the world. The impact of these ligands on the adsorption mechanism and secondary structure of protein was simultaneously engaged.

论文在分析总结了国内外有关研究的基础上，用批量法和傅立叶变换红外光谱法首次系统地研究了乙酸、草酸、酒石酸和磷酸几种配体的浓度、pH及加入顺序对牛血清白蛋白、酸性磷酸酶在黄棕壤、砖红壤、高岭石、针铁矿和二氧化锰表面的吸附行为及活性的影响，探讨了这些配体对蛋白吸附机理及二级结构的影响。

SPI was hydrolyzed by double enzymes in order to reduce polypeptides bitter-tasting in this experimentation.

试验采用双酶水解大豆分离蛋白的方法去除肽基大豆蛋白水解液的苦味。

Results: CAP did not affect cell proliferation of HDFC, but increased the ALP activity of HDFC at high protein concentration.

结果：牙骨质附着蛋白对牙囊细胞的增殖无显著影响，但高浓度的牙骨质附着蛋白可增强牙囊细胞碱性磷酸酶活性。

According to the results, we conclude that recombinant vector could express bioactive IL-12 from transfected murine fibroblast cells.Key words:interleukin-12; fusion protein; transfection; polymerase chain reaction; glycine linker

为此，本文以多聚酶链反应技术，将2个编码基因通过接头序列连接在一起，构建了融合蛋白的表达载体，并证明这种表达的融合蛋白具有天然IL-12的生物学活性。

Preliminary studies on affinity isolation based on the specific combination between Biotin and Avidin Presently, affinity chromatography is increasing to be a main protein isolation and purification technique for a complicated system because of its high specificity, high rate of recovery, etc.

利用生物素和抗生物素蛋白间的特异性结合作用，将生物素通过化学反应键合到蛋白质的某种官能团上，酶解后的肽段通过固定了抗生物素白蛋白的亲和分离凝胶色谱柱，可以选择性地分离生物素化的肽段，达到简化复杂体系的目的。

Second is studying the traumatic condition changes in a choosey pressure at different post-PT phases. Moreover, we detected model PLA_2 activity, express of PLA_2 subtype mRNA by fluorescent quantitative RT-PCR technique, protein express and site by Western blot and IHC at different post-PT phases. At last, we studied the PLA_2 inhibited effects and model protection of Variabilin in vivo.

研究在不同压强的外力作用下，模型指定时相点的一般情况和存活率、大体、光镜及电镜下胰腺组织的病理变化和常规酶学变化，得到稳定建模致伤压强；然后，固定致伤压强，研究胰腺创伤后不同时相点的伤情变化(包括一般情况、胰腺组织的病理变化、AMS和LPS活性变化、血常规、血生化、胰腺细胞凋亡以及胰腺组织细胞生长周期变化情况)；再次，采用3H-花生四烯酸标记大肠杆菌膜作为底物的方法检测大鼠胰腺创伤后不同时相点的PLA_2活性，利用荧光定量RT-PCR技术检测其亚型mRNA的表达，利用Western blot和IHC技术检测其蛋白表达量和蛋白组织定位；最后，将Variabilin体内应用于急性胰腺创伤大鼠模型，研究其对模型的保护作用和对PLA_2的抑制效应。

Coli culture . The recombinant enzyme was purified, dialysed and renatured, and exhibited arginine esterase activity, thrombin activity and fibrinogen cleaving activity without being released from the fusion partner. In animal model the recombinant protein significantly reduced rat blood fibrinogen level and inhibited experimental thrombus formation in rabbit.

融合蛋白通过纯化、透析复性，在未经肠激酶切的情况下，即测得精氨酸酯酶活性、凝血酶活性及纤维蛋白原降解活性；动物实验表明重组蛋白能降低大鼠体内纤维蛋白原的含量和抑制家兔实验性血栓形成。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。