酶蛋白

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

The profiles of some silk proteins and isoenzymes were examined by SDS-PAGE. The results showed that there were no revealable changes in the structure proteins and other high expressive protein examined.

5用SDS-PAGE检测了几种丝蛋白质及其同工酶的表型，结果表明高压静电场处理没有引起检查的结构蛋白和其他高表达蛋白发生可检测到的变异。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Desmin, the marker of myoblasts, was adopted to identify the expression of specific marker protein of sarcoblast desmin with immunohistochemistry. Desmin negative cell clones were removed, and desmin cell clones were cultured continuously with the culture fluid replaced once every other day.

采用成肌细胞特异性标志抗原desmin免疫化学染色，鉴定成肌细胞标志蛋白——结蛋白的表达，弃去desmin阴性的细胞克隆，继续培养desmin阳性的细胞克隆，隔天换液1次，7 d进行酶消化传代，获得大量扩增的细胞，并可冻存复苏，用于实验。

Methods Seral anti-P 53 antibody was detected with immuno-PCR.The expression of P 53 in tissue was detected with enzyme imunohisto-chemistry technique.

采用免疫PCR方法检测血清抗P 53 蛋白抗体，酶免疫组化方法检测组织P 53 蛋白表达。

We examined the effects of temperature and photoperiod on the thermogenic characteristics of brown adipose tissue in plateau pikas.

测定了高原鼠兔在驯化2周后(分4种处理：23℃，16L：8D；23℃，8L：16D；5℃，16L：8D和5℃，8L：16D)褐色脂肪组织的蛋白含量、线粒体蛋白含量和细胞色素c氧化酶活性的变化。

On the basis of purification and immunity of vitellin, antibodies of vitellin were obtained and used to measure the concentration of vitellin in embryo in different stages of embryonic development by enzyme-linked immunosorbent assay.

在采用纯化的卵黄磷蛋白免疫兔子而获得其抗体的基础上，运用酶联免疫吸附检测法(enZyme一linked ilnlnunosorbent assay，ELISA)分析测定了发育过程中胚胎内卵黄磷蛋白的含量。

The zymolytic cottonseed protein had higher solubility in pH 1-11, and it still maintained the acid soluble character.

溶解度分析结果表明，限制性酶解棉籽蛋白在pH 1~11范围内均具有较好的溶解度，仍然保持了棉籽蛋白酸溶性的特点。

Results TNFα-induced ICAM-1 expression in HK-2 cells was increased in both protein and mNRA levels( P .01). NFkB inhibitor N-tosyl phenylalanine chlormethyl ketonecould inhibit these effects of TNFα.

方法用 HK-2 细胞作靶细胞，用细胞酶联免疫吸附法和 Northem 杂交观察 ICAM-1 的蛋白和基因的表达，以电泳迁移率变动法测定转录因子核因子 kB和激活蛋白 1(AP-1)的活性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。