酶蛋白

Methods After L-02 cells were incubated with 40, 80, and 160μg/ml sodium fluoride for 24 hours, the cell survival rate, the level of P53 protein and mRNA expression was assessed by MTT, Western blot and RT-PCR analysis respectively. The cell survival rate in L-02 cells treated by high concentration of fluoride was significantly lower than that in the control group.

方法］对体外培养的人胚肝细胞染毒氟化钠40、80、160μg/ml 24h后，分别采用MTT试验、蛋白印迹技术和逆转录－聚合酶链式反应方法检测人胚肝细胞存活率、P53蛋白和mRNA水平。

An indirect competitive enzyme-linked immunosorbent assay was established for detecting vitellogenin in the whole body homogenate of male swordtail fish.This technique was developed with antiserum of lipovitellinresistance as antibody and Vtg as antigen.

以卵黄脂磷蛋白(lipovitellin，Lv)抗血清为抗体，以纯化的卵黄蛋白原(vitellogenin，Vtg)为抗原，建立了间接酶联免疫吸附反应方法检测剑尾鱼雄鱼整体匀浆液中ρ。

Protein phosphorylation and dephosphorylation and zymogen activation are two very important regulation mechanism for enzyme activity and function.

本文第三章对蛋白激酶催化磷酸化酶活化的三种可能机制—随机激活模型和ATP与激酶先结合的顺序结合激活模型及ATP与蛋白底物先结合，再与激酶结合的模型给予解析。

Results of Western-blot reveal: along with the increase concentration of taxol, the expressions of Fas、FasL proteins increased gradually, while the expressions of Caspase-8、Caspase-3 zymogen proteins decreased gradually.

Western-blot结果显示：随着紫杉醇浓度的增加，Fas、FasL蛋白表达量逐渐增加，Caspase-8、Caspase-3酶原蛋白表达量逐渐减少。

The results were as follows:(1) The method that HA was treated by ethanol of 2.5x volume for 20 hours turned out to be the most economic and efficient.(2) Zymolysis combined with deproteinization by chloroform was better than deproteinization by chloroform for several times in yield and purity of HA.(3) HA with purity of 71.1 60/o was obtained by the fermentation with cetylpyridinium chloride.

本文对发酵液中HA的分离提取方法进行对比研究，结果表明采用2.5倍体积的乙醇对HA沉淀，作用时间20小时最为经济合理；采用酶解结合氯仿除蛋白较之氯仿除蛋白，HA的产量和纯度均有所提高；经氯代十六烷基吡啶分级分离后，可得纯度为71.16％的HA产品。

Protein in fruiting body of Armillaria luteo-virens Sacc is high. It is effective way to hydrolyse protein of fruiting body in Armillaria luieo-virens Sacc by enzymes to produce active peptides with biological function.

黄绿蜜环菌(Armillaria luteo-virens Sace)子实体中蛋白含量很高，采用酶水解黄绿蜜环菌子实体蛋白的方法是生产具有生物学功能特性的活性肤的有效途径。

On the cell surface, CathA, Neu1, and the enzymatically inactive splice variant of GAL form the elastin-binding protein complex.

在细胞表面，CathA，、Neu1和GAL的酶灭活的剪切变体共同组成弹力蛋白结合的蛋白复合物。

Firstly, MiCy, Citrine, mKO were inser into expression vectors of pRSETB, and then MiCy, Citrine,mKO were expressed in E.coli and purified by using Ni-NTA collum chromatography.

首先，构建了含有六个组氨酸标签的三种荧光蛋白MiCy、Citrine和mKO，而后在大肠杆菌中表达了MiCy、mKO和Citrine三种蛋白，并用金属鳌合亲和层析进一步纯化，纯化产物使用特异性酶去除His-tag。

The efficacy of this technique for protein mapping was demonstrated by the mass spectral analyses of the peptide fragmentation of several proteins, including lysozyme, albumin, conalbumin, and ribonuclease A.

我们利用四种蛋白质，溶菌蛋白、白蛋白、伴蛋白和核醣核酸酶A的胜肽片段之质谱分析而证明这个方法是非常有效率的。

A novel class of autoantigens of anti-neutrophil cytoplasmic antibodies in necrotizing and crescentic glomerulonephritis: the lysosomal membrane glycoprotein h-lamp-2 in neutrophil granulocytes and a related membrane protein in glomerular endothelial cells.

PMID 7836914一种新型的类自身的抗中性粒细胞胞浆抗体和细胞坏死性新月体肾炎：在溶酶体膜糖蛋白氢灯- 2的中性粒细胞和相关的膜蛋白在肾小球内皮细胞。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。