酶蛋白

Hexon protein gene of egg drop syndrome virus, named strain E05 which was isolated from breeding muscovy duck with irregular egg dropping was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The result showed that the nucleotide length of hexon protein gene encoding 910 amino acids was 2733 bp.

运用PCR技术从表现产蛋异常的种番鸭分离鉴定的减蛋综合征病毒 E05株中分段扩增六邻体蛋白基因，分别与克隆载体pMD18-T连接后，转化感受态细胞，对经PCR鉴定和酶切鉴定均为阳性的克隆进行测序，结果表明，E05株六邻体蛋白基因长度为2733 bp，共编码910个氨基酸。

We demonstrate that both the IL-5 mRNA expression and IL-5 protein production were stimulated by TSA and NaBu treatments. Chromatin immunoprecipitation assays showed that treatments of TSA and NaBu caused hyperacetylation of histones H3 and H4 at the IL-5 gene promoter in Jurkat cells, which consequently promoted the exogenous luciferase activity driven by this promoter.

TSA和丁酸钠能促进IL-5基因的mRNA和蛋白的表达水平，并增强该基因启动子所介导的外源荧光素酶报告基因的活性，这一增强作用与两种抑制剂介导的IL-5基因启动子上组蛋白H3和H4的高乙酰化有关。

mdm2 gene expression atmrna and protein levels,p53 protein and eb virus dna were detected by nonradioactive in situ hybridization ,immunohistochemistry and polymerase chain reaction separately in 46 cases of npc tissuesand 12 cases of chronic inflammation of nasopharyngeal epithelium.

运用非同位素原位杂交、免疫组化和多聚酶链反应技术对46例npc、12例慢性鼻咽炎症粘膜分别进行mdm2 mrna、mdm2蛋白、p53蛋白及ebv-dna的检测。

This protein reduced antibody titer in mice immunized with BSA. Following the deletion of sCD152, however, there was no obvious inhibitory effect on the primary immune response triggered by the nonrelevant antigen OVA, indicating that sCD152 had an antigen-specific immunosuppressive effect. We designed a new vector, pPIC-HE-sCD152, to get entirely native sCD152 without His-tag.

为了获得既带有纯化配体表位又带有可剪切序列的可溶性sCD152蛋白，我们设计构建了含有His-tag和肠激酶识别切割序列DDDDK的sCD152表达载体pPIC-HE-sCD152，结果这一表达载体在毕赤氏酵母中同样高效分泌表达出目的蛋白，大量纯化后用肠激酶酶切去除His-tag，用以进一步功能实验，初步结果表明具有体外细胞免疫抑制功能。

We study the expression levels of GLUT1 in 16 samples of esophageal cancer and their normal mucosa by reverse transcriptasepolymerase chain reaction and immunohistochemical staining, analyse the correlation between the expression level of GLUT1 mRNA and protein in the tumor cells. Then we study the expression levels of GLUT1 in 55 patients with esophageal cancer by immnohistochemical staining, analyse the relationship between GLUT1 expression and clinical features of the patients.

我们采用逆转录聚合酶链反应和免疫组化染色的方法，研究GLUT1 mRNA及蛋白在16例食管癌病人的肿瘤组织和相应正常食管粘膜的表达水平，同时分析食管癌组织中GLUT1在mRNA水平和蛋白水平表达的相关性；采用免疫组化染色的方法研究55例食管癌病人肿瘤标本的GLUT1表达水平，并分析其与肿瘤的临床特性及预后的关系。

In order to get CPP with lower N/P ratio, casein was first hydrolyzed with pancreatin until the DH reached 12%, and then Neutrase, a microbial protease, was added to increase the DH of casein hydrolyzate by an additional 3%.

在胰酶作用于酪蛋白的DH达12％基础上，选用微生物中性蛋白酶继续水解，使中性蛋白酶作用于酪蛋白水解液的DH达3％，以制备N/P更小的CPP。

Methods To deter mine the composition and activation of cerebroproptein hydrolysate by aminoacid analyzer,HPLC,SDS-PAG and succinate denydrogenase,to determine the therapeutic e ffectiveness of cerebrprotein hydrolysate by ordinary pressure oxytolerant exper iment and paramnesia experiment.

用氨基酸分析仪、高效液相色谱仪、SDS凝胶电泳和琥珀酸脱氢酶法测定脑蛋白水解物的组成与活性。用常压耐缺氧实验和记忆获得障碍法测定脑蛋白水解物的疗效。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

After 48 hours pre-induction, the percentage of nestin was 24%, which was 4.6% in the control group. After 48 hours induction, BMSCs exhibited the typical form of perikaryon with pyknotic cell body and prominence projected like that of neuron.

诱导48 h后，巢蛋白染色呈阳性，并可见大量神经元特异性烯醇化酶和胶质纤维酸性蛋白阳性细胞出现；对照组均呈阴性表达。

After 48 hours induction, BMSCs exhibited the typical form of perikaryon with pyknotic cell body and prominence projected like that of neuron. The cells were positively stained with NSE and GFAP in the control group.

诱导48 h后，巢蛋白染色呈阳性，并可见大量神经元特异性烯醇化酶和胶质纤维酸性蛋白阳性细胞出现；对照组均呈阴性表达。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。