酶蛋白

Purpose: to improve the solubility, emulsification and foaming ability of rice protein by enzymolysis.

为研究大米蛋白的酶法水解条件，提高大米蛋白的溶解、乳化和发泡性能。

After the fusion protein was cleaved by enterokinase, Trx and des(1-3)MGF was isolated by reverse-phase HPLC.

再对融合蛋白EK酶切， rpHPLC分离获得纯度达98%的des(1-3)MGF， SDS-PAGE电泳及质谱分析蛋白分子量与理论值相符。

III For constructing the expression vector of a fusion protein and obtain a target protein with full identity on aa sequence of a natural 13- 1,3-1 ,4-glucanase, with the recombined plasmid DNA harbouring the target gene as template, the primers designed with restriction sites for both terminals and enterokinase recognition site, followed by PCR amplification, was induced to the target gene.

为构建融合蛋白表达载体和获得与天然蛋白质序列完全一致的目的蛋白，以含有目的基因的重组质粒DNA为模板，设计引物时加入两端酶切位点及肠激酶裂解位点，通过PCR扩增引入目的基因中，测序结果表明接头和读框正确。

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

Consuming 6% gelsemium diets, the broilers grew slower and showed toxication,RBC,HB,TP,ALB,immune organ indexes percentage of ANAE+ lymphocytes. Percentage of rosette and NDVs antibody titer were reduced. The activity of LDHL,AKP,GPT were increased, but the activity of CHE decreased.In all,the results showed that the effect of GEB on blood and immune function of mice and broilers was different in doses.

而在基础日粮中添加6％的钩吻干粉，肉鸡生长缓慢，出现明显的中毒症状，并且能够降低红细胞数、血红蛋白含量、总蛋白含量、白蛋白含量、免疫器官指数、外周血ANAE~+淋巴细胞百分率、玫瑰花环形成百分率以及新城疫病毒抗体效价，机体血清酶LDHL、AKP、GPT活性升高，CHE活性降低。

The new test took only about 90 minutes and could detect gliadin in the parts per billion range.

过去溶酶检测法检测麸质用时8个小时，而现在这项新方法只需90分钟即可，它能在一千万个蛋白中迅速检测到麸朊蛋白。

Among the genes were glyceraldehyde 3-phosphate dehydrogenase, serine protease inhibitors, mitochondrion coding region, part of myosin heavy chain gene and two new genes, respectively.

结果 筛选出 6种蛋白分子基因：3-磷酸甘油醛脱氢酶，丝氨酸蛋白酶抑制剂，线粒体编码蛋白，肌球蛋白部分重链基因以及两个未知新基因。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

The diversity of influenza A arises from variations in the two proteins on its surface, called hemagglutinin and neuraminidase.

甲型流感病毒的多样性来源于其表面两种蛋白的变化，这两种蛋白就是血凝素和神经氨酸酶。

OBJECTIVE: To investigate the effects of intrauterine growth retardation caused by malnutrition during pregnancy on the acetylation of histone H3 and expression of histonedeacetylase1(HDAC1) in the hepar of the adult offspring and to explore the relationship between them.

目的：研究孕期营养不良造成的宫内生长受限(intrauterine growth retardation，IUGR)大鼠肝脏中组蛋白H3的乙酰化水平，以及组蛋白去乙酰化酶1 (histonedeacetyl-ases，HDAC1)的表达变化，探讨两者之间的相互联系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。