酶蛋白

Experiments on decalcification and activation of osteoinductive ability, comparison between the osteogenesis of demineralized bone matrix in orthotopic and in heterotopic sites, and correlation between immune response to allograft of bone and osteoinductive activity of the graft, were reported in this paper. 1. Syngeneic deminerolized bone matrix and the mixture of DBM with bone ash or acid-soluble protein extracted from demineralization was implanted into muscle pouch of mice, subsequently, the inhibition of DBM osteoinduction by bone ash or acid-soluble protein was demonstrated with histological and by means of alkaline phosphatase assay.

本文对脱钙处理使骨诱导活性表达的作用、脱钙骨基质植入不同部位的成骨表现，以及同种异体骨移植引发的免疫反应与骨诱导活性的内在关系三方面进行了研究：（1）在小鼠肌肉植入同系脱钙骨基质及其与脱钙所去除的骨矿盐和酸溶性蛋白混合物，碱性磷酸酶和组织学检查表明：骨矿盐和酸溶性蛋白具有抑制脱钙骨基质骨诱导活性的作用。

The effects of transglutaminase treatment on the properties of edible films cast from soybean protein isolates (SPI1,SPI2),sodium caseinate (NaCN1,NaCN2) and gelatin (G1,G2) were investigated.

研究了谷氨酰胺转移酶对大豆分离蛋白（SPI1和SPI2）、酪蛋白酸钠（NaCN1 和NaCN2）及明胶（G1和G2）3类蛋白质成膜特性的影响。

The possibility of crosslink between sodium caseinate and whey isolateand the impacts on functionality after crosslinking by transglutaminase and the application of transglutaminase on yoghurt were studied.

首先，利用SDS-PAGE电泳结合凝胶成像分析技术，比较了在非变性、加入还原剂(20mmoL/LDTT)变性和在80℃预热15min后并再加入一定量还原剂(20mmoL/LDTT)变性三种条件下转谷氨酰胺酶对2%酪蛋白和乳清蛋白之间的交联情况。

The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia.

实验前后用睫状肌麻痹下验光、A超动态观察豚鼠眼屈光度和眼轴长度，并取后极部巩膜组织，用电镜、终末脱氧核糖核苷酸转移酶介导原位缺口末端标记法和流式细胞术检测巩膜的凋亡细胞，应用免疫组织化学染色和蛋白印迹（Western-blot）法检测bFGF和bcl-2蛋白。

Model group and the normal group were taken distilled water,rats in the Tangshukang group were taken Tanggukang powder, western medicine control group were taken Calcium Carbonateand alpha D3. once a day for 12 weeks. Ca,P, BALP,BGP in blood and bone miner density;bone mineral contents, osseous vitodynamics,the content of Ca and P in the cremains were tested respectively.The femur was taken and detected the expression of TGF-β_1imRNA , the duodenum was taken and detected expression of VDR mRNA and CaBp-D9K mRNA with situ hybridization.

分别给予糖疏康、碳酸钙阿法迪三和生理盐水灌胃给药。12周后取材，测体重、血糖、血清钙、磷、血清骨源性碱性磷酸酶、骨T-羧基谷氨酸蛋白、骨密度、骨矿含量、骨的生物力学、骨干重、灰重、骨钙、磷等指标、组织病理切片骨形态计量学指标测定、并进行维生素D受体mRNA、转移生长因子-β_1(TGF-β_1)mRNA、钙结合蛋白(CaBp-D9K)mRNA原位杂交。

Here we show that phenylarsine oxide and diamide (both were inhibitors for protein tyrosine phosphatases), but not genistein (an inhibitor for protein tyrosine kinases), adenosine, wortmannin and LY294002 (all were inhibitors for phosphatidylinositol 3- and 4-kinases), could inhibit P-selectin exocytosis on activated platelets and could abolish the P-selectin mediated aggregation of activated platelets to neutrophils.

我们发现蛋白酪氨酸磷酸酶的抑制剂phenylarsine oxide和diamide能够抑制P-选凝素从活化的血小板中外排出来，并能够抑制P-选凝素介导的活化的血小板在白细胞表面的粘附，而蛋白酪氨酸激酶的抑制剂genistein，磷脂酰肌醇3-或4-激酶的抑制剂adenosine、wortmannin和LY294002在这些实验中没有作用。

Here we show that phenylarsine oxide and diamide (both were inhibitors for protein tyrosine phosphatases), but not genistein (an inhibitor for protein tyrosine kinases), adenosine, wortmannin and LY294002 (all were inhibitors for phosphatidylinositol 3-and 4-kinases), could inhibit P-selectin exocytosis on activated platelets and could abolish the Pselectin mediated aggregation of activated platelets to neutrophils.

我们发现蛋白酪氨酸磷酸酶的抑制剂phenylarsine oxide和diamide能够抑制P-选凝素从活化的血小板中外排出来，并能够抑制P-选凝素介导的活化的血小板在白细胞表面的粘附，而蛋白酪氨酸激酶的抑制剂genistein，磷脂酰肌醇3-或4-激酶的抑制剂adenosine、wortmannin和LY294002在这些实验中没有作用。

In the preparation of bio-imprinted enzymes, two bio-imprinting approaches are available:(1) bio-imprinting of egg albumin using heat-denaturalization; and (2) bio-imprinting of egg albumin using bovine serum albumin using dissolving-precipitation process.

在制备生物印迹酶方面，利用蛋白质在水溶液中的柔性，分别采用两种方法对蛋白质进行生物印迹：以热变性法对蛋清蛋白进行生物印迹，以及用溶解沉淀法对牛血清蛋白进行生物印迹。

Research suggests that smoking ages your skin, mostly because smoking releases an enzyme that breaks down collagen and elastin.

研究表明，吸烟会使你的皮肤老化，主要是因为吸烟释放的酶能分解胶原蛋白和弹性蛋白。

Three species of macrophyte (Elodea nuttalli, Vallinmeria spiralis, Alterranthera philoxerides), which are widely distributed in water environments, were used to perform solid-state fermentation to produce crude proteins. The effects of single-strain fermentation and mixed-strains fermentation of aquatic macrophyte on yields of their crude proteins and cellulase activities were analyzed.

采用微生物固体发酵技术对伊乐藻、苦草和喜旱莲子草3种高等水生植物进行单细胞蛋白生产的研究，分析单一菌种和混合菌种发酵过程中水生植物粗蛋白含量和纤维素酶活的变化过程。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。