酶蛋白

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

The results showed that the HRP-labelled pinellin was localized on the epitheliurri of endometrium and glandula as revealed after development with 3, 3'-diaminobenzidine–H_(2)O_(2), and the effect could be inhibited by unlabelled pinellin.

结果显示小鼠子宫内膜和腺管上皮以及胚胎外胚盘锥体部分呈现明显的酶标显色颗粒，它们的产生能被未经酶标半夏蛋白所抑制。

To date, only the structures of prokaryotic succinate:ubiquinone oxidoreductases, which share a similar enzymatic function with mitochondrial SQR, have been reported, including QFR from E.coli, QFR from Wolinella succinogenes and SQR from E.coli.

在此之前，只有原核生物的琥珀酸泛醌氧化还原酶的结构得到了解析，这包括大肠杆菌的 QFR ， Wolinella succinogenes 细菌中的 QFR 和大肠杆菌的 SQR ，这些蛋白有着与线粒体复合物 II 相类似的酶功能。

The prestent invention discloses the cDNA sequnce of a new human reduced NADH: ubiquinone oxidoreductase 14 KDa subunit (NADH-CoQ14KDa subunit). The protein coded with the sequence is the homolog of ox's NADH-CoQB14.5b subunit.

本发明提供了一种新的人还原型烟酰胺腺嘌呤二核苷酸－辅酶Q氧化还原酶14千道尔顿亚基（NADH：ubiquinone oxidoreductase 14 kDa subunit，简称为&NADH－CoQ 14 kDa亚基&的cDNA序列，该序列编码的蛋白是牛NADH－CoQB14.5b亚基的同系物。

Alternatively, we discuss the conformational retainment of enzyme in the gel and theinteraction between enzyme and gel, as well as a long range allosteric effect of the proteinresulting from the redox process of heme in the cytochrome c oxidase.

此外，对酶在凝胶中的构象保持及其相互作用，酶中血红素的氧化还原产生的长程蛋白异构效应等也给予具体地评述。

Results Group C had the symptoms such as reduced activity, acceded, indulge in lying and weight loss after 3 weeks of immune injection, 14 out of 16 SD rats in Group B had the same symptoms as Group C after 4～5 weeks of immune injection, the serum enzymes in model groups increased significantly compared with those of the control group, model group C was much higher than model group B; the duration shorted, amplitude decreased, multiphase wave increased in electromyogram of model groups; MRI examination revealed samples from model group B and C had one positive case each, which presented T1MI isodensity or hypodensity signal, T2MI and STIR serial hyperdensity signal, revealing muscle inflammation; all rats'skeletal muscle from model group C and 11 out of model group B had pathological changes, which exhibited striated muscle focal fiber degeneration, necrotized and inflammatory cell infiltration, interstitial vessel wall thickening, random cardiac muscle samples had 3 positive changes, which had similar changes to skeletal muscle, there was 1 positive change from lung sample.

结果：模型C组于免疫注射第3周左右开始出现活动减少，倦怠嗜卧，食欲体重下降等表现，模型B组有14只SD大鼠于免疫注射第4～5周出现上述症状，较C组为轻；模型组肌酶谱中，肌酸激酶、乳酸脱氢酶、谷草转氨酶与对照组比较明显升高，模型C组较模型B组升高更显著；模型组肌电图时限缩短，波幅降低，多相波增多；磁共振检查模型B组和C组选送标本中各有一例有阳性改变，表现为T1MI等或稍低信号，T2MI及STIR序列为高信号，提示肌肉炎症水肿改变；模型B组有11只，模型C组全部大鼠骨骼肌出现病理改变，表现为横纹肌局灶性分布的肌纤维变性炎细胞浸润，间质小血管壁增厚、扩张，随机选送的心肌标本中有3例有阳性改变，表现与骨骼肌相仿，选送的肺标本中有1例有阳性改变，表现为蛋白渗出，炎症细胞浸润和小血管改变。

Methods: Twenty-four male SD rats were randomly divided into normal control (NC, n=6), protein C activator (PCA, n=6), lipopolysaccharide (LPS, n=6) and LPS+PCA group (n=6). NC group were given normal saline and PCA group were injected protein C activator (0.4 mg/kg) through vein of the tail. LPS group were administered lipopolysaccharide (10 mg/kg, 4h) by intraperitoneal injection method to make the model of septic shock rats. After the animal model was made, LPS+PCA group were injected protein C activator (0.4 mg/kg) through vein of the tail. 30 minutes after the injection, each rat was tested by Medlab biosignal analyzing system for observing mean arterial pressure and left ventricle function, the activity of LDH and iNOS in myocardium and the cuttings of myocardial tissues.

取SD雄性大鼠24只随机分成对照组、PCA组、LPS组和LPS+PCA组四组，对照组尾静脉注射生理盐水，PCA组尾静脉注射蝮蛇毒蛋白C激活物，LPS组腹腔注射LPS (10 mg/kg， 4h)的方法建立败血症休克的实验动物模型，在建立败血症的实验动物模型的基础上，尾静脉注射PCA (0.4 mg/kg)为LPS+PCA组，每只大鼠均于药后30 min时运用Medlab生物信号采集处理系统记录平均动脉压和左心室功能指标，并测定心肌中乳酸脱氢酶活性、诱导性一氧化氮合酶活性及心肌病理切片。

It was found that one of the fragments was similar to the Peroxidase gene located on the rice chromosome 1 completely, another fragment was partially similar to the protein Phosphorylase gene located on the rice chromosome 12 with 99.99% homology.

同源性均在99％以上。其中一个与位于水稻第一号染色体的过氧化物酶基因的部分片段有100％相似性。另一个与位于第十二号染色体上的蛋白磷酸化酶基因有99.99％的同源性。

Objective:To investigate the effects of musk-1, a glucoprotein component isolated from the water extract of musk, on some functions of rat polymorphonuclear leukocytes activated by IL-8 in vitro.

目的：观察麝香糖蛋白成分麝香-1对白细胞介素-8(IL-8)激活的大鼠中性白细胞某些功能，包括超氧阴离子产生和溶酶体酶释放的影响。

The expression of phosphorylase gene in stigma and style may beinvolved in regulating glucose metabolism and glucoprotein synthesis during pollengermination and pollen tube growth.

磷酸化酶基因在柱头和花柱中的活跃表达可能与花粉萌发和花粉管生长过程中的葡萄糖代谢和糖蛋白合成有关；磷酸化酶可能参与雌蕊及颖果发育过程中过渡性淀粉和储藏性淀粉的合成、分解和转运。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。