酶蛋白

The research presented here is aimed at exploring the effect of DTT on the in vitro fibril formation of hen egg-white lysozymes with four disulfide bonds. In this work, we specifically addressed the effect of DTT concentration under different incubation temperatures on the hen lysozyme structure and fibril formation, the effect of addition of DTT at later time points on the amyloid fibrillization of hen lysozymes, and the effect of concentration ratio between reduced and oxidized forms of DTT on the fibril formation of lysozymes.

本研究以具有四对双硫键的母鸡蛋白溶菌酶为蛋白质模式系统，在含盐类之酸性溶液环境下诱导生成类淀粉纤维，再配合二硫代苏糖醇破坏溶菌酶中的双硫键，探讨：（1）不同温度下加入不同DTT浓度对溶菌酶类淀粉纤维形成之影响，（2）不同生长时期加入DTT对溶菌酶类淀粉纤维形成之影响，（3）不同比例之DTT和氧化态DTT浓度对溶菌酶类淀粉纤维形成之影响。

Compared to wild-type plants, the onset of flowering was advanced. The results of this study may be useful in the mulberry gene engineering and the results may be helpful to study the regulation of SBPase.Rubisco activase is a soluble chloroplast protein, coded in nucleus, and has the activation of Rubisco in photosynthetic autotrophs.

本研究为桑树基因工程提供了有效的候选基因，为深入研究SBPase的分子调控机制奠定了基础。1，5-二磷酸核酮糖羧化酶活化酶广泛存在于光合生物中，它是一种由核基因编码的叶绿体蛋白，具有调节Rubisco活性的功能。

The activities of catalase,glutathione peroxidase,superoxide dismutase and protein contents in renal homogenates of rats exposed to TNT and treated with clofibrate were observed at present study.

内容摘要本研究观察了三硝基甲苯不同剂量染毒和阳性对照安妥明处理对大鼠肾组织匀浆过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶活性及肾组织蛋白含量的影响。

W. . The levels of myocardial troponin I, creatin kinase, aspartate transamnaseand lactatede hydrogenasewere detected before and per 30 mins during the experiment, and the changes of rabbit myocardial pathology were analyzed.

分别于实验前和实验中每隔30分钟抽取血液，以肝素抗凝并分析心肌肌钙蛋白I浓度，肌酸激酶、门冬氨酸氨基转移酶和乳酸脱氢酶活性，同时观察心肌组织的病理学变化。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

The results indicated that the paralysors and excitomotors of calcium channel changed the expression level of lipoprotein lipase, peroxisome proliferators-activated receptor gamma, fatty acid synthetase, and the lipid accumulation, markedly.

结果表明：钙通道阻滞剂和激动剂显著改变了脂蛋白脂酶，过氧化物增殖激活受体γ、脂肪酸合成酶的表达水平，且影响细胞内的脂质蓄积。

AIM To study the direct effect of tyrphostin AG114 on recombinant human protein kinase CK2 holoenzyme and its kinetics.

目的为了观察Tyrphostin AG114对重组人蛋白激酶CK2全酶的直接作用及其酶动力学机理。

Neutral proteinase was used to hydrolyse Acetes chinensis protein to prepare active peptides, which could inhibit the activity of angiotensin I-converting enzyme.

采用3942中性蛋白酶酶解中国毛虾蛋白，制备具有抑制血管紧张素转移酶活性的多肽。

The invention provides a method when alkaline protease hydrolyses from big molecular collagen into minor polypeptides, free amino acids in enzymatic hydrolysate increase gradually, a positive correlation is shown between increased amount and hydrolysis degree, so the hydrolysis degree can be calculated by detecting total amino acids or a certain kind of amino acid content in enzymolysis liquid.

本方法是利用碱性蛋白酶水解大分子胶原蛋白生成小分子多肽的同时，酶解产物里游离氨基酸含量逐渐增加，其增加的量与水解度大小呈正相关，因此，通过检测酶解液中氨基酸总量或某一种氨基酸含量可计算出水解度的大小。

RESULTS: Wound healing rate, wound healing time, histopathology analysis, quantity assay of macrophage, determination of hydroxyproline, proliferation of cell, assay of DNA contents and circle of cells, level of transforming growth factor-alpha, levels of interleukin-1, interleukin-6 and tumor necrosis factor, assay of keratinocyte collagenase-1, level of fibroblast growth factor receptor-1, level of monocyte chemoattractant protein-1 and level of keratinocyte plasminogen activator inhabitor type 2 were selected as the evaluation criteria of wound healing.

结果 筛选了创面愈合率、创面愈合时间、组织病理学分析、巨噬细胞定量分析、羟脯氨酸含量测定、细胞增殖情况、细胞DNA含量和细胞周期分析、转化生长因子-α水平、白细胞介素-1、白细胞介素-6和肿瘤坏死因子水平、角质细胞胶原酶-1含量测定、成纤维细胞生长因子受体-1水平、单核细胞化学诱导蛋白-1水平和角质细胞纤溶酶原活化抑制剂-2水平等十三种创面愈合评价指标。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。