酶蛋白

The samples of decalcified bone matrix, composite material and recombinant human bone morphogenetic protein 2 were respectively co-incubated with C2C12 cells for 72 hours.

细胞裂解后用比色法分别检测裂解液中成骨细胞特异性标记物碱性磷酸酶及总蛋白的吸光度值，以两者的比值表示单位数量的C2C12细胞所含有的碱性磷酸酶含量，以此衡量待测生物材料成骨活性的高低。

All these three methods could inactivate LOX with the low extent of protein denaturing.

以上三种灭酶方法都能够保证蛋白变性程度不高的条件下实现对脂肪氧合酶的灭活。

The effect of extraction time, enzyme concentration and liquid to solid ratio on the protein hydrolyzed was studied.

结果表明：在同样时间、加酶量和料液比，微波－酶法耦合水解大豆蛋白的水解度均比常规法高。

The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.

分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme，HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme，HdvRz)，并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游，构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT，经Plasmid Midi Kit纯化后，在Lipofectamine~ 2000的介导下，直接转染DFI细胞，72h后取上清继续在CEF上传代。

With the orthogonal test, the optimal reaction conditions ofα-amylase and saccharified enzyme were obtained.

试验中采用无水乙醇脱脂，优选出的最佳蛋白水解酶为木瓜蛋白酶，通过正交试验确定了α-淀粉酶和糖化酶的最佳水解条件。

In promoters, exons and partially introns of three SODs, 59 SNPs were discovered.

酶活分析表明，重组AiCuZnSOD蛋白有较高的酶活和稳定性。

The effects of gastrodine on the expression of glial fibrillary acidic protein, the transuding amount of lactic dehydrogenase, and the activity of nitric oxide synthase in cultured astrocytes of young rats with lesions caused by simulated cerebral ischemia and reperfusion, were determined in this experiment.

观察天麻素对脑缺血再灌注损伤星形胶质细胞胶原纤维酸性蛋白表达、乳酸脱氢酶漏出量以及一氧化氮合成酶活性的影响。结果：天麻素大、小剂量组GFAP纤维样改变减轻，强阳性反应细胞数减少；LDH漏出量下降；NOS活性减弱。

These results suggest that the Aeromonas caviae CB101 can only secrete one kind of chitinases ,which are Chi1 and its truncates.

基因克隆和蛋白分离、纯化实验都暗示CB101的几丁质酶系统只包含一个几丁质酶基因。

Serum alt, ast, bile acid and albumin levels were measured with automatic biochemistry analysator during the procedure.

采用ccl4造模，全自动生化分析仪检测血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、胆汁酸及白蛋白水平；he染色和vg染色观察肝脏病理及纤维化面积的变化，分析黄芪对肝功能及肝纤维化形成的影响。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二酰基甘油酰基转移酶(Diacylglycerol acyltransferase， DGAT)基因，硬脂酰-酰基载体蛋白脱饱和酶(Acyl- desaturase， SAD)基因克隆测序，经测序，通过NCBI与已知的核苷酸序列进行同源性比对，结果表明：克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。