酶蛋白

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Changes of serum concentration of LDL-C,HDL-C,LPO and LDH isoenzymes have been studied in 35 cases of esophagus cancer,13 cases of lung cancer and 5cases of breast cancer before radiotherapeutics and chemotherapy.

本文对未进行放疗及化疗的53名癌瘤患者(食管癌35例、肺癌13例、乳腺癌5例)进行了血清脂蛋白亚组分中胆固醇含量(LDL-C、HDL-C)及脂质过氧化物和乳酸脱氢酶同工酶的研究。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8，酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2（IL-2）和Th2型细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）水平，逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达；ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

Sodium lauroyl sarcosine was used as a denaturant, a novel protein refolding method, which was proposed on the basis of one step-AMC and IEC, one step-AMC-IEC was used on the refolding of denatured/reduced lysozyme.

一步人工分子伴侣-离子交换色谱法辅助还原变性溶菌酶的复性：以月桂酰基肌氨酸钠(Sodium Lauroyl Sarcosine，Sarkosyl，SLS)作为蛋白变性剂，发展了一步人工分子伴侣-离子交换色谱法，对还原变性溶菌酶进行了复性研究。

Armigera and M. separata mid-gut juice, but, other less than 60kD proteins also can be got by M. separata mid-gut juice .4 Many Bt Cry1AcE toxins can be got by the method of precipitate and resolve, which have high purity.

在相同的时间下，粘虫中肠消化液蛋白酶活性比棉铃虫中肠消化液要高；在37℃条件下，棉铃虫中肠消化液经过30和60min将130kD的Bt CrylAc原毒素酶解为60kD的毒素，而粘虫中肠消化液经过30和60min对Bt CrylAc原毒素酶解作用，除得到60kD的毒素外还得到了40kD和分子量更小的蛋白。

Hideyuki Doi, Nakui Madaki, Hrroshi, Hiromichi Komatsu, Keisei Fujimori and Susumu Satomi Tohoku. A New Preadipocyte Cell Line,AP-18,Established from Adult Mouse Adipose Tissue. J.Exp.Med,2005,207:209-216[2] Tontonoz P, Hu E, Spiegelman BM. Stimulation of adipogenesis in fibroblasts by PPARγ 2, a lipid-activated transcription factor. Cell ,1994,79:1147－1156

PPARα调节脂肪代谢的机制总结如下：肝脏脂肪酸水平的增高激活PPARα，然后转录激活参与脂肪酸氧化的基因；配体与之结合后可激活编码乙酰辅酶A合成酶的基因，同时PPARα激活后可调节脂肪转运相关基因的表达如PPARα升高高密度脂蛋白和抑制ApoC－Ⅲ的表达。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

To enhance the utilization of raw materials and Tenebrio molitor protein extraction rate, selection of these two enzymes for the enzyme extraction.

为提高原料利用率和黄粉虫蛋白提取率，选择这两种酶为提取用酶。

Objective: To investigate the expression and their correlation of P-gp,GST-π,TopoⅡ and p53 in breast carcinoma.

目的：探讨乳腺癌中P-糖蛋白，谷胱甘肽-S-转移酶，拓扑异构酶和P53的表达及相关性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。