酶蛋白

To mix the bean d regs to-gether with water ,Enzym Ⅰand Enzym Ⅱin proportion as 1 00 :1 00～150:0.2～0. 25 : 0.15～0.2, and after the fermentation for 2～3h,it will become protein food bydrying it up.

把鲜豆渣和水、酶Ⅰ、酶Ⅱ按100：100～150：0.20～0.25：0.15～0.20的重量比混合后，在适合的温度，pH条件下搅拌发酵2～3小时，干燥粉碎制成粉末蛋白食品。

It was difficult to extract corn pigments completely, for some of them were in a protein-bind state. It was better to use active carbon for the decolorization of GEON, the conditions were 40℃, stir time 6 hours, the ratio of carbon to hydrolyzate 1: 100 , but as to milk-like powder, it was better to extract pigments after spray drying.

最后，本文对玉米黄素的可抽提性也进行了初步研究，结果表明，部分色素与蛋白质结合而难以抽提，因而有机溶剂难以将色素抽提完全，对Gln活性肽营养液而言，可选用酶解后加活性炭进行脱色，脱色条件为40℃，搅拌6小时，炭液比为1∶100；而对黄粉蛋白乳粉而言，喷雾干燥后用乙酸乙酯脱色，可使酶解干燥后黄粉的异味减轻。

Telomere, a complex structure present at the ends of eukaryotic chromosomes, consists of tandam arrays of highly conserved hexameric repeats in vetebrates. Its function is to protect DNA from degradation of deleterious recombination.

端粒是由许多简单重复序列及相关蛋白组成的染色体末端复合结构，端粒酶是具有逆转录酶特性的核糖核蛋白复合物，能以自身的RNA为模板合成端粒重复序列，加至新合成的DNA链末端，维持端粒平衡从而保证染色体末端结构完整性及解决末端复制问题。

Until now, the genes related to apoptosis of Bombyx mori which have logged in NCBI includes the BmP109, BmIap, BmCdc2, BmIce, BmIce2, BmIcad and 6G1 gene. Thereinto, the apoptotic function of the homologic genes of BmIce in Homo sapiens, drosophila and C.elegans have been identified. The aim of this study was to identify the function of the interleukin-1 beta converting enzyme of Bombyx mori, BmIce.

目前已在NCBI上登录克隆序列的家蚕细胞凋亡相关基因有：含Bcl-2家族同源域BH结构域的BmP109，凋亡抑制因子BmIap，生存因子途径中的BmCdc2，家蚕白介素转换酶BmIce和BmIce2，caspase活化的DNA酶抑制剂BmIead基因和30k蛋白中的6G1基因。

Mutant libraries created by saturation mutagenesis of each single amino acid site D168, A225, K434 and E435 were screened to further improve the capability of cytochrome P450 BM-3(A74G/F87V/L188Q) mutant from Bacillus Megaterium which can hydroxylate indole into indigo. Results showed that D168, K434 and E435 are located at the functional domain of P450 BM-3 protein while A225 is sited at the unfunctional domain.

为了进一步获得具有更高活力的细胞色素P450 BM-3(F87V/A74G/L188Q)突变酶，利用饱和突变技术对该突变酶的4个氨基酸位点D168、A225、K434、E435分别进行单一的随机突变，通过对其羟基化吲哚生成靛蓝的催化性能表征，发现P450 BM-3氨基酸残基的168、434和435位均位于蛋白功能区域，而225位则位于非功能区域。

The results indicates that enzyme combination diluent is suited for the production of HCV antibody enzyme linked immunosorbent diagnostic reagents,if the enzyme combination diluent regards PBST as buffer liquid and BSA and Casein irrelated albumen as stability .

结论以PBST为缓冲液，以BSA和C as ein无关蛋白作稳定剂组成酶结合物稀释液适用于HCV抗体酶联免疫诊断试剂的生产。

These metabolomic findings were substantiated by proteomic experiments demonstrating differential expression of 3-oxoacid transferase, the key enzyme for ketolytic energy production.

这些代谢发现被蛋白组学实验证实，后者证明3－酮酸转移酶－解酮和能量产生的关键酶，差异表达。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

The sequence had 80% identity with L1PR10.1C, a PR010 protein from Lupinus luteus. AmPR 10had ribonuclease activity of 74.11 U/mg protein, as some other PR 10 proteins.

同时，AmPR 10具有核糖核酸酶活性，纯AmPR 10的核糖核酸酶比活性为74.11 U/mg，这点与一些PR 10蛋白类似。

Prophenoloxidase is a key enzyme for mediating melanotic encapsulation of oocysts,which may be transformed into active phenoloxidase by limiting proteolysis.

而前酚氧化酶(Prophenoloxidase，PPO)是介导卵囊黑化包被反应的一种关键酶，活化后可催化酪氨酸羟基化为二羟基苯基丙氨酸，二羟基苯基丙氨酸和多巴胺可进一步氧化为具有活性的苯醌活性，苯醌即可介导蛋白交联并聚合成黑色素层固定、隔离或杀死入侵的病原体。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。